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MLT Immunology

agglutination tests, Nephelometry, Flow Cytometry, Molecular Techniques,

QuestionAnswer
What is Prozone? area of antibody excess
what is postzone? area of antigen excess
what is agglutination? visible aggregates of particulate matter caused by antigen-antibody reactions
what is direct agglutination? antigens occurring naturally on microbes
list four uses of the direct Coombs test (DAT) autoimmune hemolytic anemia, HDN, transfusion reactions, RBC drug sensitization.
List two main uses of the indirect Coombs test (IAT) detects unexpected antibody in the patient's serum and it is used in the antibody screening part of compatibility testing for blood transfusions.
Name the instrument used to measure antigen-antibody complexes in a solution. Nephelometer
State the principles of operation of a nephelometer when light passes through a suspension, light is scattered and detected by a photodlode
list 3 types of patient serum specimens that cannot be used for nephelometry testing. Hemolyzed, lipemic, turbid (bacterially contaminated)
list the main technologies used in flow cytometry optics, fluids, lasers, fluorochrome conjugated monoclonal antibodies and computers.
list 5 common kinds of patient specimens analyzed by flow cytometry lymph nodes, peripheral blood, bone marrow, tumor, other tissues.
Created by: delacuesta21