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Mass Spectrometry
Food Analysis Final Exam
| T or F: Mass spectrometry is suitable for both qualitative and quantitative analysis. | True |
| Mass spectrometry relies on the formation of ____ to give a mass over charge ratio. | ions |
| Define fragmentation. | Smash a molecule to pieces in order to determine what the molecule was. |
| What is the purpose of the ion source in mass spectrometry? | Converts neutral organic compounds into ions. |
| What is the purpose of the ion analyzer in mass spectrometry? | Separates ions according to their mass to charge ratio. |
| What is the purpose of the ion or mass detector in mass spectrometry? | Generates a signal when receiving an ion - signals appear as peaks on a spectrum. |
| Define base peak. | The peak with the highest abundance |
| T or F: The sample need not be thermally volatile and stable for use with electron impact ionization. | False |
| What type of compounds can be used with electrospray ionization? | charged, polar, or basic |
| _____ can be used in combination with electron impact ionization. | Gas chromatography |
| _____ can be used in combination with electrospray ionization. | HPLC |
| T or F: Electron impact ionization is more harsh and electrospray ionization. | True |
| What type of compounds can be used with atmospheric pressure chemical ionization? | uncharged, non-basic, or low-polarity |
| Describe selected ion monitoring. | Votlage corresponds to m/z ratio and a mass scan is completed - allowing a particular ion to flow through the quadrupole ion filter depending on voltage used. |
| Name three advantages of the quadrupole method. | Good reproducability, small/compact equipment, low-cost, low resolution. |
| An electromagnetic field of given frequencies can trap ions of certain ____ range. | m/z |
| Describe how the ion trap works. | The ion trap is set to a specific m/z range to isolate ion if interest, energy is pulsed through and breaks it up, then the subsequent ions are measured. |
| If you wanted to structurally identify a compound, a ____ ion trap would be the first choice. | quadrupole |
| If you wanted to quantitatively analyze a compound, a ____ or ____ ion trap would be the first choice. | linear, triple quadrupole |
| _____ is a useful machine for proteomics. | Q-TOF |
| _____ is a useful machine in metabolomics. | C-TOF |
| What happens during fragmentation of alkanes? | Fragmentation occurs in the middle of the unbranched chains rather than at the ends. |
| Describe three mass spectrometry applications. | Detect/identify steroids in athletes, monitor breath of patients during surgery, determine whether a food item is adulterated, detect toxins in fish. |
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