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Cytologies
Clin Path
Question | Answer |
---|---|
What types of cells are considered to be 'exfoliative cytology'? | Those from body fluids or from the body's surface |
What is the primary purpose of cytology evaluation? | differentiate inflammation from neoplasia |
What is the evaluation of cellular architecture? | histopathology |
What is the evaluation of individual or small groups of cells? | cytology |
Why are ear cytology slides heat fixed? | melts the wax and fixes the cells |
Scrapings are useful for collection of cells from _________________. | firm lesions |
Imprints are done to collect what 2 things? | yeast and bacteria |
What is the norma flora on the body? | staph intermedius |
Is staph OR strep found in clusters? | staph |
Is staph OR strep found in chains? | strep |
What type of preparation involves preparing 4-6 slides? | Tzanch Prep |
True or false. Fine Needle biopsies are a sterile procedure. | false (Not usually) |
What size needle is used for an aspiration? | 21-25g (The smaller the better) |
What size syringe is used for an aspiration? | 3-20ml |
True or false. The smaller a syringe, the less vacuum pressure is has. | False |
What is a non-aspirate procedure? | Aspirate minus the syringe (No vacuum pressure) |
What is another name for the non-aspirate procedure? | capillary technique or stab technique |
What gauge needle is used for non-aspirate procedures? | 22g |
True or false. Non-aspirate procedures are used for small masses. | true |
What type of aspirate almost never dries completely and is diagnostic for this trait? | Lipomas |
True or false. The animal does not need to be under anesthesia for tissue biopsy collection. | False |
What should never be done before collecting a tissue biopsy? | scrubbing the lesion or disrupting the surface |
What is the preservative of choice for histopathology? | 10% formalin |
What is used to flush biopsy specimens once collected? | endoscope with sterile saline |
How are elliptic specimens commonly obtained? | scapel |
What term refers to the sample collected via a wedge biopsy that includes a cross section of the normal and the affected tissue? | transition zone |
What is the most common type of biopsy punch? | keyes cutaneous |
What are the 3 sizes available for the keyes cutaneous punch? | 4, 6, and 8mm |
How many sutures are required for a 6 or 8 mm keyes punch biopsy? | 1-2 sutures |
How many punch biopsies should be collected of various lesions? | 2-3 |
What is another name for a paracentesis? | abdominocentesis |
What is the most commonly used needle gauge when performing a centesis? | 21g |
Where is the needle inserted for a thoracocentesis? | between the 7th or 8th intercostal |
What postion must an animal be in for an abdomincentesis? | standing or lateral |
What term refers to thick, colored/cellular fluid from the body? | exudate |
What term refers to clear, thin, watery fluid that is often amber colored and from the body? | transudate |
What are the 4 methods of collecting mucus from the respiratory tract? | percutaneous, orotracheal, nasal flush, and bronchoalveolar lavage |
What type of mucus collection technique involves insertion of a needle into the trachea to infuse saline until the animal coughs? | percutaneous |
What type of mucus collection technique involves placing an ET to collect fluid via the jugular or urinary cath? | orotracheal |
What is the best technique used for collecting samples from the lower respiratory tract? | bronchoalveolar lavage |
What is the best collection technique for upper respiratory tract? | nasal flush |
What is the preferred method for bronchoalveolar lavage? | bronchoscopy |
True or false. Mucus samples should be centrifuged at a low speed. | True |
What is another name for "Squash Prep" slide technique? | compression preparation |
What slide technique produces a slide with a compression, a smear and an untouched center? | combination technique |
What type of slide technique is formed with a needle but requires a thicker layer of cells/tissue? | starfish |
What is the preferred fixative for cytology specimens? | 95% methanol |
How long should cytology slides remain in fixative? | 2-5 mins |
What type of stains are used for cytologies? | romanowsky stains |
What type of tubes should cytologic samples be collected into? | EDTA |
What type of cytologic smear has a straight abrupt edge? | line smear |
What type of cytologic smear has a feathered edge similar to a blood smear? | wedge smear |
What 3 things influence selection of cytologic smear technique? | cellularity, viscosity and homogeneity |
What are the 2 primary WBCs seen in cytologic samples involving inflammation? | neutrophils and macrophages |
What type of inflammation involves large numbers of neutrophils (>85%)? | Suppurative (Purulent) |
What type of inflammation involves more than 15% macrophages? | granulomatous |
What type of inflammation contains phagocytized material? | septic inflammation |
How many abnormal nuclear configurations are required to be identified as malignant? | 3 or more |
What type of tumors are highly cellular such as carcinomas? | epithelial cell tumors |
What type of tumors are usually less cellular and have wispy spindles such as sarcomas? | mesenchymals |
What type of tumors are mast cell tumors and histiocytomas? | round cell tumors |
What type of lymphocyte is a normal response to antigenic stimulation? | reactive lymph node |