LAB PROCEDURES 1 NOTES
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| RBC | ERYTHROPOIESIS
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| ERYTHROPOIESIS | ERYTHROCYTE
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| LEUKOPOIESIS | LEUKOCYTE
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| LEUKOCYTE | WHITE BLOOD CELL
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| THROMBOPOIESIS | PLATELETS
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| PLATELETS | THROMBOCYTES
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| PLASMA | SPUN DOWN GREEN TOP TUBE
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| SERUM | BLOOD CLOTS AND THEN SPUN DOWN
TIGER TOP OR RED TOP TUBE
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| NEED TO CHECK TEST BEFORE PICKING | ANTICOAGULANTS
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| IF YOU DON'T USE THE BLOOD WITHIN 1 HOUR YOU SHOULD PUT THE BLOOD | IN THE REFRIDGERATOR
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| IF BLOOD IS REFRIGERATED YOU MUST | BRING THE BLOOD BACK TO ROOM TEMP BEFORE USE
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| YOU CAN'T FREEZE BLOOD BECAUSE | LYSING CELLS (BREAK APART)
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| HEPARIN | ANTICOAGULANT
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| EDTA | ETHYLENEDIAMINE TETRAOCETIC ACID
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| BLUE TOP TUBES | OXALATES & CITRATES
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| SODIUM FLOURIDE | GLUCOSE PRESERVATIVE (DIABETIC)
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| SAMPLE VOLUME | WELL HYDRATED PT WILL MAKE HALF THE AMOUNT OF PLASMA/SERUM DRAWN UP
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| CBC | COMPLETE BLOOD COUNT
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| WHICH IS BETTER MANUAL OR AUTOMATED | BOTH IS BEST
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| CBC= | TOTAL RBC, PCV, PLASMA PROTEIN CONCENTRATE (TP), TOTAL WBC, BLOOD FILM EXAM, DIFFERENTIAL WBC, RDC & WBC MORPHOLOGY, AND PLT TEST
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| READ PCV FIRST THE BREAK TUBE ABOVE | THE BUFFY COAT TO GET PLASMA
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| CLEAR TO LIGHT YELLOW | PLASMA
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| PLASMA WILL BE PINK AND CLOUDY IF THEY | JUST ATE
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| PENIA | DECREASE NUMBER OF CELLS IN THE BLOOD
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| PHILIA/CYTOSIS | INCREASES NUMBER OF CELLS IN THE BLOOD
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| LEFT SHIFT | INCREASED NUMBER OF IMMATURE NEUTROPHILS IN THE BLOOD
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| LEFT SHIFT | INCREASED NUMBER OF IMMATURE NEUTROPHILS IN THE BLOOD
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| LEUKEMIA | NEOPLASTIC CELLS IN BLOOD OR MARROW
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| LEUKEMIA | NEOPLASTIC CELLS IN BLOOD OR MARROW
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| LEUKEMOID RESPONSE | CONDITION THAT CAN BE MISTAKEN FOR LEUKOMIA
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| LEUKEMOID RESPONSE | CONDITION THAT CAN BE MISTAKEN FOR LEUKOMIA
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| NEUTROPHIL | IRREGULAR NUCLEUS-> IRREGULAR ENLONGATED WITH 3-5 LOBES
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| NEUTROPHIL | IRREGULAR NUCLEUS-> IRREGULAR ENLONGATED WITH 3-5 LOBES
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| BAND NEUTROPHIL | IMMATURE NEUTROPHIL LOOKS LIKE A HORSESHOE,
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| BAND NEUTROPHIL | IMMATURE NEUTROPHIL LOOKS LIKE A HORSESHOE,
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| BAND NEUTROPHIL | NOT COMMON IN NORMAL ANIMALS
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| BAND NEUTROPHIL | NOT COMMON IN NORMAL ANIMALS
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| LYMPHOCYTE | LARGE NUCLEUS, LIGHT BLUE CYTOPLAMS
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| LYMPHOCYTE | LARGE NUCLEUS, LIGHT BLUE CYTOPLAMS
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| LYMPHOCYTE | AGRANULAR
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| LYMPHOCYTE | AGRANULAR
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| MONOCYTE | LARGEST WBC
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| MONOCYTE | LARGEST WBC
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| MONOCYTE | VARIABLY SHAPED NUCLEI, WITH VACUOLES, AND AGRANULAR
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| MONOCYTE | VARIABLY SHAPED NUCLEI, WITH VACUOLES, AND AGRANULAR
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| EOSINOPHIL | COMMONLY STAIN RED TO PINK, MUCH LIGHTER THAN THE NUCLEUS
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| EOSINOPHIL | COMMONLY STAIN RED TO PINK, MUCH LIGHTER THAN THE NUCLEUS
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| EOSINOPHIL | TRIGGERED BY HISTAMINE RELEASE FROM MAST CELLS--ALLERGIES
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| EOSINOPHIL | TRIGGERED BY HISTAMINE RELEASE FROM MAST CELLS--ALLERGIES
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| BASOPHIL | NUCLEUS IS SIMILAR TO MONOCYTES.
