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Microbiology Items
Microbiology
| Question | Answer |
|---|---|
| Transport Media | Stuart and Amies |
| Gram positive appearance (color) | Purple |
| Gram negative appearance (color) | Pink or red |
| Cocci (pleural - coccus) | round bacteria |
| Bacilli (plueral - bacillus) | rod shaped bacteria |
| strepto | bacteria forming a chain (think a strep/strip thatlooks line a line) |
| staphylo | bacteria forming a cluster (think of a staff (staph) of workers that are a group that work together) |
| The percentage of all microorganisms that cause disease is_____ | Fewer than 1% of microorganisms are pathogens |
| Pathogen | A disease causing microorganism |
| Some microorganisms are normally found in the body | True - we have a 'normal flora' of bacteria that help with functions of the body such as digestion. |
| POL stands for______________/ | Physician's Office Laboratory |
| What bacterial culture cannot be refrigerated after collection? | Gonorrhea culture (agar plate should be body temperature prior to inoculation as well) |
| Fungal infections may be determined by________________ | Wet mount (potassium hydroxide - KOH and/or saline solution)smear and slide for microscopic exam and fungal culture |
| Spirillum (pleural spirilla) | Spiral shaped bacteria |
| Tightly coiled spirilla | Spirochetes |
| diplo- | term used to indicated double or two eg. bacteria found in pairs such as diplococcus |
| tetrads | groups of four |
| sarcinae | packets of eight or sixteen |
| anaerobes | bacteria that die in the presence of oxygen |
| aerobes | bacteria that require oxygen to live |
| facultative anaerobes | bacteria that are flexible concerning oxygen requirements - can survive with or without oxygen |
| prokaryotes | a unicellular organism with cells that lack a membrane-bound nucleus eg. bacteria |
| eukaryote | single celled or multicellular organisms with cells that contain a distince membrane-bound nucleus eg. fungus |
| helminths | worms |
| Protozoa | single-celled parasitic eukaryotes |
| all-purpose or nutritive culture media | supports growth of a wide variety of bacteria |
| selective culture media | supports growth of one type of organism while inhibiting the grow of others. |
| differential culture media | culture media that contains chemicals or dyes that alter the appearance of certain types of bacteria |
| enriched culture media | contains comles organic materials that certain fastidious species must have to multiply eg. blood agar for growth of streptococcus pyogenes |
| gram stain chemicals used | primary dye (crystal violet), mordant (grams iodine), decolorizer, counterstain (safranin) |
| Acid-fast stain | Used to identify Mycobacterium species (pathogen of TB and MAC a soil organism causing respiratory illness). The process disrupts the waxy cell wall to facilitate staining. AFB = acid fast bacilli |
| Ziehl-Neelsen and Kinyoun protocol | Processes of acid-fast staining |
| CLIA | Clinical Laboratory Improvement Amendment passed in 1988 to oversee proper lab procedures |
Created by:
cskinner
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