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1305 quiz 4
clinical laboratory quiz 4
| Question | Answer |
|---|---|
| This type of testing is used extensively in the diagnosis of viral infections | serology testing |
| what type of blood sample is required for serological testing | plasma or serum |
| continued production of antibodies after the animal is no longer ill | convalescent phase |
| a serum antibody level is also referred to as | a titer |
| protein molecules that are produced by a certain subgroup of B-lymphocytes when they are presented with a substance that's considered foreign these are produced | antibodies |
| antibodies are also known as | immunoglobulins |
| how many types of immunoglobulins are present in the body | 5 |
| what are the 5 types of immunoglobulins | IgG, IgM, IgE, IgA, IgD |
| which immunoglobulin functions to neutralize microbes and toxins | IgG |
| which immunoglobulin gives fetal and neonatal immunity by passive transfer across the placenta and through colostrum | IgG |
| which immunoglobulin constitutes 75% of serum immunoglobulins in humans | IgG |
| Which immunoglobulin is physically the largest antibody in the circulatory system that is the first to appear in response to initial exposure to an antigen | IgM |
| what helps the ability of antibodies and phagocytic cells to clear pathogens from an organism | complement system |
| which immunoglobulin is immediate in response to hypersensitivity reactions such as allergies and anaphylactic shock | IgE |
| which immunoglobulin functions to coat helminth parasites to allow destruction by eosinophils | IgE |
| which immunoglobulin is the least abundant in circulation | IgE |
| which immunoglobulin functions as mucosal immunity to protect the respiratory, intestinal, and urogenital tract | IgA |
| which immunoglobulin accumulates to 75% of the total immunoglobulins produced in the entire body | IgA |
| which immunoglobulin functions as b-lymphocyte surface antigen receptors in some species | IgD |
| which immunoglobulin makes up about 1% of proteins in the plasma membranes of immature b-lymphocytes where it is usually coexpressed with IgM | IgD |
| what are the most common immunoassays performed in the clinical practice | ELISA |
| this component of ELISA testing includes the microwell, wand, flow-through membrane, or chromatographic strip | Solid phase |
| this component of ELISA testing is the reagent that is imbedded on the solid phase and usually consists of monoclonal antibodies bound to an enzyme | conjugate |
| this component of ELISA testing is a photosensitive reagent that produces a color change in the test system | chromagen |
| when performing an ELISA test what phase is the patient sample added to | solid phase |
| when performing an ELISA test a color change will appear if what is present | an antigen |
| Rapid immunomigration assay is also known as what | immunochromatography(ICT) |
| this test is similar to ELISA testing except that gold staining is used to replace the chromagen | RIM testing/ICT |
| when performing a RIM/ICT test this component includes the chromatographic strip | solid phase |
| when performing a RIM/ICT test this component includes the antibody bound to colloidal gold or latex, instead of the enzyme | conjugate |
| when performing a RIM/ICT test this component includes a photosensitive reagent that produces a color change in the test system | Gold staining |
| this type of immunoassay doesn't require a flow solution to aid in the movement of the sample across the chromatographic strip | RIM/ICT test |
| this type of immunoassay is used for detection of antibodies to large particle antigens | agglutination testing |
| this type of immunoassay requires adding a specific antigen to the test sample | agglutination testing |
| this type of immunoassay is commonly performed on a slide | agglutination testing |
| how many types of precipitation testing are there | 3 |
| what are the three major types of precipitation testing | immunodiffusion, radioimmunodiffusion, immunoelectrophoresis |
| this type of precipitation testing is used to test for EIA, it is known as the coggins test | immunodiffusion method |
| this test requires an agar plate with wells cut into it, the wells contain the patient samples along with a positive control and a negative control | Coggins test |
| this type of precipitate testing is performed in research labs rather than in clinical settings | radioimmunoassay |
| this type of precipitate testing is similar to ELISA testing except there is a radioisotope used in place of the conjugate | radioimmunoassay |
| this type of precipitate testing detects either antigens or antibodies, instead of either or | radioimmunoassay |
| with this type of precipitate testing the amount of radiation detected equates to a volume of antigen or antibody present in the sample | radioimmunoassay |
| this type of precipitate testing is mostly performed in reference or referral labs, rather than in the clinical setting | immunoelectrophoresis |
| this type of precipitate testing is a biochemical method for separation and characterization of proteins based on electrophoresis and reaction with antibodies | immunoelectrophoresis |
| this type of serological testing is used to detect auto-antibodies(antibodies against one's own tissues) | Coombs test |
| this serological test detects IgG or IgM or complement 3 bound to the surface of RBC's | coombs test |
| this type of coombs test detects circulating autoantibodies, detects agglutination of RBC's | indirect coombs test |
| this type of coombs test is diagnostic for hemolytic disease | direct coombs test |
| this type of serological testing is not commonly performed in veterinary medicine, is used to verify a tentative diagnosis for the presence of a specific antibody or antigen found in the sample | fluorescent antibody |
Created by:
chop
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