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DGAT1 The diacylglycerol O-acyltransferase (DGAT1) is a microsomal enzyme that catalyzes the final step of triglyceride synthesis.
K232A An association between lysine at amino acid position 232 with elevated milk fat content, while an alanine at this position is associated with lowered milk fat content
Polymorphism Any difference in the nucleotide sequence- Variations
Conventional PCR (Polymerase Chain Reaction) A technique that allows exponential amplification of DNA sequences. Genes are targeted with complimentary primers to the specific sequence of DNA and amplified with an enzyme known as polymerase
RFLP (Restricted Fragment Length Polymorphism) A technique that finds variations in DNA by using an enzyme to break down the DNA different sizes depending on its specific code, the process is then carried to Gel Electrophoresis
Gel Electrophoresis Separates DNA fragment made by RFLP by size and charge. Longer pieces of DNA stay close to wells, shorter DNA ,moves closer to positive charge creating unique fingerprint.
DNA Sequencing DNA is cut and inserted into plasmid DNA, this is then in bacterial cells. DNA is isolated and added to a sequencing plate. DNA basses are added, polymerase is added, DNA primers, and fluorescent terminator bases are added.PCR then Electrophoesis occurs
Real-time PCR (Polymerase Chain Reaction)
SYBR Green Assays S
SNP(Single Nucleotide Polymorphism) Variants G
Exon 8 of chromosome 14 I
Complimented Primers G
Polymerase N
Thermocycler Denaturation X
Annealing Y
Extension J
411bp fragment G
Restriction sites for a restriction Enzyme S
Sanger Sequencing C
10433-10444 position of the K232A Polymorphism V
Fluorescent reporter molecules Q
Threshold Cycle C
TaqMan allelic discrimination kit H
ZR Genomic DNA II Tissue Mini prep kit H
Clean and Concentrator -25TM Kit Q
Nucleotide The building blocks of nucleic acids; they are composed of three subunit molecules: a nitrogenous base, a five-carbon sugar (ribose or deoxyribose), and at least one phosphate group
Created by: MarkA0845