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RBC Morphology

QuestionAnswer
Under what magnification are microfilariae, phagocytized organisms, atypical cells, and platelet clumping first assessed under? Low magnification (10x or 20x)
Under what magnification is RBC morphology, WBC morphology, and Platelet morphology assessed? Oil-immersion (100x)
Erythrocytes that are stacked as if in a row like coins is called what? rouleaux
In what species is rouleaux normally found? feline and equine
The irregular clumping of RBC's is called what? agglutination
What kind of anemia frequently has agglutination of the erythrocytes? IMHA (Immune Mediated Hemolytic Anemia)
What does metarubricytosis mean? increased nucleated RBC's in blood
When an animal is first diagnosed with an anemia the first thing that needs to be decided is what? Is it regenerative or non-regenerative?
What is the normal measurement of a canine RBC? 7 microns
What distinguishes a canine RBC from a feline RBC? central pallor is more pronounced in canine and canine are larger
Where should RBC morphology be looked at on a blood film? monolayer
Abnormal RBC shapes is called what? poikilocytosis
Dirofilaria immitis microfilaria can be found in what area of a blood film usually? feathered edge
If RBCs have a pale color and the central pallor is enlarged what would that be called? hypochromic RBC
If the MCV is increased what could be causing this increase? macrocytosis due to a regenerative anemia
Acanthocytes are usually seen in dogs with spleen or liver disorders. What do acanthocytes look like? RBC with irregular unevenly spaced projections
What is another name for codocyte? target cell, mexican hat cell
Codocytes are common in what type of anemia? regenerative
What is the reason for the RBC to form a"target"? Because there is more RBC membrane than Hgb inside so it forms a bulge in the center of the cell.
A dacryocyte is shaped how? tear drop
If the dacryocytes are all pointing in the same direction is this a pathological problem or an artifact and why? artifact due to smear preparation
What is the difference in appearance between echinocytes and acanthocytes? echinocytes have equally spaced projections and acanthocytes are irregularly spaced
If a dog is bitten by a rattlesnake what RBC morphology could be seen? echinocytes
Crenation is also called what? artifactual echinocytosis
What causes crenation? not drying blood film fast enough and exposure to EDTA
Elliptocytes are normally found in what species? camelids, avians, reptiles, and amphibians
Schistocytes are formed due to what? Fibrin strands in vessels that traumatize the RBCs as they flow through
Schistocytes are commonly seen in what disease situation? DIC (disseminated intravascular coagulation)
The word part kerato means what? horn
A keratocyte looks like a RBC with what? horns
A RBC that contains the same volume of Hgb but appears on blood smear to be small and have no central pallor and are commonly seen in IMHA is called what? spherocyte
A RBC that has a mouth like central pallor is called what? stomatocyte
What can cause stomatocytes? hereditary defect in some breeds or as an artifact
Another name for punched out cell is what? Torocyte
What do we as technicians have to differentiate when we see a torocyte to be sure it isn't this? hypochromic cells
Deer breeds are known to have this shape of RBC. drepanocytes (sickle-shaped)
Describe what a polychromatic RBC would look like. larger than normal, bluish
Polychromasia is a sign of what type of anemia? regenerative
If there is not any polychromasia, no nRBC's, no anisocytosis and the dog has a PCV of 18%; is this regenerative or non-regenerative anemia? non-regenerative
What stain is used to stain for reticulocytes? New Methylene Blue
If polychromatophils are seen in a blood film what should be the next step to perform with the blood sample to see if the anemia is regenerative or non-regenerative? reticulocyte stain with New Methylene Blue
Which type of reticulocyte do we count in cats when performing a reticulocyte count? aggregate
What are the two types of reticulocytes seen in cats? punctate and aggregate
When staining with NMB stain for reticulocytes what is it that the stain is adhering to for us to be able to see the reticulocytes? RNA
When you see reticulocytes in numbers greater than 60,000 microliters in cats is that a good sign or bad sign? good
When you see reticulocytes in numbers greater than 80,000 microliters in dogs is that a good sign or bad sign? good
Would it be necessary to perform a NMB stain on an equine blood film to assess anemia? No. They normally are not present in the equine blood.
When a ruminant has a regenerative anemia these inclusions may be seen on the erythrocyte. basophilic stippling
If a dog is poisoned with lead these inclusion bodies may be seen on the RBC. basophilic stippling
This inclusion body protrudes from the surface of the erythrocyte and can appear as slightly pale when using Wright's stain. Heinz bodies
Heinz bodies can be seen as pale blue, protruding, rounded structures when NMB stain is used to stain the blood film. True or False True
What are Heinz bodies composed of? denatured Hgb caused by oxidative damage
What species can Heinz bodies be normally found? felines
This inclusion is a nuclear remnant found on the surface of the RBC. Howell-Jolly body
Created by: spoitevint