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FoodMicroLecture 16

Food Micro Midterm 3

Define a surrogate microorganism. A non-pathogenic species and strain responding to a particular treatment in a manner equivalent to a pathogenic species and strain.
Why is using a surrogate microorganism advantageous? They allow for biological verification of the treatment without introducing pathogens into a food processing area.
What are the two types of food safety challenge studies with surrogate organisms? Growth inhibition studies, pathogen inactivation studies.
What are surrogate organisms used to validate? Processing procedures, product storage conditions, shelf-life.
When are surrogates necessary? In-plant validations studies
What are three things which surrogates cannot do? Persist in processing environment, become a spoilage microorganism, gain virulence
The number of surrogates ideally should _____ with those of the pathogen of concern. correlate
The surrogate should exist in _____ numbers than the pathogen during the existence. higher
Surrogates based on pathogen of concern are implicated in _____ or _____. risk assessment, prior history
What are three desirable characteristics of surrogate organisms? Easy to differentiate from background microflora, genetically stable, easily enumerated
Define a cocktail. A mixture of a number of individual strains of surrogates.
What is the advantage to cocktails? Reduces the possibility of choosing the wrong strain as the 'most appropriate strain'.
What is the disadvantage of cocktails? It is difficult to see subtle differences between individual strains.
What is the difference between pathogenicity and virulence? Pathogenicity refers to the ability of a microorganism to cause illness, while virus refers to the degree to which illness is caused.
Define a BSL 2 organism. Microorganisms which pose moderate potential hazard to personnel and the environment.
Give an example of a BSL 2 organism. Salmonella
Define a BSL 1 organism. Microorganisms which pose no evidence for safety concerns as a surrogate.
Name an example of a bacterial spore surrogate. C. sporogenes
T or F: Surrogates for one process may not apply to another process. True
T or F: C. sporogenes can be used to predict botulinum toxin production. False
What organism is used as a surrogate for Salmonella? E. faecium
Created by: goberoi



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