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Phlebotomy study
Order of blood draw and color-coded tops for blood draw
| Question | Answer |
|---|---|
| Order of Blood Draw (First) Blood Culture Tubes: | Yellow-Sterile are first. These tubes are sterile. The phlebotomist does not want to transfer from nonsterile tube to a sterile tube. |
| Order of Blood Draw (Second) Coagulation Tests: | Light Blue Tops are second, (next). These tubes are always drawn before tubes with other kinds of anticoagulants. |
| Blue Tubes | Phlebotomist must be aware NOT to have cross contamination with other additives that could interfere with coagulation. |
| Order of Blood Draw (Third) Clot Activators: | Red and or Red/Gray (Gold) and plastic red top tubes are next. These tubes contain clot activators. AGAIN ALL EXCEPT THE RED GLASS TUBE. NO ADDS. |
| Red Glass Top Tubes: | It is safe to draw glass red top tubes because these tubes do not contain additives. |
| Order of Blood Draw (Fourth) Heparin: (Green Top Tubes) | Additive-anticoagulant sodium heparin or lithium heparin. |
| Order of Blood Draw (Fifth) (Lavender) | Lavender Top tubes The EDTA binds to calcium and may give a falsely low calcium and falsely high potassium reading. Lavendar tubes are drawn near the end of the multi-tube draw. |
| Order of Blood Draw (Last) Contains Potassium Oxalate: (Gray) | The Gray Top Tube: This tube can elevate the potassium levels measured in an Electrolyte Analysis. It can damage cel membranes |
| Sodium Fluoride: | additive to Gray Top tubes. |
| Red Glass Tube: | Test include chemistry, serology, blood bank. But mostly Theraputic drugs. |
| Red Glass Tube Additives: | None, (Specimen): Serum, separated by centrifuge after clotting. |
| Red Plastic Tube: | Tests include chemistry, serology |
| LIght Blue Tube: | Tests include Coagulation Testing. |
| Light Blue Tube Additives: | Sodium Citrate, (Specimen) Plasma, Inversion (3-4 times). Notes on Process: Must fill tube completely to maintain the ratio of nine parts blood to one part sodium citrate. |
| Lavender Tube | Tests include Complete blood count (CBC), WBC, platelet count. |
| Lavender Tube Additive: | EDTA (Specimen)Whole Blood, Inversion (8). Notes on Process: None; most common blood test ordered, so lavender tops are the most frequently used. |
| Gold or Red/Gray Stopper Tube: (SST)Serum Separator Tube) | Tests include Most blood Chemistry Panels, Sodium, Potassium, electrolytes. |
| Gold or Red/Gray Tube Additive: (SST's) | Clot activators, thixotropic gel. (Specimen) Serum. Inversion (5). Notes on Process: None; a.k.a., serum separator tubes (SST;s), star tubes, tiger top or jungles top. |
| Gray Tube: | Tests include Lactic acid measurement, glucose tolerance test (GTT), fasting blood sugar (FBS), blood alchol level. |
| Gray Tube Additives: | Iodoacetate, Sodium fluoride; may also have anticoagulant potassium oxalate or heparin. (Specimen) Plasma. Inversion (8. Notes: None |
| Black Tube: | Tests include sedimentation rate. |
| Black Tube Additives: | sodium citrate. (Specimen) Whole blood, Inversion number(0). Notes on process. No inversions because everything must settle in a sedimentation study; must fill tube completely to maintain the ration of four parts blood to one part sodium citrate. |
| Green Tube: (PST) | Tests include STAT blood chemistry, ammomia, electrolytes, arterial blood gases (ABG), human leukocyte antigens (HLA), chromosome analysis. |
| Green Tube Additives: | Heparin, (Specimen), plasma, Inversion (8). Notes: None |
| Light Green or green/gray stopper: | Tests inclue, STAT pottasium. |
| Light Green or green/gray stopper Additives: | Heparin; thixotropic gel, (Specimen), plasma. Inversion (8) Notes on Process: None, a.k.a, plasma separator. |
| Orange or Yellow/Gray stopper: | Tests include STAT blood chemistry. |
| Orange or Yellow/Gray Additives: | Thrombin, (Specimen): Serum, Inversion (8). Notes on Process: allows for five minutes of clotting time for patients on anticoagulant therapy. |
| Royal Blue: | Tests include: toxicology, trace metals, nutritional analysis |
| Royal Blue stopper Additives: | Heparin, EDTA or none, (Specimen) Plasma, or Serum. Inversion number with heparin and EDTA-8, with no additives - 0. No notes. |
| Tan Stopper: | Tests include lead analysis. |
| Tan Additive: | Heparin, (Specimen), Plasma. Inversion (8). No notes |
| Yellow Sterile Tube: | Tests include, Blood cultures. Contains a nutrient broth or referred to as media, which encourages te growth of microorganisms. |
| Yellow Sterile Additives: Blood Cultures | Sodium polyanethol sulfonate (SPS). Inhibits Phagocytosis, Encourages growth of microrganisms. Notes on Process: Recovers microorganisms that are causing blood infections. |
| Yellow (ACD) Acid citrate dextrose. | Tests include: Human leukocyte antigen (HLA) studies; used for paternity testing, tissue typing, DNA and human leukocyte antigen (HLA) |
| Yellow (Non-Sterile) Additives: Do Not confuse with Sterile. | Acid citrate dextrose. (Specimen) Whole Blood, Inversion (8). No notes. |
| Pink Tube: | Tests Include Hematology, Blood Bank. |
| Pink Tube Additives: | EDTA, (Specimen) Whole Blood, Plasma. Inversion (8) mostly used for blood bank Notes on process: Pink tube is similar to the lavender tube, but its closure and label must meet the standard set by the American Association of Blood Bank. |
Created by:
drehmon2237
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