Help
Options
Didn't know it?
click below
click below
Knew it?
click below
click below
Don't Know
Remaining cards (0)
Know
retry
shuffle
restart
0:00
Embed Code - If you would like this activity on your web page, copy the script below and paste it into your web page.
Normal Size Small Size show me how
Normal Size Small Size show me how
Histology ASCP
ASCP Histology Tech: Processing/Instrumentation
| Question | Answer |
|---|---|
| dehydrating agents | alcohol, acetone, dioxane, tetrahydrofuran (last two are universal solvents) |
| incomplete dehydration results in | mushy soft sections |
| the dye-check solution of eosin can only be added to ________, not isopropanol | ethanol (dehydratant) |
| __________ cannot be used in processors or with isopropanol | Drierite (dehydratant) |
| clearing agents must be miscible with ______ and _______. | dehydratant and paraffin |
| a reagent that CANNOT be used for dehydrating tissues is: | benzene (clearnat) |
| paraffin used for embedding is ___ degrees celsius. | 53 degrees celsius |
| one disadvantage using dioxane for processing is | it is TOXIC |
| one xylene disadvantage is | it is likely to harden tissue |
| routine use paraffin melting point should be | 55 to 58 degrees C |
| which is not a clearing agent? chloroform/ethanol/dioxane/xylene | ethanol (dehydratant) |
| overheating of paraffin used for embedding may cause | cracking of the block |
| one advantage of paraffin use is | serial sections are easy to obtain |
| when cutting a block, if the tissue seems hard and brittle... | overheated paraffin |
| SOFT paraffin is most useful when _______ sections are desired. | thick |
| when used for clearing, cedar wood oil must be followed by | xylene (clearant) |
| paraffin is cooled rapidly as possible after embedding to prevent | formation of large crystals |
| dehydration and clearing steps can be omitted when | using water-soluble wax (embedding medium) |
| ETHYL ALCOHOL | BEST DEHYDRATANT!!! PEL=1,000 / 60-65-95-95-absolute |
| denatured alcohol | ethanol + methanol or ethanol + isopropanol (dehydratant) |
| METHYL ALCOHOL | POISONOUS dehydratant, NOT USED ALONE PEL=200 / not for skin; breaks down to formaldehyde; causes blindness and death |
| Dioxane | a UNIVERSAL SOLVENT which is faster, less shrinkage than ethanol, toxic, rarely used / PEL-OSHA=100 /PEL-NIOSH=1 |
| ISOPROPANOL | a dehydratant that CANNOT BE USED IN PLACE OF ETHANOL WHEN STAINING! PEL=400 |
| Acetone | (dehydratant) PEL-OSHA=1,000 / PEL-NIOSH=250 / (rapid, cheap, but absorbs water from the air) USED IN TOUCH PREPS OR FAST FIXES. CAN ALSO BE USED LIKE A UNIVERSAL SOLVENT. |
| BUTYL ALCOHOL | 4 PLANTS AND ANIMAL MATERIAL! PEL=100 (dehydratant) |
| Tertiary Butanol | (dehydratant)odorous, expensive, solidifies at room temperature,MIXES WITH WATER, ETHANOL, XYLENE / PEL=100 |
| Tetrahydrofuran | a universal solvent that is miscible in lower alcohols, water, xylene, toluene, benzene, chloroform, acetone,melted parrafin. less toxic than dioxane. PEL=200 / offensive odor, causes conjunctivitis/ BEST UNIVERSAL SOLVENT |
| PEL=100 / used as clearing agent in most labs | xylene (clearant) |
| Toluene | BEST HYDROCARBON CLEARING AGENT, PEL=50, STEL=150 /not as hard as xylene |
| Benzene | fast, does not over-harden, WILL HARDEN MUSCLE, TENDON, UTERUS more than TOLUENE // PEL=10 |
| XYLENE SUBSTITUTES ARE | ALIPHATIC HYDROCARBONS AND LIMONENE |
| CHLOROFORM PEL=50 | chloroform is carcinogenic |
| water soluble waxes | CARBOWAX: dehydrating and clearing unnecessary. Infiltrates tissue directly from fixative.BAD: floating out occurs |
| a nitrocellular compound used for embedding (i. Parloidion). storage blocks held in 80% alcohol. hardened with chloroform. mixed with ether and absolute alcohol to prepare. | celloidin |
| great for undecalcified bone, kidney, bone marrow, lymph node biopsies | GMA Glycol Methacrylate , acrylic resin which is miscible with water. |
| 5% aqueous formic acid will | decalcify most bones in 3 to 5 days |
| celloidin MUST BE | dehydrated |
| ethylene glycol monoethyl ether | a dehydratant that retains tissues for extended periods of time. more than ethanol or 70% alcohol |
| sections processed by _______ method show less shrinkage than when processed by paraffin. | celloidin |
| best processing for enzyme demonstration | frozen sections |
| ion exchange resin & electrolytic methods of decalcification are considered | acid methods |
| these agents EDTA)bind certain metals | chelating agents |
| 30% sucrose | best way to get frozen sections from formalin fixed, unprocessed tissue, by infiltrating in this |
Created by:
alexinem
Popular Histology sets