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LAB PROCEDURES NOTES
LAB PROCEDURES 1 NOTES
| Question | Answer |
|---|---|
| RBC | ERYTHROPOIESIS |
| ERYTHROPOIESIS | ERYTHROCYTE |
| LEUKOPOIESIS | LEUKOCYTE |
| LEUKOCYTE | WHITE BLOOD CELL |
| THROMBOPOIESIS | PLATELETS |
| PLATELETS | THROMBOCYTES |
| PLASMA | SPUN DOWN GREEN TOP TUBE |
| SERUM | BLOOD CLOTS AND THEN SPUN DOWN TIGER TOP OR RED TOP TUBE |
| NEED TO CHECK TEST BEFORE PICKING | ANTICOAGULANTS |
| IF YOU DON'T USE THE BLOOD WITHIN 1 HOUR YOU SHOULD PUT THE BLOOD | IN THE REFRIDGERATOR |
| IF BLOOD IS REFRIGERATED YOU MUST | BRING THE BLOOD BACK TO ROOM TEMP BEFORE USE |
| YOU CAN'T FREEZE BLOOD BECAUSE | LYSING CELLS (BREAK APART) |
| HEPARIN | ANTICOAGULANT |
| EDTA | ETHYLENEDIAMINE TETRAOCETIC ACID |
| BLUE TOP TUBES | OXALATES & CITRATES |
| SODIUM FLOURIDE | GLUCOSE PRESERVATIVE (DIABETIC) |
| SAMPLE VOLUME | WELL HYDRATED PT WILL MAKE HALF THE AMOUNT OF PLASMA/SERUM DRAWN UP |
| CBC | COMPLETE BLOOD COUNT |
| WHICH IS BETTER MANUAL OR AUTOMATED | BOTH IS BEST |
| CBC= | TOTAL RBC, PCV, PLASMA PROTEIN CONCENTRATE (TP), TOTAL WBC, BLOOD FILM EXAM, DIFFERENTIAL WBC, RDC & WBC MORPHOLOGY, AND PLT TEST |
| READ PCV FIRST THE BREAK TUBE ABOVE | THE BUFFY COAT TO GET PLASMA |
| CLEAR TO LIGHT YELLOW | PLASMA |
| PLASMA WILL BE PINK AND CLOUDY IF THEY | JUST ATE |
| PENIA | DECREASE NUMBER OF CELLS IN THE BLOOD |
| PHILIA/CYTOSIS | INCREASES NUMBER OF CELLS IN THE BLOOD |
| LEFT SHIFT | INCREASED NUMBER OF IMMATURE NEUTROPHILS IN THE BLOOD |
| LEFT SHIFT | INCREASED NUMBER OF IMMATURE NEUTROPHILS IN THE BLOOD |
| LEUKEMIA | NEOPLASTIC CELLS IN BLOOD OR MARROW |
| LEUKEMIA | NEOPLASTIC CELLS IN BLOOD OR MARROW |
| LEUKEMOID RESPONSE | CONDITION THAT CAN BE MISTAKEN FOR LEUKOMIA |
| LEUKEMOID RESPONSE | CONDITION THAT CAN BE MISTAKEN FOR LEUKOMIA |
| NEUTROPHIL | IRREGULAR NUCLEUS-> IRREGULAR ENLONGATED WITH 3-5 LOBES |
| NEUTROPHIL | IRREGULAR NUCLEUS-> IRREGULAR ENLONGATED WITH 3-5 LOBES |
| BAND NEUTROPHIL | IMMATURE NEUTROPHIL LOOKS LIKE A HORSESHOE, |
| BAND NEUTROPHIL | IMMATURE NEUTROPHIL LOOKS LIKE A HORSESHOE, |
| BAND NEUTROPHIL | NOT COMMON IN NORMAL ANIMALS |
| BAND NEUTROPHIL | NOT COMMON IN NORMAL ANIMALS |
| LYMPHOCYTE | LARGE NUCLEUS, LIGHT BLUE CYTOPLAMS |
| LYMPHOCYTE | LARGE NUCLEUS, LIGHT BLUE CYTOPLAMS |
| LYMPHOCYTE | AGRANULAR |
| LYMPHOCYTE | AGRANULAR |
| MONOCYTE | LARGEST WBC |
| MONOCYTE | LARGEST WBC |
| MONOCYTE | VARIABLY SHAPED NUCLEI, WITH VACUOLES, AND AGRANULAR |
| MONOCYTE | VARIABLY SHAPED NUCLEI, WITH VACUOLES, AND AGRANULAR |
| EOSINOPHIL | COMMONLY STAIN RED TO PINK, MUCH LIGHTER THAN THE NUCLEUS |
| EOSINOPHIL | COMMONLY STAIN RED TO PINK, MUCH LIGHTER THAN THE NUCLEUS |
| EOSINOPHIL | TRIGGERED BY HISTAMINE RELEASE FROM MAST CELLS--ALLERGIES |
