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LAT Certification
Chapter 8
Question | Answer |
---|---|
What are chromosomes? | Long coiled strands of DNA |
What are alleles? | Different versions of the same genes |
What is genotyping? | Genetic testing |
What is a homozygote? | When both genes of a pair are the same |
What is a heterozygote? | When the genes of a pair are different |
What is a wild type? | When an animal does not contain the allele of interest |
If a gene is referred to as null, what is this? | When a gene is absent or turned off due to a mutation that block's the gene's expression |
What is homozygous null? | Both copies of the gene are absent or turned off-gene produces no product and the animal is called a knockout |
What is genetic engineering? | The science of manipulating the genetic makeup of a living organism |
What are the types of targeted mutation? How do they differ? | Knockin-new genes are inserted Knockout-targeted mutation blocks the function of the gene |
What is pronuclear injection? | Technique performed on a fertilized egg in a single-cell stage. Genes are injected with a tiny glass pipette directly into the cell. DNA is incorporated randomly into the chromosomes as they divide and the embryo develops |
What is homologous recombination? | Embryonic stem cells are collected and used to produce embryonic stem (ES) cell lines-DNA is inserted into these cells by electroporation. Cells containing the mutated gene are then injected into another developing blastocyst |
What is a chimera? | An animal with some of its cells containing the new gene and some without the new gene |
What is the most common technique to determine genotype? | Polymerase chain reaction (PCR) |
Describe the PCR process | A sample of tissue is taken, the tissue is dissolved and the mix is placed in a tube with an enzyme and short DNA fragments of the gene of interest, placed into PCR machine, which takes samples through cycles of heating and cooling, then amplification |
What is gel electrophoresis? | How to visualize an amplified gene-electric current passed through gel, which separates the DNA into a column, can determine if an animal possesses the gene by where the bands accumulate |
What is a phenotype? | How genes express traits; the measurable result of the genes and how they interact with the environment |
What are the different categories of phenotypes? | Visual, behavioral, demonstrable, inducible, physiological |
What are linked genes? | Genes that are located adjacent to one another on the same chromosome, and tend to be inherited together. |
What is a mating system? | The method used to pair animals for mating |
What is monogamous mating? | One female is bred with one male |
What is polygamous mating? | Two or more females are bred with one male; females are removed from mating cage once they are determined to be pregnant |
What is harem mating? | Two or more females are bred with one male; the group is kept together throughout pregnancies |
What is intensive breeding? | When the male remains with the female or females continuously |
What are some advantages to intensive breeding? | number of litters per female is maximized per unit of time, postpartum estrus, labor requirements are lower as animals are not removed from cage, recordkeeping is simplified |
What are some disadvantages to intensive breeding? | Higher demand for space, necessitates more males in the colony, older litter must be weaned before the next litter is born to prevent overcrowding |
What is nonintensive breeding? | When the stud male and females are housed separately while the females are rearing their young. The female will not mate again until the young are weaned |
What are the advantages to nonintensive breeding? | Reduces the fighting between aggressive females, newborns cannot be killed by the male, there is greater control in timing litters |
What are the disadvantages to nonintensive breeding? | labor costs may be higher as animals will have to be moved in and out of cages, no postpartum estrus, more care in recordkeeping to identify poor producers |
Why is a timed mating system used? | To provide embryos or newborn mice at a precise date needed for experiments |
What is the Whitten effect? | Initiating estrus in group housed females by adding a male to the cage |
What is a breeding scheme? | The plan for producing a colony with a desired genetic makeup |
What is outbreeding? | Breeding animals that are unrelated or only distantly related |
Why are outbred stocks bred? | To maintain the genetic difference among the animals. Scheme often produces heartier offspring and larger litter sizes |
What is inbreeding? | Producing animals with minimal genetic variation by brother-sister matings |
What are the 3 distinct colonies used to establish an inbreeding colony? | Foundation, Expansion, Production |
What is the foundation colony? | The original animals with the desired DNA |
What is the expansion colony? | Expansion of the founders to ensure that the desired genetic trait will not be lost if anything happens to the founders |
What is the production colony? | Animals actually used for research-bred from the expansion colony |
What is hybrid breeding? | A selective system in which the parents are of different inbred strains-used to transfer a desired mutation from one to the other strain. Offspring are a mixture of the parents |
What are recombinant inbred strains? | Hybrid breeding followed by brother/sister matings or inbreeding of the F1 and subsequent offspring |
What is a coisogenic animal? | An inbred animal with a single mutation-that makes the animal different from the other animals. Ideal for studying effects of one gene |
What are congenic strains? | They are constructed by selectively mating an animal carrying the mutation of interest to an inbred animal from a strain of choice-to fix the mutant gene in the genetic background of subsequent generations |
What is artificial insemination? | Manually placing semen into the reproductive tract of a female in heat |
What is in vitro fertilization? | Involves the union of eggs and sperm outside the body, and then implementing the resulting zygotes |
What are the four stages of the estrous cycle? | Proestrus-eggs develop in ovary, Estrus-ovulation, Metestrus, Diestrus |
What is anestrus? | The long period between breeding seasons in some animals |
What is parturition? | Birth of the young at the natural end of gestation |
What is a foster mother? | A lactating female that rears the young in place of the biological mother. |
What conditions must be met in fostering situations? | -Total litter size must remain about the same to ensure mother has enough milk -foster pups should be close in age to the mother's own pups |
What can variations in the macroenvironment do to a breeding colony? | Failure to copulate, Disruption of the estrous cycle ,abandonment of young, cannibalism |
When should pups be weaned? | When they are capable of reaching food and water |
How can the sexes be differentiated at time of weaning? | Anogenital distance-distance between the anus and the genital papilla-greater in males than in females |
What is a recommended time frame for replacing breeders? | After about 9 months, or after female have had 6-7 litters |
What is animal biosecurity? | All measures to control known or unknown infections in laboratory animals-methods should be in place to minimize risk of infecting established colonies and to deal with disease within the colony it is arises |
What is rederivation? | Process used to clear a colony of infectious disease or to obtain an animal line from a facility where animals cannot be verified as SPF |
What is cryopreservation? | To preserve a genetic line of some animals-freeze at very low temperatures embryos, sperm cells or ovaries |
What is special about cats? | Polyestrous and will breed all year long, induced ovulators |
What is special about rabbits? | Induced ovulators, no distinct estrous cycle; doe is always taken to the buck's cage-if not doe will attack buck |
What is special about dogs? | Estrus occurs every 7-8 months in any season; spontaneous ovulation |
What does farrowing mean? | In pigs ,means giving birth |
What is special about ferrets? | Induced ovulators |