FYP Word Scramble
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| Term | Definition |
| DGAT1 | The diacylglycerol O-acyltransferase (DGAT1) is a microsomal enzyme that catalyzes the final step of triglyceride synthesis. |
| K232A | An association between lysine at amino acid position 232 with elevated milk fat content, while an alanine at this position is associated with lowered milk fat content |
| Polymorphism | Any difference in the nucleotide sequence- Variations |
| Conventional PCR (Polymerase Chain Reaction) | A technique that allows exponential amplification of DNA sequences. Genes are targeted with complimentary primers to the specific sequence of DNA and amplified with an enzyme known as polymerase |
| RFLP (Restricted Fragment Length Polymorphism) | A technique that finds variations in DNA by using an enzyme to break down the DNA different sizes depending on its specific code, the process is then carried to Gel Electrophoresis |
| Gel Electrophoresis | Separates DNA fragment made by RFLP by size and charge. Longer pieces of DNA stay close to wells, shorter DNA ,moves closer to positive charge creating unique fingerprint. |
| DNA Sequencing | DNA is cut and inserted into plasmid DNA, this is then in bacterial cells. DNA is isolated and added to a sequencing plate. DNA basses are added, polymerase is added, DNA primers, and fluorescent terminator bases are added.PCR then Electrophoesis occurs |
| Real-time PCR (Polymerase Chain Reaction) | |
| SYBR Green Assays | S |
| SNP(Single Nucleotide Polymorphism) Variants | G |
| Exon 8 of chromosome 14 | I |
| Complimented Primers | G |
| Polymerase | N |
| Thermocycler Denaturation | X |
| Annealing | Y |
| Extension | J |
| 411bp fragment | G |
| Restriction sites for a restriction Enzyme | S |
| CFR1 | S |
| Sanger Sequencing | C |
| 10433-10444 position of the K232A Polymorphism | V |
| Fluorescent reporter molecules | Q |
| Threshold Cycle | C |
| TaqMan allelic discrimination kit | H |
| ZR Genomic DNA II Tissue Mini prep kit | H |
| Clean and Concentrator -25TM Kit | Q |
| Nucleotide | The building blocks of nucleic acids; they are composed of three subunit molecules: a nitrogenous base, a five-carbon sugar (ribose or deoxyribose), and at least one phosphate group |
Created by:
MarkA0845
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