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Ch 40, 41, 43
Question | Answer |
---|---|
What can you culture? | Urin, Throat, Blood, Stool, Wound, Sputum, Nose, CSF |
What are the 7 rights of Spec Collection | right site, right amount, right container, right medium, right lable, right amount of time, right temp. |
How big do the bacteria colonies have to be to be bad? | greater than 100,000 |
name the different types of bacteria? | cocci(round in shape), bacilli(rod-shaped and can have rounded), spirilla(spiral-shaped) |
How hot does an auto clave have to get? | 121%C |
What is the proper name to which our time is referred to? | Greenwich time |
What is the basic unit of measurement for weight in the metric system? | Milligram |
What test measures the oxygen carrying capacity of the blood? | Hemoglobin |
What are red blood cells called? | erythrocytes |
what are white blood cells called? | leukocytes |
what are platelets called? | thrombocytes |
What is Hematopoiesis? | formation of blood cells |
What does a CBC test generally include? | Hemoglobin determination, Hematocrit determination, RBC count, WBC count, Erythrocyte indices, differential WBC count |
What are the parts of Hemoglobin molecule? | Globin(4), heme(4) and iron(1) |
What are the two basic groups of leukocytes? | granulocytes(which contain granules) and agranulocytes(which do not contain granules) |
How are leukocytes identified? | by the contents of the cytoplasm |
Name the three granulocytes? | neutrophils, basophils, and eosinophils |
What are the names of the sizes of erythrocytes? | normocytic(normal size), macrocytic(larger), and microcytic(smaller) |
When the erythrocytes show marked variation in size this condition is called? | anisocytosis |
What is the name for the proper amount of hemoglobin? | normochromic |
those that do not have enough hemoglobin are called? | hypochromic |
What is the normal amout of platelets? | 140,000 to 400,000/cm ul |
How many steps does coagulation have? | 13 |
What does a capillary tube with a blue line contain? | antiecoagulant |
What are the layers in which a centrifuged tube separates? | clear liquid=serum or plasma, cloudy white layer=buffy coat, and at bottom blood colored=packed RBC's |
What is the daily iron requirments for adult females? | 2.0mg |
What does POL mean? | physicians' office laboratory |
What is a pathogen? | causative microorganism |
what is a holding media? | something that keeps a specimen on a swab moist until it is cultured? |
What is Taxonomy? | classification of living organisms |
What is the CLIA? | Clinical Laboratory Improvement Act |
What does parasitology? | identification of parasites |
What does virology mean? | identification of viruses |
what does mycology do? | identification of fungi |
What are infections acquired in hospitals referred to? | nosocomial |
Microorganisms have two distinct groups one what are they? | aerobic(with oxygen) and anaerobic(no oxygen) |
What is a wet-mount? | nothing is added used to observe motility. |
What is another solution that can be used in a wet-mount? | KOH(potassium hydroxide) |
What are the different classifications of media? | Basic, Differential, Selective, and Enriched |
What is a basic media? | used for general purposes and do not contain added nutrients. They will support the growth of many Gram-negative and Gram-positive organisms. |
What is a differential media? | Contains substances that alter the appearance of some types of organisms and not other types. |
What is a selective media? | supports the growth of one type of organism, whereas inhibiting the growth of another. |
What is a enriched media? | contains substances that inhibit certain bacteria from growing. These media work well with cultures from sites that possess normal flora, such as the throat. |
What is Silica and where is it found? | it is glass and it is found in a red top serum sperator tube. |
How long does a serum seperator tube sit? | 30min to an hour |
What is culturing mean? | to isolate a disease-causing microorganism for identification. |
What is the medium for culturing? | agar a gelatin-like substance mixed with nutrients |