Histology Microscopy Word Scramble
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| Term | Definition |
| Stereo Microscope | 2 light microscopes combined Low magnification/Large focus More powerful than objective lens |
| Uses for stereo microscope | Colposcope (Vaginal examination) Microsurgery Dissection (papillae) Parasitology and Pathology |
| Dark Field Microscope | Cartoid filter: Blocks central light Object scatters light Object illuminated on a dark field |
| Uses for Dark Field Microscope | Unstained organims Tracking movement of micro-organisms |
| Principle of Phase Contrast Microscope | Varying thicknesses in object Varying refractive indices Microscope amplifies these variances |
| Theory of Phase Contrast Microscope | Annulus diaphragm - Over condenser Ring of light allowed through Phase Ring - In objective lens Changes wavelength by 0.25 (lambda) Combines refracted and diffracted light |
| Uses of Phase Contrast Microscope | Living cells Unstained cells Cell cultures |
| Nomarsky Microscope | 2 Prisms Polarised light Prism 1: Splits light Split Light enters object close to eachother |
| Nomarsky Microscope (Prism 2) | Prism 2 reassembles the light Contrast due to: Difference in refractive index not thickness |
| Uses of Nomarsky Microscope | Cell cultures Unstained cells Living cells Immunostained material |
| Polarisation Microscope | 2 filters Polarizator - Condensing lens Analizator - Objective lens |
| Polarizator & Analizator | Filters light onto one plane Analizator is // : Field of vision is bright Analizator is perp.: Field of vision is dark |
| Anisotropic | Structures that are able to rotate light to another plane. Shows up bright on a dark field eg. Crystals |
| Use of Polarisation Microscope | Membrane analysis Diagnositics |
| Flouresence Microscope | UV light source 2 filters pre and post specimen Specimen absorbs light, emits a light with bigger wavelength |
| Colour of self fluorescing tissues: Collagen Porifines Carotinids Lipofuscin | Light Blue (Collagen) Red (Porfirines) Yellow (Carotinids/Lipofuscin) |
| Flourescent Dyes (F.A.R.) | Flourescine Acridine Orange Rhodamine |
| Confocal Laser Scanning Microscope | Laser beam excites molecules Laser moves across the object Light focused (w/pin hole) Records image Thicker objects |
| Light beams in Flouresence Microscopy | Barrier 1: 450-490 nm Barrier 2: <510 nm (reflected) > 510 nm (pass) Barrier 3: 520 to 560 nm (pass through) |
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