Topic 2: Special Microscopes
Quiz yourself by thinking what should be in
each of the black spaces below before clicking
on it to display the answer.
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show | 2 light microscopes combined
Low magnification/Large focus
More powerful than objective lens
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show | Colposcope (Vaginal examination)
Microsurgery
Dissection (papillae)
Parasitology and Pathology
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show | Cartoid filter: Blocks central light
Object scatters light
Object illuminated on a dark field
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Uses for Dark Field Microscope | show 🗑
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Principle of Phase Contrast Microscope | show 🗑
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Theory of Phase Contrast Microscope | show 🗑
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Uses of Phase Contrast Microscope | show 🗑
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Nomarsky Microscope | show 🗑
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show | Prism 2 reassembles the light
Contrast due to:
Difference in refractive index not thickness
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Uses of Nomarsky Microscope | show 🗑
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show | 2 filters
Polarizator - Condensing lens
Analizator - Objective lens
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show | Filters light onto one plane
Analizator is // : Field of vision is bright
Analizator is perp.: Field of vision is dark
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show | Structures that are able to rotate light to another plane.
Shows up bright on a dark field
eg. Crystals
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Use of Polarisation Microscope | show 🗑
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Flouresence Microscope | show 🗑
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Colour of self fluorescing tissues: Collagen Porifines Carotinids Lipofuscin | show 🗑
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show | Flourescine
Acridine Orange
Rhodamine
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show | Laser beam excites molecules
Laser moves across the object
Light focused (w/pin hole)
Records image
Thicker objects
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Light beams in Flouresence Microscopy | show 🗑
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