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VT 2100 Enviroment
Standards of care for Research Animals
| Question | Answer |
|---|---|
| The Quality of Data is related to... | The quality of Animal Care |
| The focus of Facility design is | To minimize the spread of disease |
| Surgical Suites include | Prep Area Aseptic Surgery Room Recovery site |
| Requirements for Storage area | Away from Animals low temp and humidity 20cm from floor and walls |
| Describe Conventional Facilities | Single corridor w/ Rooms |
| Describe Double Corridor Facilities | Clean and Dirty Sides Personnel cannot leave threw dirty side |
| Describe Barrier Facilities | Must shower before entering Disposable clothes GERM free |
| Describe Containment Facilities | INFECTIOUS disease |
| Construction of Faclity must have | Waterproof & Seamless No Inward opening doors Now windows |
| Define Macroenviroment | Room's Temp, Humidity, & Light |
| Define Microenvioroment | Primary Enclosure |
| Rodents Perfer a ______ Temp | Higher than Bunnies |
| Primates Perfer a ______ Temp | Higher cuz tropical |
| TNZ stands for | Thermoneutral Zone No need to cool or heat body |
| Optimal Temp for animals is... | Slightly below TNZ |
| Most animals like __% - __% Humidity | 45%-50% |
| Rodents Prefer __% - __% Humidity | 40%-70% |
| Dogs, Cats, & monkeys Prefer __% - __% Humidity | 30%-70% |
| Ventilation requires ___ air exchangers per hour | 10-15 |
| Positive air Pressure moves... | Room -> outside |
| Positive Air Pressure keeps contaminates In/Out? | Out Ex. Surgery |
| Negative Air Pressure moves... | Outside -> Room |
| Negative air pressure keeps contaminates In/Out? | In Ex- Infectious rooms |
| Define Diurnal | 12 hours Light 12 hours Night |
| Define Pollyesturs | Light affects Breeding |
| Requirements of Primary enclosure | Contained w/o restriant Observation w/o disterbence |
| Polycarbonate Plastic is... | Transparent |
| Polypropylene plastic is... | Opaque |
| Polystyrene plastic is... | Disposable |
| Define Show Box Cage | Solid Bottom w/ grid top |
| Define Suspended cage | Mesh/grid bottom |
| Define metabolic Cage | collects pee&Poo Food outside |
| Define Gang Cage | Multiple Food and H20 areas houses groups |
| Define Purpose bred | Bred For Research Class A |
| Define Random Source | Shelter animals Class B |
| Minimal Quarantine for all animals | 48 hours |
| Define Gnotobiology | Study of animals with Flora & Fauna |
| Define Conventional Gnotobiology | Unkown microflora |
| Define Gnotobiotics | Well Defined microflora |
| Define SPF | Specific Pathogen Free |
| Define Axenic | Germ Free |
| Axenic Animals require | Sterile food Dietary Supplements |
| Axenic Animals Have... | Thinner walled GI and Lg lumen Grow Quicker |
| Define Csaren Derived | Sterial isolation Uterus removed |
| Define Barrier-Sustained | C-section maintained sterile Controlled living enviroment |
| Define Sential | Suspectable to pathogens |
| Define Biosaftey level I | Not contagious to humans |
| Define Biosafey Level II | Causes DZ if handled impoperly (MM, ingestion, Puncture) |
| Define Biosaftey Level III | Serious disease |
| Biosafety Level IV | Life threatening |
| Disposal of Biosaftey level I | None indicated |