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Clin Lab

Clin Lab 1 Terms

normocyte/normocytic refers to normal size and mature RBCs
microcyte/microcytic RBCs with diameter less than that of a normal RBC. These cells will have a decreased MCV and can be seen with iron deficiency
Macrocyte/macrocytic RBCs with diameter greather than that of normal RBCs. Cells Larger in size than normal, Cells with have increased MCV
Anisocytoysis terms used to describe vairation in size of RBCs
Normochromic/Normochromasia A mature RBC that stains pink/red in color with an area of central pallor. Normal Staining intensity
Hypochromic/hypochromasia decreased staining intensity caused by insufficient hemoglobin within the cell
Hyperchromic/Hyperchromasia refers to cells that appear more darkly stained than normal cells. Gives cells oversatured appearance
Polychromasia terms used to describe variation in the color of RBCs
acanthocytes also called spur cells. Contained irregulary shaped margins with unevenly distributed surface projections of variable length and diameter coming from the cell wall
Echinoytes also called burr cells. spiculated cells with numerous short, evenly spaced blunt to sharp surface projections of uniform size and shape. can also appear as dark red spots on top of the cell
Codocytes also called target cells. contains central round portion of hemoglobin inside the area of central pallor.
leptocytes large, thin RBC that is folded or distorted due to increased membrane of decreased volume
Stomatocytes large, thin RBC that warps when passing through small blood vessels and creasted a more oval to elongated central pallor
spherocytes smaller RBCs that are dense and dark staining with reduced or no central pallor
dacryoctyes tear drop in shape
Elliptocytes/ovalcytes elongated or oval shaped RBCs
keratocytes also called helmet cells. cell that often has two membrane projections (horns)
torocytes contains a punched out appearance, punched out central area in the cell
schistocytes RBC Fragments suggestive of mechanical damage or vascular occlusion
Rouleaux RBCs appearing as stacks of coins or rows. Normal finding in horses, can also be seen in cats
Agglutination amorphous clumping of RBCs. can be associated with immune disorders
Reticulocyte immature RBCs released from bone marrow during periods of intense need
Types of Reticuloctyes Aggregates & punctates
Aggregate appearance clumped appearance
punctate appearance polka-dot appearance
species with anucleated cells most mammals
species with nucleated cells birds, reptiles, amphibian
normal appearance of RBCS Biconcave
Rubriblast (RBC maturation) (1) large cell with a large round nucleaus, granular chromatin, prominent nucleoli and dark blue cytoplasm
Prorubricyte (RBC maturation) (2) large cell, round nucleus with ill defined or no nucleoli, course chromatin, cytoplasm, predom. blue but may be red tinged
Rubricyte (RBC maturation) (3) smaller in size thatn prorubricyte, round nucleus with no nucleoli, nucleus contains more clumps of chromatin and cytoplasm, is predom. blue w/ increasing amount of red
Metarubricyte (RBC maturation) (4) smaller in size than rubricyte, nucleus is eccentric, small, and has dense chromatin, cytoplasm is reddish-blue
Polychromatophilic erythrocyte (RBC maturation) (5) small cell, no nucleus and cytoplasm is blue to reddish depending on maturity
mature erythrocyte (RBC maturation) (6) small cell, stains reddish-orange, biconcave discs, with central pallor
Estimated RBC count (calculation) PCV%/6 = # x 10^6 RBC/ul
Hemoglobin Estimate (calculation) PCV%/3 = # g/dl
MCV PCV%/total RBC x 10 = #fl
MCH Hgb Concentration/total RBC x 10 = #pg
MCHC Hgb concentration/PCV% x 100 = #g/dl
1/3 of all platelets are where? spleen
Function of erythrocyte? carry oxygen from lungs to rest of body's cells
decreased HCT/PCV causes what? Anemia
increased HCT/PCV causes what? dehydration and polycythemia
Polycythemia increase in amount of red blood cells in circulating blood
Why is it important to keep stain bottles tightly capped? prevents: contamination, evaporation, and PH changes
hematopoiesis production of blood cells and platelets
fluid/liquid portion of blood plasma
fluid/liquid portion of whole blood in which the fibrinogen and coagulation/clotting factors have been removed serum
Normal plasma protein values: dog/cat Dog: 6.0-7.5 g/dl Cat: 6.0-7.5 g/dl
Normal RBC cell count: dog/cat Dog: 5.0-8.5 x 10^6 RBC/ul Cat: 5.0-10.0 x 10^6 RBC/ul
main energy source fo erythrocytes glucose
Normal hemoglobin values: dog/cat dog: 12-18 g/dl cat: 8-15 g/dl
Plasma consists of ___% water? 90%
What is yielded when anticoagulated or unclotted blood sample is centrifuged? plasma
coagulated/clotted blood will yield what after centrifugation? serum
Hemoglobin function functions to help carry oxygen molecules, iron and cell metabolites throughout the body
3 plasma proteins Albumin, globulins, Fibrinogen
Globulins consists of? alpha, beta, gamma
HCT/PCV purpose? measures the precent of whole blood that is composed of rBCs
5 main parts of HCT/PCV (in order, from bottom to top) Clay plug/seal packed RBCs Buffy Coat Plasma Meniscus
Red Top Tube: Anticoagulant? Use? What does it yield? Anticoagulant: None Use: Blood Chemistry Yields: Serum
Serum Separator Tube/Marble Top: Anticoagulant? Use? What does it yield? Anticoagulant: None Use: Blood Chemistry Yields: Serum
Lavender/Purple Top: Anticoagulant? Use? What does it yield? Anticoagulant: EDTA Use: CBC Yields: Plasma
Blue Top Tube: Anticoagulant? Use? What does it yield? Anticoagulant: Sodium Citrate Use: Clotting Studies Yields: Plasma
Green Top Tube Anticoagulant? Use? What does it yield? Anticoagulant: Lithium Herparin Use: Ph levels, Lead determinants Yields: Plasma
Gray Top Tube: Anticoagulant? Use? What does it yield? Anticoagulant: potassium oxalate/sodium flouride Use: glucose determinants Yields: Plasma
Created by: 6812617



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