agglutination tests, Nephelometry, Flow Cytometry, Molecular Techniques,
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| What is Prozone? | area of antibody excess
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| what is postzone? | area of antigen excess
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| what is agglutination? | visible aggregates of particulate matter caused by antigen-antibody reactions
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| what is direct agglutination? | antigens occurring naturally on microbes
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| list four uses of the direct Coombs test (DAT) | autoimmune hemolytic anemia, HDN, transfusion reactions, RBC drug sensitization.
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| List two main uses of the indirect Coombs test (IAT) | detects unexpected antibody in the patient's serum and it is used in the antibody screening part of compatibility testing for blood transfusions.
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| Name the instrument used to measure antigen-antibody complexes in a solution. | Nephelometer
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| State the principles of operation of a nephelometer | when light passes through a suspension, light is scattered and detected by a photodlode
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| list 3 types of patient serum specimens that cannot be used for nephelometry testing. | Hemolyzed, lipemic, turbid (bacterially contaminated)
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| list the main technologies used in flow cytometry | optics, fluids, lasers, fluorochrome conjugated monoclonal antibodies and computers.
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| list 5 common kinds of patient specimens analyzed by flow cytometry | lymph nodes, peripheral blood, bone marrow, tumor, other tissues.
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