Ch 40, 41, 43
Quiz yourself by thinking what should be in
each of the black spaces below before clicking
on it to display the answer.
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| What can you culture? | Urin, Throat, Blood, Stool, Wound, Sputum, Nose, CSF
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| What are the 7 rights of Spec Collection | right site, right amount, right container, right medium, right lable, right amount of time, right temp.
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| How big do the bacteria colonies have to be to be bad? | greater than 100,000
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| name the different types of bacteria? | cocci(round in shape), bacilli(rod-shaped and can have rounded), spirilla(spiral-shaped)
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| How hot does an auto clave have to get? | 121%C
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| What is the proper name to which our time is referred to? | Greenwich time
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| What is the basic unit of measurement for weight in the metric system? | Milligram
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| What test measures the oxygen carrying capacity of the blood? | Hemoglobin
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| What are red blood cells called? | erythrocytes
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| what are white blood cells called? | leukocytes
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| what are platelets called? | thrombocytes
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| What is Hematopoiesis? | formation of blood cells
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| What does a CBC test generally include? | Hemoglobin determination, Hematocrit determination, RBC count, WBC count, Erythrocyte indices, differential WBC count
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| What are the parts of Hemoglobin molecule? | Globin(4), heme(4) and iron(1)
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| What are the two basic groups of leukocytes? | granulocytes(which contain granules) and agranulocytes(which do not contain granules)
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| How are leukocytes identified? | by the contents of the cytoplasm
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| Name the three granulocytes? | neutrophils, basophils, and eosinophils
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| What are the names of the sizes of erythrocytes? | normocytic(normal size), macrocytic(larger), and microcytic(smaller)
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| When the erythrocytes show marked variation in size this condition is called? | anisocytosis
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| What is the name for the proper amount of hemoglobin? | normochromic
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| those that do not have enough hemoglobin are called? | hypochromic
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| What is the normal amout of platelets? | 140,000 to 400,000/cm ul
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| How many steps does coagulation have? | 13
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| What does a capillary tube with a blue line contain? | antiecoagulant
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| What are the layers in which a centrifuged tube separates? | clear liquid=serum or plasma, cloudy white layer=buffy coat, and at bottom blood colored=packed RBC's
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| What is the daily iron requirments for adult females? | 2.0mg
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| What does POL mean? | physicians' office laboratory
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| What is a pathogen? | causative microorganism
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| what is a holding media? | something that keeps a specimen on a swab moist until it is cultured?
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| What is Taxonomy? | classification of living organisms
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| What is the CLIA? | Clinical Laboratory Improvement Act
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| What does parasitology? | identification of parasites
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| What does virology mean? | identification of viruses
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| what does mycology do? | identification of fungi
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| What are infections acquired in hospitals referred to? | nosocomial
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| Microorganisms have two distinct groups one what are they? | aerobic(with oxygen) and anaerobic(no oxygen)
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| What is a wet-mount? | nothing is added used to observe motility.
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| What is another solution that can be used in a wet-mount? | KOH(potassium hydroxide)
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| What are the different classifications of media? | Basic, Differential, Selective, and Enriched
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| What is a basic media? | used for general purposes and do not contain added nutrients. They will support the growth of many Gram-negative and Gram-positive organisms.
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| What is a differential media? | Contains substances that alter the appearance of some types of organisms and not other types.
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| What is a selective media? | supports the growth of one type of organism, whereas inhibiting the growth of another.
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| What is a enriched media? | contains substances that inhibit certain bacteria from growing. These media work well with cultures from sites that possess normal flora, such as the throat.
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| What is Silica and where is it found? | it is glass and it is found in a red top serum sperator tube.
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| How long does a serum seperator tube sit? | 30min to an hour
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| What is culturing mean? | to isolate a disease-causing microorganism for identification.
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| What is the medium for culturing? | agar a gelatin-like substance mixed with nutrients
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