Topic 2: Special Microscopes
Quiz yourself by thinking what should be in
each of the black spaces below before clicking
on it to display the answer.
Help!
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show | 2 light microscopes combined
Low magnification/Large focus
More powerful than objective lens
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show | Colposcope (Vaginal examination)
Microsurgery
Dissection (papillae)
Parasitology and Pathology
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show | Cartoid filter: Blocks central light
Object scatters light
Object illuminated on a dark field
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show | Unstained organims
Tracking movement of micro-organisms
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show | Varying thicknesses in object
Varying refractive indices
Microscope amplifies these variances
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Theory of Phase Contrast Microscope | show 🗑
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Uses of Phase Contrast Microscope | show 🗑
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show | 2 Prisms
Polarised light
Prism 1: Splits light
Split Light enters object close to eachother
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show | Prism 2 reassembles the light
Contrast due to:
Difference in refractive index not thickness
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Uses of Nomarsky Microscope | show 🗑
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Polarisation Microscope | show 🗑
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Polarizator & Analizator | show 🗑
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show | Structures that are able to rotate light to another plane.
Shows up bright on a dark field
eg. Crystals
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show | Membrane analysis
Diagnositics
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Flouresence Microscope | show 🗑
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show |
Light Blue (Collagen)
Red (Porfirines)
Yellow (Carotinids/Lipofuscin)
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show | Flourescine
Acridine Orange
Rhodamine
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Confocal Laser Scanning Microscope | show 🗑
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Light beams in Flouresence Microscopy | show 🗑
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Created by:
10fang711
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