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| BASOPHIL | GRANULAR
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| BASOPHIL | GRANULES STAIN DARKER THAN THE NUCLEUS DARK BUT TO BLUE BLACK.
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| BASOPHIL | TRIGGERED BY RELEASE OF EOSINOPHILS AND ALLERGIES
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| MORPHOLOGIC ABNORMALITIES SEEN IN | WBC'S
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| BIRMAN CAT NEUTROPHIL GRANULATION ANOMALLY | WILL HAVE FINE EOSINOPHILIC TO MAGENTA GRANULES
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| BIRMAN CAT NEUTROPHIL | INHERITED RECESSIVE TRAIT
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| BIRMAN CAT NEUTROPHIL | NEUTROPHIL FUNCTION IS NORMAL & AFFECTED CATS ARE HEALTHY
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| LARGE NUMBER OF BASKET CELLS = | LEUKEMIA
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| ROULEAUX | GROUP OF RBC'S IN STACKS, LOOKS LIKE STACKS OF COINS
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| ROULEAUX | COMMONLY SEEN IN HORSES
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| ROULEAUX | CAN BE SEEN IN BLOOD HELP TOO LONG OR REFRIGERATED BLOOD
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| AGGLUTINATION | OCCURS IN IMMUNE MEDIATED DISORDERS
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| AGGLUTINATION | CLUMPING OR BRIDGING OF RBC'S LOOKS LIKE A CLUSTER OF GRAPES
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| SALINE TEST | ROULEAUX WILL SEPERATE
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| SALINE TEST | AGGLUTINATION WILL NOT SEPERATE
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| ANISOCYTOSIS | VARIATION IN SIZE OF RBC'S MACROCYTES & MICROCYTES
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| POLYCHROMASIA | BLUE TINT
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| HYPOCHROMASIA | DECREASED STAINING DEFICIENT IN IRON- LOOKS LIKE CHEERIOS
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| HYPERCHROMASIA AKA SPHEROCYTES | LOOK LIKE A BALL
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| POIKILOCYTES ->ABNORMAL | NOT A Dx JUST A GENERAL STATEMENT OF RBC'S
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| SCHISTOCYES-> FRAGMENTS | DUE TO SOME TYPE OF INTRAVASCULAR TRAUMA, DIC, HEMANGIOSARCOMA
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| ACANTHROCYTES-> SPUR CELLS | ALTERED LIPID METABOLISM AND LIVER Dx
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| ECHINOCYTES | BURR CELLS
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| PREPANOCYTES-> SICKLE CELLS | BLOOD OF NORMAL DEER AND ANGORAGOALS
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| KERATOCYTES | HELMET OR BLISTER CELLS
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| SPHEROCYTES | LOOKS LIKE A BALL
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| CODOCYTES | TARGET CELLS, FOLDED CELLS
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| NUCLEATED ERYTHROVYTES | COMMONLY SEEN REGENERATIVE ANEMIA
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| BASOPHILIC STIPPLING | SMALL, DARK BLUE BODIES WITHIN RBC LEAD POISONING IN DOGS
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| HOWELL JOLLY BODIES | REMNANT SEEN IN YOUNG RBC'S
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| HEINZ BODIES | REPRESENTING DENATURED HEMOGLOBIN'S CAUSED BY CERTAIN OXIDANT DRUGS OR CHEMICALS
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| RETICULOCYTES | IMMATURE RBC'S THAT CONTAIN ORGANELLES
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| HAEMOBARTONELLA FELIS | PARASITE
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| HAEMOBARTONELLA FELIS | COMMON IN CATS
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| HAEMOBARTONELLA FELIS | AKA FELINE INFECTIOUS ANEMIA, USUALLY LOOK LIKE SHORT RODS IN PERIPHERY OF RBC'S
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| EHRLICHIA | APPEARS AS SMALL CLUSTERS IN CYTOPLAMS
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| EPERY | SWINE, CATTLE, LAMAS, SIMILAR LOOKING TO HAEMOBARTONELLA FELIS
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| ANAPLASM | SMALL, DARK STAINING COCCI AT MARGIN OF RBC
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| CYTAUXZOON FELIS | RARE CAUSE OF HEMOLYTIC ANEMIA
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| BABESIOSIS | TEXAS TICK FEVER, REDWATER FEVER, CATTLE TICK FEVER, TEAR DROP SHAPED
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| MATERIALS NEEDED FOR A BLOOD SMEAR | CLEAN SLIDES, BLOOD FROM A PURPLE TOP TUBE ->EDTA, CAPILLARY TUBE, SPREADER SLIDE, TRIPLE STAIN
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| BLOOD SMEAR | FEATHERED EDGE 2/3 UP THE SLIDE
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