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ANSC 351
Exam 1
| Question | Answer |
|---|---|
| Meat is defined as? | as those animal tissue that are suitable for use as food |
| Kinds of meat? | Carcass/fresh meat, variety meats, reclaimed meat, processed meat, cell cultured meat |
| What is Carcass/ Fresh meat | Eviscerated body of domestic mammals (Bone, connective tissue, muscle, fat) |
| What are variety meats | Edible organs and glands (kidney, liver, heart, etc) |
| What is reclaimed meat | Products from bone, fat, or blood |
| Mechanically separated meat from the bone comes from? | pork and chicken |
| Lean finely textured beef is from | fat |
| Plasma protein isolate | blood |
| What is processed meat | Frankfurters and Hams |
| What is cell cultured meat | product that mimics the structure, composition, and nutritional value of regular meat product but is generated from animal stem cells |
| Explain skeletal muscle | voluntary, striated, responsible for body movements, multinucleated, 35-65% of carcass weight |
| Explain cardiac muscle | involuntary, striated, unique to the heart, single nucleated, numerous glycogen granules and mitochondria |
| Explain Smooth muscle | involuntary, unstriated, single nucleated, mainly contained in blood vessels, GI tract, and repro tract |
| How many muscles do mammals have roughly | 600 |
| Name the three layers of connective tissues that skeletal muscle has and what they do | Epimysium, perimysium, endomysium, they enclose it, provide structure and compartmentalize the muscle fibers within the muscle |
| What is the sarcolemma | cell membrane; composed of protein and lipid; relatively elastic; forms transverse tubules |
| What is the sarcoplasmic reticulum | storage site of Ca2+in resting muscle |
| T tubules are | Triad- 2 terminal cisterna and 1 t tubule; contraction signal travels through t tubule |
| NMJ | At sarcolemma; motor nerve endings terminate here |
| sarcoplasm | same as cytoplasm; organelles suspended, 75-80% water; lipids, glycogen granules, ribosomes, etc |
| mitochondria is | powerhouse of the cell, provides cell with a source of chemical energy |
| lysosomes are | contains proteolytic enzymes known as cathepsins |
| myofibrils are | unique to muscle tissue; long rod 1 to 2 um in diameter; bathed by sarcoplasm and extend the entire length of the muscle fiber |
| what are banding areas | myofilaments are contained within the myofibrils and are the contractile component |
| What is the I band | isotropic and singly refractive in polarized light |
| what is the A band | doubly refractive and anisotropic in polarized light |
| z line is | what bisects the I band |
| what is a sarcomere | unit length between two z lines |
| what is h zone | center of A band (myosin only) |
| what is m line | bisects the center of A band |
| Pseudo H zone | narrow region within H zone (myosin tail only) |
| What are the myofibrillar proteins | contractile, regulatory, and structural |
| Contractile | Actin and myosin |
| regulatory | tropomyosin and troponin |
| structural | nebulin and titin |
| meat protein that is 30% | sarcoplasmic proteins |
| meat protein that is 15% | stromal proteins |
| What is myosin | 45% of myofibrillar protein; double headed |
| the two binding sites of myosin | actin binding site, atp binding site |
| What is actin | about 20% of the myofibrillar protein; G actin and F actin |
| Tropomyosin | 5% of myofibrillar protein; covers the active binding site of actin on F actin |
| Troponin | 5% of myofibrillar protein; molecular switch-regulates the calcium dependent activation of muscle contraction |
| TnI | Inhibitor |
| TnC | Calcium |
| TnT | Troponin binding complex |
| Titin | 10% of myofibrillar protein; most abundant structural protein and largest; scarffolds for thick filaments; I band elastic; A band inelastic |
| Nebulin | 4% of myofibrillar protein; aligns thin filaments |
| where does nerve stimulus start | brain (CNS) |
| where does nerve stimulus arrive | transmitted to muscle via nerve fibers (PNS) |
| what is a motor unit | number of muscle fibers innervated by a single motor neuron |
| what is a motor end plate | transfers the electrical impulse from the nerve ending to the muscle fiber membrane |
| what is the nmj | action potential transferred from nerve fiber to muscle fiber at nmj |
| excitation contraction coupling is | process by which a muscular action potential in the muscle fiber causes the myofibrils to contract |
| what is acetylcholinesterase | secreted by the muscle cell into the synaptic cleft breaking down ACh to acetic acid and choline |
| What happens to choline | reabsorbed by the presynaptic terminal and combined with acetic acid to form ACh, which enters the synaptic vesicles |
| what is an action potential | Na+ permeability increases to higher value than K+ ions, high rate of Na+ diffusion into cell-depolarization |
| where does an action potential travel | from nmj and progresses longitudinally in both directions along sarcolemma |
| what do t tubules do with action potentials | transmit stimuli to the interior of the fiber; the traid it is transferred to the SR |
| what does the Sr do with action potential | Ca2+ to be released into the sarcoplasmic fluid |
| step 1 on sliding filament theory | cross bridge formation |
| step 2 on sliding filament theory | power stroke |
| step 3 on sliding filament theory | cross bridge detachment |
| step 4 on sliding filament theory | reactivation of myosin head |
| how to reestablish resting state | high ATP and low Ca2+, repolarization, Ca2+ pump active (requires ATP) |
| what are muscle fiber types determined by | contraction, rate, color, metabolic differences |
| type 1 | red fiber (beta red), slow contraction, oxidative, high lipid, small diameter, myoglobin |
| Type 2A | Red fiber (alpha red), intermediate, fast contraction, anaerobic, aerobic |
| Type 2X | White fiber (beta white), fast contraction |
| Type 2B | White fiber (alpha white), fast contraction, larger fiber, glycolytic, anaerobic, fewer mitochondria, lipid, and myoglobin |
| ATP is the ultimate source of energy for | contractile process, pumping calcium back, maintaining Na/K pump |
| Phosphocreatine | most immediate source of energy, short use only, 1 |
| Aerobic metabolism | most efficient source of atp production, cellular respiration, 37 atp from 1 glycogen molecule |
| anaerobic metabolism | short term supply of energy, 3, lactic acid, lowering pH |
| After slaughter | no more blood circulation, no more oxygen supply, no more circulatory system, anaerobic pathway to maintain homeostasis |
| what is homeostasis | maintenance of a physiologically balanced internal environment in response to changes in external conditions |
| what is immobilization | ensures the animal in unconscious and insensible to pain before bled out at slaughter |
| what is exsanguination | complete circulatory system failures to muscles |
| what happens to oxygen postmortem | depletion, anaerobic, increase in lactic acid, pH decline |
| what does the rate and extent of pH decline depend on | amount of glycogen |
| rigor mortis | muscle stiffness, permanent cross bridge formation, relaxation not available |
| delay phase | time during which the muscle is extensible and elastic, atp still available, creatin phosphate being used up, glycolysis going on, using oxygen |
| onset phase | muscle begins to lose its extensibility, glycogen is decreasing, atp decreasing, lactic acid increasing, pH declining, actin/myosin bridges begin to form |
| completion phase | ATP can no longer be formed, CP gone, glycogen very low, atp very low, pH drop is almost completed, lactic acid very high, muscles become inextensible |
| resolution phase | softening of rigor, protein degredation of z disks, tenderness development initiated |
| pH decline and rigor development muscle to muscle variation | fish, chicken, turkey, pork, lamb, beef |