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AgEny L5 DNA
structure of DNA
Question | Answer |
---|---|
Chargaffs rules | Adenine pairs with thymine. guanine pairs with cytosine |
Rosalind Franklin | studied the structure of DNA using xrays. she discovered that DNA is a helix and that it has repeating patterns |
Watson and Crick | DNA is a polymer of nucleic acids with a sugar phosphate backbone on the outside and base pairs on the inside |
purines are | adenine and guanine |
pyramidines are | thymine and cytosine( keep the ys together) |
there are two hydrogen bonds between | adenine and thymine |
there are three hydrogen bonds between | guanine and cytosine |
number of base pairs per turn of DNA | 10 |
antiparallel means | that one chain goes upwards and the other chain goes downwards. the chain is read from 5'to 3' |
a gene is determined by | the order of bases along a segment |
genetic material must be | able to replicate, under go variation and evolve |
replication in DNA is described as | semiconservative ...one of the original strands are in each new strand |
enzyme which unwinds DNA | Helicase |
enzyme which attaches a primer to commence replication | RNA Polymerase |
Enzyme which attaches nucleotides | DNA Polymerase |
Steps in replication | the parent molecule has two complementary strands, the two strands are separated by enzymes, each old strand acts as a template for a new strand |
cellular and biochemical processes are controlled by | genes |
genes code for | a polypeptide |
lysozyme consists of | a single polypeptide chain |
haemoglobin consists of | several polypeptide chains |
there are two stages in protein synthes | transcription and translation |
transcription occurs in the nucleus | RNA polymerase makes mRNA |
TRanslation occurs in the cytoplasm/ribosome | a polypeptide chain is made using the information on mRNA |
every 3 bases on mRNA is called | a codon |
tRNA carrys in( this is the anticodon) | an amino acid to mRNA |
mRNA is processed before it | leaves the nucleus |
a cap attached to the 5' end of mRNA | helps the ribosome to attach to it |
a poly-A-tail is attached to the 3' end | this helps mRNA out of the nucleus and prevents breakdown by enzymes |
introns are removed by | enzymes |
anino acids join to form | a polypeptide |
number of commonly occurring amino acids | 20 |
several codons can code for | 1 amino acid |
GAA and GAG | glutamic acid |
a mutation is | a change in the genetic material of an organism |
a point mutation is | a change involving one or a few base pairs |
if mutations occur on the introns | they are not expressed |
mutations can be | substitution SNP = single nucleotide polymorphism , insertions or deletions |
replacements involve | swapping one nucleotide for another |
insertions or deletions...indels | addition or loss of one or more nucleotide pairs, all codons after frameshift cannot be read especially if this does not occur in . multiples of 3. Acridine |
silent mutations occur when | the new base sequence also codes for the same amino acid. UAA<UGA<UAG all = stop |
missense mutation | new base sequence does not code for the same amino acid, if new AA similar to original one change might not be noticed |
example of missense mutation | sickle cell anaemia |
nonsense mutations | a substitution changes an amino acid codon to a stop codon |
mutations in DNA caused by | xrays, radon, uv light, chemicals including AZT,nitrous acid, and acridine |
viral polymerase uses AZT instead of the base | thymine |
a kilo base pair | one thousand pairs |
mega base pair | million base pairs |
giga base pair | billion base pairs |
tera base pair | trillion base pairs |
Analysis of SNPs(genetic markers) | used for genomic selection programme in dairy and beef cattle |