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LAB PROCEDURES NOTES

LAB PROCEDURES 1 NOTES

QuestionAnswer
RBC ERYTHROPOIESIS
ERYTHROPOIESIS ERYTHROCYTE
LEUKOPOIESIS LEUKOCYTE
LEUKOCYTE WHITE BLOOD CELL
THROMBOPOIESIS PLATELETS
PLATELETS THROMBOCYTES
PLASMA SPUN DOWN GREEN TOP TUBE
SERUM BLOOD CLOTS AND THEN SPUN DOWN TIGER TOP OR RED TOP TUBE
NEED TO CHECK TEST BEFORE PICKING ANTICOAGULANTS
IF YOU DON'T USE THE BLOOD WITHIN 1 HOUR YOU SHOULD PUT THE BLOOD IN THE REFRIDGERATOR
IF BLOOD IS REFRIGERATED YOU MUST BRING THE BLOOD BACK TO ROOM TEMP BEFORE USE
YOU CAN'T FREEZE BLOOD BECAUSE LYSING CELLS (BREAK APART)
HEPARIN ANTICOAGULANT
EDTA ETHYLENEDIAMINE TETRAOCETIC ACID
BLUE TOP TUBES OXALATES & CITRATES
SODIUM FLOURIDE GLUCOSE PRESERVATIVE (DIABETIC)
SAMPLE VOLUME WELL HYDRATED PT WILL MAKE HALF THE AMOUNT OF PLASMA/SERUM DRAWN UP
CBC COMPLETE BLOOD COUNT
WHICH IS BETTER MANUAL OR AUTOMATED BOTH IS BEST
CBC= TOTAL RBC, PCV, PLASMA PROTEIN CONCENTRATE (TP), TOTAL WBC, BLOOD FILM EXAM, DIFFERENTIAL WBC, RDC & WBC MORPHOLOGY, AND PLT TEST
READ PCV FIRST THE BREAK TUBE ABOVE THE BUFFY COAT TO GET PLASMA
CLEAR TO LIGHT YELLOW PLASMA
PLASMA WILL BE PINK AND CLOUDY IF THEY JUST ATE
PENIA DECREASE NUMBER OF CELLS IN THE BLOOD
PHILIA/CYTOSIS INCREASES NUMBER OF CELLS IN THE BLOOD
LEFT SHIFT INCREASED NUMBER OF IMMATURE NEUTROPHILS IN THE BLOOD
LEFT SHIFT INCREASED NUMBER OF IMMATURE NEUTROPHILS IN THE BLOOD
LEUKEMIA NEOPLASTIC CELLS IN BLOOD OR MARROW
LEUKEMIA NEOPLASTIC CELLS IN BLOOD OR MARROW
LEUKEMOID RESPONSE CONDITION THAT CAN BE MISTAKEN FOR LEUKOMIA
LEUKEMOID RESPONSE CONDITION THAT CAN BE MISTAKEN FOR LEUKOMIA
NEUTROPHIL IRREGULAR NUCLEUS-> IRREGULAR ENLONGATED WITH 3-5 LOBES
NEUTROPHIL IRREGULAR NUCLEUS-> IRREGULAR ENLONGATED WITH 3-5 LOBES
BAND NEUTROPHIL IMMATURE NEUTROPHIL LOOKS LIKE A HORSESHOE,
BAND NEUTROPHIL IMMATURE NEUTROPHIL LOOKS LIKE A HORSESHOE,
BAND NEUTROPHIL NOT COMMON IN NORMAL ANIMALS
BAND NEUTROPHIL NOT COMMON IN NORMAL ANIMALS
LYMPHOCYTE LARGE NUCLEUS, LIGHT BLUE CYTOPLAMS
LYMPHOCYTE LARGE NUCLEUS, LIGHT BLUE CYTOPLAMS
LYMPHOCYTE AGRANULAR
LYMPHOCYTE AGRANULAR
MONOCYTE LARGEST WBC
MONOCYTE LARGEST WBC
MONOCYTE VARIABLY SHAPED NUCLEI, WITH VACUOLES, AND AGRANULAR
MONOCYTE VARIABLY SHAPED NUCLEI, WITH VACUOLES, AND AGRANULAR
EOSINOPHIL COMMONLY STAIN RED TO PINK, MUCH LIGHTER THAN THE NUCLEUS
EOSINOPHIL COMMONLY STAIN RED TO PINK, MUCH LIGHTER THAN THE NUCLEUS
EOSINOPHIL TRIGGERED BY HISTAMINE RELEASE FROM MAST CELLS--ALLERGIES
EOSINOPHIL TRIGGERED BY HISTAMINE RELEASE FROM MAST CELLS--ALLERGIES
BASOPHIL NUCLEUS IS SIMILAR TO MONOCYTES.
