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Clin Path Quiz 6/7
Lecture 7: Manual WBC Counts
| Question | Answer |
|---|---|
| what is a white blood cell? | a cell that acts as a part of the immune response and travels through the blood |
| 5 different types of WBCs: | neutrophils, lymphocytes, monocytes, eosinophils, basophils |
| purpose of a WBC count: | to determine the number of leukocytes in the circulating blood |
| normal canine WBC count: | 6000-17000/uL |
| normal feline WBC count: | 5500-19500/uL |
| -penia: | decreased number of cells in the blood (leukopenia, neutropenia, etc.) |
| -philia or -cytosis: | increased number of cells in the blood (leukocytosis, neutrophilia, etc.) |
| leukocytosis: | increased WBC count |
| causes of leukocytosis: | infection, inflammation, stress, steroids |
| leukopenia: | decreased WBC count |
| causes of leukopenia: | overwhelming disease, problem with production in the bone marrow |
| how to measure a WBC count: | manually (rarely used in practice) or automated (rapid results, but errors may occur if abnormal cells are present) |
| nucleated erythrocytes (nRBCs): | presences of these indicates the release of immature erythrocytes into circulation (aka metarubicytes); can falsely elevated the automated leukocyte count |
| calculation of corrected WBC/uL: | for every 100 leukocytes, determine how many nRBCs are rpresent |
| methods for manual WBC count: | hematology pipettes (not used due to easier methods being available) and Leuko-Tic Bioanalytic System |
| Leuko-Tic Bioanalytic System: | ready to use test kit that uses a 1:20 dilution |
| what is used to actually count WBCs? | hemocytometer (same one used to count RBCs) |
| in what section of the grid do you count WBCs? | the 4x4 sections of the hemocytometer, count of 10x magnification |
| 1 cubic centimeter (cm3) equals: | 1 mL (1 cc = 1 mL) |
| 1 cubic millimeter (mm3) equals: | 1 microliter (uL) |
| 1 mL equals: | 1000 uL |
| what is the volume factor? | the grid in which WBCs are counted is 1mm long by 1mm wide by .1mm DEEP; you are counting all the WBCs in .4 uL of blood |
| calculation for total WBC count: | (WBCs counted x 20)/.4 uL = WBC counted x 50 = WBCs/uL |
| sources for error when performing a WBC count: | incorrect technique when pipetting, incorrect amounts of blood and reagent, double counting cells, not mixing well enough to lyse RBCs, charging hemocytometer incorrectly |
| if cells fall on a grid line, count only the cells are are: | on the TOP line or on the LEFT line |
| downside of the WBC count: | the total WBC count does not tell you which leukocytes are increased, decreased or normal |
| resolution to the downside: | differential WBC count |
Created by:
mkroon26
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