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OrderOfDrawAdditives

Phlebotomy Order of Draw, Tube colors, & Additives

QuestionAnswer
ANTICOAGULANTS (common) EDTA; Citrate (sodium); Heparin (ammonium, lithium, sodium); Oxalates (potassium)
SPECIAL USE ANTICOAGULANTS Acid Citrate Dextrose (ACD); Citrate-Phosphate-Dextrose (CPD); Sodium Polyanethol Sulfonate (SPS)
ANTI-GLYCOLYTIC AGENTS Sodium Fluoride
CLOT ACTIVATORS Silica (glass); Celite (inert clay); Thrombin; SSTs = Serum separator tubes
THIXOTROPIC GEL SEPARATOR density of gel is between that of cells and serum or plasma
TRACE-ELEMENT-FREE TUBES tubes are made from materials that are as free of trace element contamination as possible; they typically contain EDTA, heparin, or no additive
LIGHT BLUE ADDITIVE=Sodium Citrate//DEPT.=Coagulation
RED GLASS ADDITIVE=none//DEPT.=Chemistry, Blood Bank, Serology, Immunology
RED PLASTIC ADDITIVE=clot activator//DEPT.=Chemistry
RED/LIGHT GRAY PLASTIC ADDITIVE=nonadditive//DEPT.=NA(discard tube only)
RED/BLACK(tiger) or GOLD or RED/GOLD ADDITIVE=clot activator & gel separator//DEPT.=Chemistry
GREEN/GRAY or LIGHT GREEN ADDITIVE=Lithium heparin & gel separator//DEPT.=Chemistry
GREEN ADDITIVE=Lithium Heparin or Sodium Heparin//DEPT.=Chemistry
LAVENDER or PINK ADDITIVE=EDTA//DEPT.=Hematology, Blood Bank
GRAY ADDITIVE=Sodium fluoride or Sodium fluoride & potassium oxalate or Sodium fluoride & EDTA//DEPT.=CHEMISTRY
ORANGE or GRAY/YELLOW ADDITIVE=Thrombin//DEPT.=Chemistry
ROYAL BLUE ADDITIVE=none(red label) or EDTA(lavender label) or sodium heparin(green label)//DEPT.=Chemistry
TAN GLASS TUBE ADDITIVE=Sodium heparin//DEPT.=Chemistry
TAN PLASTIC TUBE ADDITIVE=EDTA//DEPT.=Chemistry(lead levels)
YELLOW ADDITIVE=SPS(sodium polyanethol sulfonate) or ACD(acid citrate dextrose)//DEPT.=Microbiology(SPS) or Blood Bank, Immunohematology(ACD)
ORDER OF DRAW (CLSI) 1.Sterile tube (blood culture) 2.Light-blue top (coagulation tube) 3.Serum tube with or w/out clot activator, with or w/o gel 4.Heparin tube with or w/o gel plasma separator 5.EDTA 6.glycolytic inhibitor
ORDER OF DRAW (color tube) 1.Yellow SPS/Sterile media bottles 2.Lt. blue 3.Red glass 4.Red plastic 5.Gold/red-gray 6.Lt. green/green-gray/tan glass/green 7.Lav./pink/pearl/royal blue/tan plastic 11.Gray
PT/PTT prothrombin time/partial thromboplastin time INVERT 5-8 times-> must draw discard tube if this is first tube drawn!!
To get serum, use: Plain Tubes or Clot Activators (CAT)
To get plasma, use: Anticoagulants
BLOOD CULTURES Yellow SPS, sterile media bottles
COAGULATION STUDIES Light Blue,
SERUM STUDIES Gold, Red Glass, Red Plastic
PLASMA STUDIES HEPARIN -> green,light green,tan glass//EDTA->lavender,pink,royal blue,pearl, tan plastic//O&F->Gray
LAVENDER TUBE Used for CBC
Created by: kmaresco
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