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RATS Quiz 4
Lecture 7: Mice Part 2
| Question | Answer |
|---|---|
| blood collection | up to 10% of circulating blood volume can be withdrawn every 2-4 weeks, more is possible if fluid replacement is provided |
| blood collection sites | facial vein, retro-orbital sinus, lateral saphenous vein, submental region, cardiac puncture |
| genotyping | used to characterize the genetic makeup of breeding |
| where is the sample of DNA taken for genotyping? | taken from the distal tip of the tail, or an ear punch tissue can also be used |
| urine collection | mice can be stimulated to urinate upon handling or placement on a cold surface, use of metabolic cage |
| drug administration | obtain accurate body weight, dilute drugs, use syringes designed for small volume administration, calculate dosing volumes on a mL/kg basis |
| oral administration | drug mixed in feed or water, specialized gavage needles, use appropriate volume when giving by syringe or gavage |
| injectable drug administration | smaller needles (25G) should be used, SQ under loose skin over shoulders |
| SQ | subcutaneous, loose skin around neck or abdomen |
| IV | intravenous; saphenous, jugular, femoral, lateral saphenous |
| IM | intramuscular; quadriceps, gluteal, triceps |
| IP | intraperitoneal; lower right quadrant of the abdomen, avoid organs like the spleen |
| which route of injection is best for irritating ketamine and other irritating drugs? | intraperitoneal (IP) |
| a combination of what drugs is a good injectable regiment for surgical procedures? | ketamine and xylazine |
| what inhalants can be used with a precision vaporizer? | isoflurane, sevoflurane |
| intra and post-operative care | prevention of HYPOTHERMIA is key, use sterile ophthalmic ointment is animal's blink reflex is lost, provide SQ fluids to animals who have undergone prolonged surgeries to prevent dehydration |
| pain control | analgesics must be administered to control surgical pain |
| sings of pain | difficult of assess; decreased food and water intake, decrease in activity, piloerection |
| NSAIDs used | meloxicam, ketoprofen, carprofen |
| opioids used | butorphanol, buprenorphine |
| post-operative care | should be placed on a dry paper towel, should not be placed with animals that are not anesthetized until they are fully awake, circulating water blanket/heat lamp to prevent hypothermia, provision of moistened chow/gelatinous water can assist in recovery |
Created by:
mkroon26
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