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Stack #169681

H & E stain

QuestionAnswer
Stainning to desired intensity and stopping the stain Progressive Stain
Tissue is overstained,decolorized or differentiated until desired components contrast w/the background Regressive Stain
Not enough hematoxylin,not stained long enoug,stain overoxidized (old),differentiated too long, bones overdecalcified Pale nuclei
Hematoxilyn too dark, stained too long, sections too thick, differentiation too short Nuclei overstained
Hematoxylin broken down, slides not blued long enough Red/brown Nuclei
PH of eosin is over 5.0, section too thin, dehydrated too long Pale staining w/eosin
sections stained too long, dehydrated too quickly, eosin concentrated, sections too thick Cytoplasm overstained
Metal sheen - unfiltered hematoxylin Blue/Black percipitate on slide
Dont let slides dry out, keep solutions covered, keep absolute alcohol covered, filter hematoxylin, rinse sections well after ammonia waterso the ph of eason will not become basic, dont dehydrate slides too quickly or the eosin will not be differentialted H & E helpful hints
fixation, infiltration w/paraffin, staining 3 steps of tissue preparation
terminate cell metabolism, prevent autolysis, kill viruses, bacteria fungi 4 purposes of fixation
water soluble hematoxilyn
alcohol soluble eosin
nuclei show coalescense of chromatin nuclear bubbling
hematoxylin, absolute ethyl alc, ammonium aluminum sulfate, Distilled water, Mercuric oxide Harris hematoxylin
Aluminum Harris hematoxylin (mordant)
mercuric oxide Harris hematoxylin chemical ripening agent
sodium iodate m/b used instead of mercuric oxide. (sodium iodate is recommended) Harris hematoxylin
(solution A)Ammonium aluminum sulfate, di water, (solution B) hematoxylin, alcohol 95% Delafied hematoxylin
solution A - saturated ammonium aluminum sulfate, Solution B - hematoxylin, age 3-6 mths and test for readiness Delafied hematoxylin
Aluminum Delafied hematoxylin mordant
oxidation occurs naturally with exposure to light and air. Delafied hematoxylin
alcohol functions to preserve Delafied hematoxylin
glycerol stabilizes solution agains overoxidation and aids in preventing rapid evaporation Delafied hematoxylin
hematoxylin, di water, sodium iodate, ammonium or potassium aluminum sulfate, citric acid and chloral hydrate Mayer hematoxylin
aluminum Mayer hematoxylin mordant
sodium iodate Mayer hematoxylin oxidizer
citric acid is added to adjust the pH Mayer hematoxylin
citric acid and chloral hydrate help to prevent scum and precipitates that form Mayer hematoxylin
hematoxylin, alc 95%, di water, glycerol, ammonium or potassium aluminum sulfate, glacial acetic acid Ehrlich hematoxylin
aluminum Ehrlich hematoxylin mordant
ripening c/b accomplished naturally or chemically Ehrlich hematoxylin
this hematoxylin gives a very sharp nuclear stain and m/b used progressively, more commonly used regressively Ehrlich hematoxylin
di water, ethylene glycol, hematoxylin anhydrous, sodium iodate, aluminum sulfate, glacial acetic acid Gill hematoxylin
Ethylene glycol is excellent solvent for hematoxylin Gill hematoxylin
ethylene glycol prevents formation iof surface precipitates Gill hematoxylin
sodium iodate is oxidizer and aluminum sulfate is mordant Gill hematoxylin
crystillanin hematoxylin m/b used instead of anhydrous hem. Gill hematoxylin
Goblet cells will stain with Gill hematoxylin
Solution A - Ferrich chlorided, di water, hydrochloric acid, solution B -hematoxylin, alcohol 95%. Weigert hematoxylin
Celestine blue m/b substituted for hematoxylin in H&E procedure Weigert hematoxlyin
Dye mordant lake is formed Weigert hematoxylin
iron is the mordant Weigert hematoxylin
used progressively 5 to 30 minutes Weigert hematoxlin
negatively charged - anionic,dyes that combine w/cationic positive charged tissue groups plasma stains
most widely used nuclear stain hematoxylin
most widely used plasma stain eosin
mordant/dye combination lake
basic amino acids arginine, histidine, lysine
arginine, histidine, lysine combine w/eosin. (plasma stain)
Created by: nperez