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Microbiology Specimen Collection, Transport and Processing

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Body Fluids (peritoneal, synovial, pleural, etc)   Sterile, screw-cap container or anaerobic transporter   Disinfect aspiration site with alcohol swab and Betadine   show Transport immediately to laboratory  
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Eye   Aerobic transport swab   show   Trahsport swabay be stored up to 24 hrs at room temp  
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Stool   Clean, leak proof container   Outpatients: At minimum, three specimens are collected every other day   Transport to lab within 24 hr at 4*C   show
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Rectal swab   Swab placed directly in enteric transport medium   show Insert swab approx 1 in past anal sphincter   Store at 4*C, transport within 24 hr to lab and place within 72 hr  
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Gonorrhea culture   show Wipe away exudate before obtaining culutre specimen, obtain culture specimen with swab   Do not refrigerate   Transport to lab within 2 hrs  
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Chlamydia culture   Specialized transport medium containing antibiotics   show Transport immediately on ice to lab   Store up to 24 hr at 4*C; inoculate within 15 min of collection if swab is not on ice  
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Skin scraping for fungal culture   Clean, screw-top tube   Wipe skin with alcohol prep pad   show Can be held indefinitely at room temp but best to process within 72 hrs of collection  
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Sputum   Sterile, screw-cap container   show Have patient collect from deep cough; do not collect saliva   Store at 4*C and plate within 24 hr  
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show Transport swab   Have patient sitting with head tilted back   Swab pharynx and tonsils,not mouth, tongue or teeth   Transport and plate within 24 hr; room temp storage  
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Ova and parasite (O&P)   OP&P transport device (with formalin and PVA)   show Wait 7-10 days if pt has been taking Pepto-Bismol, Kaopectate, or MOM   Store at room temp and deliver to lab within 24 hr  
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Urine   Sterile, screw-cap container   show Hold at 4"C and deliver to lab within 24 hr   Hold at 4*C (39.2*F) and plate within 24 hr  
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show Aerobic transport swab   Wipe area with sterile saline or alcohol prep pad before collection   Rotate swab while gently swiping wound   Transport and plate within 24 Hr; room temperature storage  
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Deep wound or abscess   Anaerobic transport device   show Aspirate material, excise tissue, or insert swab deep into wound   Store at room temperature; transport to labe within 4 hr  
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Blood   show Disinfect venipuncture site with alcohol swab and Betadine   Draw blood during febrile episodes (usually drawn- two sets from opposite arms)   Deliver to lab within 2 hrs; incubate at 37*C (98.6*F)  
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Created by: cskinner
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