| EOSINOPHIL | TRIGGERED BY HISTAMINE RELEASE FROM MAST CELLS--ALLERGIES |
| BASOPHIL | NUCLEUS IS SIMILAR TO MONOCYTES. |
| BASOPHIL | GRANULAR |
| BASOPHIL | GRANULES STAIN DARKER THAN THE NUCLEUS DARK BUT TO BLUE BLACK. |
| BASOPHIL | TRIGGERED BY RELEASE OF EOSINOPHILS AND ALLERGIES |
| MORPHOLOGIC ABNORMALITIES SEEN IN | WBC'S |
| BIRMAN CAT NEUTROPHIL GRANULATION ANOMALLY | WILL HAVE FINE EOSINOPHILIC TO MAGENTA GRANULES |
| BIRMAN CAT NEUTROPHIL | INHERITED RECESSIVE TRAIT |
| BIRMAN CAT NEUTROPHIL | NEUTROPHIL FUNCTION IS NORMAL & AFFECTED CATS ARE HEALTHY |
| LARGE NUMBER OF BASKET CELLS = | LEUKEMIA |
| ROULEAUX | GROUP OF RBC'S IN STACKS, LOOKS LIKE STACKS OF COINS |
| ROULEAUX | COMMONLY SEEN IN HORSES |
| ROULEAUX | CAN BE SEEN IN BLOOD HELP TOO LONG OR REFRIGERATED BLOOD |
| AGGLUTINATION | OCCURS IN IMMUNE MEDIATED DISORDERS |
| AGGLUTINATION | CLUMPING OR BRIDGING OF RBC'S LOOKS LIKE A CLUSTER OF GRAPES |
| SALINE TEST | ROULEAUX WILL SEPERATE |
| SALINE TEST | AGGLUTINATION WILL NOT SEPERATE |
| ANISOCYTOSIS | VARIATION IN SIZE OF RBC'S MACROCYTES & MICROCYTES |
| POLYCHROMASIA | BLUE TINT |
| HYPOCHROMASIA | DECREASED STAINING DEFICIENT IN IRON- LOOKS LIKE CHEERIOS |
| HYPERCHROMASIA AKA SPHEROCYTES | LOOK LIKE A BALL |
| POIKILOCYTES ->ABNORMAL | NOT A Dx JUST A GENERAL STATEMENT OF RBC'S |
| SCHISTOCYES-> FRAGMENTS | DUE TO SOME TYPE OF INTRAVASCULAR TRAUMA, DIC, HEMANGIOSARCOMA |
| ACANTHROCYTES-> SPUR CELLS | ALTERED LIPID METABOLISM AND LIVER Dx |
| ECHINOCYTES | BURR CELLS |
| PREPANOCYTES-> SICKLE CELLS | BLOOD OF NORMAL DEER AND ANGORAGOALS |
| KERATOCYTES | HELMET OR BLISTER CELLS |
| SPHEROCYTES | LOOKS LIKE A BALL |
| CODOCYTES | TARGET CELLS, FOLDED CELLS |
| NUCLEATED ERYTHROVYTES | COMMONLY SEEN REGENERATIVE ANEMIA |
| BASOPHILIC STIPPLING | SMALL, DARK BLUE BODIES WITHIN RBC LEAD POISONING IN DOGS |
| HOWELL JOLLY BODIES | REMNANT SEEN IN YOUNG RBC'S |
| HEINZ BODIES | REPRESENTING DENATURED HEMOGLOBIN'S CAUSED BY CERTAIN OXIDANT DRUGS OR CHEMICALS |
| RETICULOCYTES | IMMATURE RBC'S THAT CONTAIN ORGANELLES |
| HAEMOBARTONELLA FELIS | PARASITE |
| HAEMOBARTONELLA FELIS | COMMON IN CATS |
| HAEMOBARTONELLA FELIS | AKA FELINE INFECTIOUS ANEMIA, USUALLY LOOK LIKE SHORT RODS IN PERIPHERY OF RBC'S |
| EHRLICHIA | APPEARS AS SMALL CLUSTERS IN CYTOPLAMS |
| EPERY | SWINE, CATTLE, LAMAS, SIMILAR LOOKING TO HAEMOBARTONELLA FELIS |
| ANAPLASM | SMALL, DARK STAINING COCCI AT MARGIN OF RBC |
| CYTAUXZOON FELIS | RARE CAUSE OF HEMOLYTIC ANEMIA |
| BABESIOSIS | TEXAS TICK FEVER, REDWATER FEVER, CATTLE TICK FEVER, TEAR DROP SHAPED |
| MATERIALS NEEDED FOR A BLOOD SMEAR | CLEAN SLIDES, BLOOD FROM A PURPLE TOP TUBE ->EDTA, CAPILLARY TUBE, SPREADER SLIDE, TRIPLE STAIN |
| BLOOD SMEAR | FEATHERED EDGE 2/3 UP THE SLIDE |
Created by:
JODY84
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