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1305 quiz 4

clinical laboratory quiz 4

This type of testing is used extensively in the diagnosis of viral infections serology testing
what type of blood sample is required for serological testing plasma or serum
continued production of antibodies after the animal is no longer ill convalescent phase
a serum antibody level is also referred to as a titer
protein molecules that are produced by a certain subgroup of B-lymphocytes when they are presented with a substance that's considered foreign these are produced antibodies
antibodies are also known as immunoglobulins
how many types of immunoglobulins are present in the body 5
what are the 5 types of immunoglobulins IgG, IgM, IgE, IgA, IgD
which immunoglobulin functions to neutralize microbes and toxins IgG
which immunoglobulin gives fetal and neonatal immunity by passive transfer across the placenta and through colostrum IgG
which immunoglobulin constitutes 75% of serum immunoglobulins in humans IgG
Which immunoglobulin is physically the largest antibody in the circulatory system that is the first to appear in response to initial exposure to an antigen IgM
what helps the ability of antibodies and phagocytic cells to clear pathogens from an organism complement system
which immunoglobulin is immediate in response to hypersensitivity reactions such as allergies and anaphylactic shock IgE
which immunoglobulin functions to coat helminth parasites to allow destruction by eosinophils IgE
which immunoglobulin is the least abundant in circulation IgE
which immunoglobulin functions as mucosal immunity to protect the respiratory, intestinal, and urogenital tract IgA
which immunoglobulin accumulates to 75% of the total immunoglobulins produced in the entire body IgA
which immunoglobulin functions as b-lymphocyte surface antigen receptors in some species IgD
which immunoglobulin makes up about 1% of proteins in the plasma membranes of immature b-lymphocytes where it is usually coexpressed with IgM IgD
what are the most common immunoassays performed in the clinical practice ELISA
this component of ELISA testing includes the microwell, wand, flow-through membrane, or chromatographic strip Solid phase
this component of ELISA testing is the reagent that is imbedded on the solid phase and usually consists of monoclonal antibodies bound to an enzyme conjugate
this component of ELISA testing is a photosensitive reagent that produces a color change in the test system chromagen
when performing an ELISA test what phase is the patient sample added to solid phase
when performing an ELISA test a color change will appear if what is present an antigen
Rapid immunomigration assay is also known as what immunochromatography(ICT)
this test is similar to ELISA testing except that gold staining is used to replace the chromagen RIM testing/ICT
when performing a RIM/ICT test this component includes the chromatographic strip solid phase
when performing a RIM/ICT test this component includes the antibody bound to colloidal gold or latex, instead of the enzyme conjugate
when performing a RIM/ICT test this component includes a photosensitive reagent that produces a color change in the test system Gold staining
this type of immunoassay doesn't require a flow solution to aid in the movement of the sample across the chromatographic strip RIM/ICT test
this type of immunoassay is used for detection of antibodies to large particle antigens agglutination testing
this type of immunoassay requires adding a specific antigen to the test sample agglutination testing
this type of immunoassay is commonly performed on a slide agglutination testing
how many types of precipitation testing are there 3
what are the three major types of precipitation testing immunodiffusion, radioimmunodiffusion, immunoelectrophoresis
this type of precipitation testing is used to test for EIA, it is known as the coggins test immunodiffusion method
this test requires an agar plate with wells cut into it, the wells contain the patient samples along with a positive control and a negative control Coggins test
this type of precipitate testing is performed in research labs rather than in clinical settings radioimmunoassay
this type of precipitate testing is similar to ELISA testing except there is a radioisotope used in place of the conjugate radioimmunoassay
this type of precipitate testing detects either antigens or antibodies, instead of either or radioimmunoassay
with this type of precipitate testing the amount of radiation detected equates to a volume of antigen or antibody present in the sample radioimmunoassay
this type of precipitate testing is mostly performed in reference or referral labs, rather than in the clinical setting immunoelectrophoresis
this type of precipitate testing is a biochemical method for separation and characterization of proteins based on electrophoresis and reaction with antibodies immunoelectrophoresis
this type of serological testing is used to detect auto-antibodies(antibodies against one's own tissues) Coombs test
this serological test detects IgG or IgM or complement 3 bound to the surface of RBC's coombs test
this type of coombs test detects circulating autoantibodies, detects agglutination of RBC's indirect coombs test
this type of coombs test is diagnostic for hemolytic disease direct coombs test
this type of serological testing is not commonly performed in veterinary medicine, is used to verify a tentative diagnosis for the presence of a specific antibody or antigen found in the sample fluorescent antibody
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