BASOPHIL GRANULAR
BASOPHIL GRANULES STAIN DARKER THAN THE NUCLEUS DARK BUT TO BLUE BLACK.
BASOPHIL TRIGGERED BY RELEASE OF EOSINOPHILS AND ALLERGIES
MORPHOLOGIC ABNORMALITIES SEEN IN WBC'S
BIRMAN CAT NEUTROPHIL GRANULATION ANOMALLY WILL HAVE FINE EOSINOPHILIC TO MAGENTA GRANULES
BIRMAN CAT NEUTROPHIL INHERITED RECESSIVE TRAIT
BIRMAN CAT NEUTROPHIL NEUTROPHIL FUNCTION IS NORMAL & AFFECTED CATS ARE HEALTHY
LARGE NUMBER OF BASKET CELLS = LEUKEMIA
ROULEAUX GROUP OF RBC'S IN STACKS, LOOKS LIKE STACKS OF COINS
ROULEAUX COMMONLY SEEN IN HORSES
ROULEAUX CAN BE SEEN IN BLOOD HELP TOO LONG OR REFRIGERATED BLOOD
AGGLUTINATION OCCURS IN IMMUNE MEDIATED DISORDERS
AGGLUTINATION CLUMPING OR BRIDGING OF RBC'S LOOKS LIKE A CLUSTER OF GRAPES
SALINE TEST ROULEAUX WILL SEPERATE
SALINE TEST AGGLUTINATION WILL NOT SEPERATE
ANISOCYTOSIS VARIATION IN SIZE OF RBC'S MACROCYTES & MICROCYTES
POLYCHROMASIA BLUE TINT
HYPOCHROMASIA DECREASED STAINING DEFICIENT IN IRON- LOOKS LIKE CHEERIOS
HYPERCHROMASIA AKA SPHEROCYTES LOOK LIKE A BALL
POIKILOCYTES ->ABNORMAL NOT A Dx JUST A GENERAL STATEMENT OF RBC'S
SCHISTOCYES-> FRAGMENTS DUE TO SOME TYPE OF INTRAVASCULAR TRAUMA, DIC, HEMANGIOSARCOMA
ACANTHROCYTES-> SPUR CELLS ALTERED LIPID METABOLISM AND LIVER Dx
ECHINOCYTES BURR CELLS
PREPANOCYTES-> SICKLE CELLS BLOOD OF NORMAL DEER AND ANGORAGOALS
KERATOCYTES HELMET OR BLISTER CELLS
SPHEROCYTES LOOKS LIKE A BALL
CODOCYTES TARGET CELLS, FOLDED CELLS
NUCLEATED ERYTHROVYTES COMMONLY SEEN REGENERATIVE ANEMIA
BASOPHILIC STIPPLING SMALL, DARK BLUE BODIES WITHIN RBC LEAD POISONING IN DOGS
HOWELL JOLLY BODIES REMNANT SEEN IN YOUNG RBC'S
HEINZ BODIES REPRESENTING DENATURED HEMOGLOBIN'S CAUSED BY CERTAIN OXIDANT DRUGS OR CHEMICALS
RETICULOCYTES IMMATURE RBC'S THAT CONTAIN ORGANELLES
HAEMOBARTONELLA FELIS PARASITE
HAEMOBARTONELLA FELIS COMMON IN CATS
HAEMOBARTONELLA FELIS AKA FELINE INFECTIOUS ANEMIA, USUALLY LOOK LIKE SHORT RODS IN PERIPHERY OF RBC'S
EHRLICHIA APPEARS AS SMALL CLUSTERS IN CYTOPLAMS
EPERY SWINE, CATTLE, LAMAS, SIMILAR LOOKING TO HAEMOBARTONELLA FELIS
ANAPLASM SMALL, DARK STAINING COCCI AT MARGIN OF RBC
CYTAUXZOON FELIS RARE CAUSE OF HEMOLYTIC ANEMIA
BABESIOSIS TEXAS TICK FEVER, REDWATER FEVER, CATTLE TICK FEVER, TEAR DROP SHAPED
MATERIALS NEEDED FOR A BLOOD SMEAR CLEAN SLIDES, BLOOD FROM A PURPLE TOP TUBE ->EDTA, CAPILLARY TUBE, SPREADER SLIDE, TRIPLE STAIN
BLOOD SMEAR FEATHERED EDGE 2/3 UP THE SLIDE
Created by: JODY84