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Intro, Equipment
Intro, Equipment - VTT 234
Question | Answer |
---|---|
What is the centrifuge used for? | To separate substances of different densities, that are in a solution. |
When solid and liquid components are present in the sample, the liquid portion is called? | Supernatant |
When solid and liquid components are present in the sample, the solid portion is called? | Sediment |
Name two types of centrifuges. | Hematocrit and Clinical |
Name two types of clinical centrifuge heads? | Horizontal (also known as "swing arm") and Angled |
Incorrect loading can cause damage to what? | Instrument and to the operator. |
What is a refractometer used to measure? | The refractive index of a solution. |
What is Refraction? | The bending of light rays as they pass from one medium (like air) into another medium (urine,..) with a different optical density. |
What do you use to calibrate a refractometer to zero? | Distilled water |
Total protein will be a number between? | 1-10 |
Specific gravity is always? | A decimal (1.000 - 1.070) |
Most chemistry analyzers used in veterinary practice utilize what? | Photometry, electrochemistry |
What is designed to measure the amount of light transmitted through a solution? | Spectrophotometer |
Electrochemical methods are usually used for the evaluation of? | Electrolytes |
Chemical analyzers are designed with specific electrodes that are configured to allow interaction with how many ions? | One |
What does OSHA stand for? | Occupational Safety and Health Administration |
What safety equipment does OSHA say needs to be available? | Eyewash stations, fire extinguishers, spill kits, biohazard containers, protective gloves |
What are some benefits of a hematology analyzer? | Reduced labor, more complete, accurate, improvement of data reliability |
Instrumentation methods include: | Impedance analyzer, laser-based analyzer, QBC systems |
What is an Impedance Analyzer? | A passage of current across two electrodes, separated by a glass tube with a small opening or aperture. |
Impedance analyzers are calibrated to? | Count the cells based on their size. |
What does QBC stand for? | Quantitative Buffy Coat system |
How does the Quantitative Buffy Coat system work? | It uses differential centrifugation & estimation of cellular elements by measurements on an expanded buffy coat layer, in a specialized microhematocrit tube. |
What are the common measurements in the metric system? | Length (Meter "m"), Mass (Gram "g"), Volume (Liter "L") |
What is a hematocrit centrifuge for? | Designed to only hold microhematocrit tubes. |
What is a clinical centrifuge for? | Accomodates test tubes of many different sizes. There are 2 types, depending on the style of the head. Horizontal and Angled |
What is the angle of specimen tubes in an angled centrifuge? | Usually 52° |
What are laser-based analyzers? | Uses laser beams to determine the size and density of the cells, and evaluates the nuclear and cytoplasmic features of a cell. |
What is the daily maintenance requirement for electronic cell counters? | Flushing the entire system with cleaning solution & fresh diluting solution to keep the aperture open, wiped down, rebooting, intake fan cleaned out. |
Incubators must be capable of sustaining a constant temperature of....? | 37° C |
What is accuracy? | How closely results agree with the true value of the constituent. |
What is precision? | The magnitude of random errors and the reproducibility of measurements. |
What is reliability? | The ability of a method to be both accurate and precise. |
What does OSHA mandate in laboratory practice safety policies? | Safety equipment and supplies must be available. |