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AgEny L5 DNA

structure of DNA

QuestionAnswer
Chargaffs rules Adenine pairs with thymine. guanine pairs with cytosine
Rosalind Franklin studied the structure of DNA using xrays. she discovered that DNA is a helix and that it has repeating patterns
Watson and Crick DNA is a polymer of nucleic acids with a sugar phosphate backbone on the outside and base pairs on the inside
purines are adenine and guanine
pyramidines are thymine and cytosine( keep the ys together)
there are two hydrogen bonds between adenine and thymine
there are three hydrogen bonds between guanine and cytosine
number of base pairs per turn of DNA 10
antiparallel means that one chain goes upwards and the other chain goes downwards. the chain is read from 5'to 3'
a gene is determined by the order of bases along a segment
genetic material must be able to replicate, under go variation and evolve
replication in DNA is described as semiconservative ...one of the original strands are in each new strand
enzyme which unwinds DNA Helicase
enzyme which attaches a primer to commence replication RNA Polymerase
Enzyme which attaches nucleotides DNA Polymerase
Steps in replication the parent molecule has two complementary strands, the two strands are separated by enzymes, each old strand acts as a template for a new strand
cellular and biochemical processes are controlled by genes
genes code for a polypeptide
lysozyme consists of a single polypeptide chain
haemoglobin consists of several polypeptide chains
there are two stages in protein synthes transcription and translation
transcription occurs in the nucleus RNA polymerase makes mRNA
TRanslation occurs in the cytoplasm/ribosome a polypeptide chain is made using the information on mRNA
every 3 bases on mRNA is called a codon
tRNA carrys in( this is the anticodon) an amino acid to mRNA
mRNA is processed before it leaves the nucleus
a cap attached to the 5' end of mRNA helps the ribosome to attach to it
a poly-A-tail is attached to the 3' end this helps mRNA out of the nucleus and prevents breakdown by enzymes
introns are removed by enzymes
anino acids join to form a polypeptide
number of commonly occurring amino acids 20
several codons can code for 1 amino acid
GAA and GAG glutamic acid
a mutation is a change in the genetic material of an organism
a point mutation is a change involving one or a few base pairs
if mutations occur on the introns they are not expressed
mutations can be substitution SNP = single nucleotide polymorphism , insertions or deletions
replacements involve swapping one nucleotide for another
insertions or deletions...indels addition or loss of one or more nucleotide pairs, all codons after frameshift cannot be read especially if this does not occur in . multiples of 3. Acridine
silent mutations occur when the new base sequence also codes for the same amino acid. UAA<UGA<UAG all = stop
missense mutation new base sequence does not code for the same amino acid, if new AA similar to original one change might not be noticed
example of missense mutation sickle cell anaemia
nonsense mutations a substitution changes an amino acid codon to a stop codon
mutations in DNA caused by xrays, radon, uv light, chemicals including AZT,nitrous acid, and acridine
viral polymerase uses AZT instead of the base thymine
a kilo base pair one thousand pairs
mega base pair million base pairs
giga base pair billion base pairs
tera base pair trillion base pairs
Analysis of SNPs(genetic markers) used for genomic selection programme in dairy and beef cattle
Created by: MKHealy
Popular Agriculture sets

 

 



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