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Gas Chromatography
Food Analysis Final Exam
| What are the sample requirements for GC? | Sample must be volatile and can be polar/nonpolar. |
| Separations in GC occur by _____. | partitioning |
| T or F: Retention time is unique to the compound of interest. | False |
| ___ ___ is used to identify a sample component. | Retention time |
| ___ ___ is a parameter used to measure the quantity of the sample. | Peak area |
| Give three advantages of GC. | High resolution separation, fast analysis, robust and reliable. |
| What is the purpose of the detector in GC? | Recognizes and responds to sample components as they elute from the column. |
| What are the three heated zones in GC instrumentation? | Inlet, column oven, detector |
| Why is the detector heated in GC? | To keep it clean. |
| What is the most common mode for GC? | Split injections |
| What is the equation for split ratio? | Split ratio = (split + column flow)/column flow |
| A splitless chromatogram is used for ____ analysis. | trace |
| Give three symptoms of a dirty capillary liner. | Extraneous peaks, peak tailing of active compounds, loss of active compounds. |
| The ideal resolution (separation) is a function of what three factors? | Capacity, selectivity, and efficiency. |
| Define GC detector. | A device which senses the presence of a component different from the carrier gas, and converts that information to an electrical signal. |
| What are the four components of a mass spectrometer? | Ion source, mass analyzer, detector, and vacuum system. |
| Retention times with GC/MS are considered ____ data. | qualitative |
| The size of a peak determines what in GC/MS? | How much is present |
| Name the three different approaches for quantification with GC/MS. | External standard calibration, internal standard calibration, and area %. |
| T or F: Three level calibration is a minimum requirement to provide evidence of the linearity of a calibration curve. | True |
| Name three requirements for a good internal standard. | The compound is not present in the sample, the compound is stable, the compound is well separated from other sample components, similar properties to analyte. |
| What is the purpose of solid phase extraction? | Pre-separation (eg. eliminate matrix) |
| Describe SPE. | Sample components are separated based on affinity for stationary/mobile phase. |
| What are the three steps in solid phase micro extraction? | Sample preparation, extraction, and desorption. |
| T or F: The entire sample is analyzed in headspace GC. | False |
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goberoi
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