Term | Definition |
Mycobacteria | slender, nonmotile, slow-growing, acid-fast, obigate aerobes, resist gram staining |
Ziehl-Neelsen | acid fast stain, methylene blue counterstain, uses heat |
Kinyoun stain | acid fast stain, methylene blue counterstain, cold stain |
Auramine-rhodamine | acid fast stain, used to visualize acid fast bacilli using fluroescence microscopy, not as specific |
Acridine orange | acid fast stain, mthylene blue counterstain, fluorescent |
Gram stain resistant (Mycobacteria) | High lipid content in cell walls |
Runyon classification | based on rate of growth, production of yellow pigment and whether pigment was produced in dark or after light exposure |
M. Tuberculosis | common in Mexico, buff colonies, niacin positive, PPD skin test |
M. Leprae | Hansen's disease, leprosy |
Decontaminate and digestion | to liquefy sample to allow chemical decontaminating agent to contact and kill non-mycobacterial organisms |
Agar for mycobacteria | Lowenstein-Jensen, Petragnami, American Thoracic Society Agar, Middlebrook agar |