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Phlebotomy study
Tube Additives
Question | Answer |
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All tubes contain at least one additive..Not Red Glass Tube. | Additives Include: Anticoagulants, Clot Activators, thixotropic gel to separate components, Preservatives and Inhibitors of various cellular reactions to maintain the integrity of the specimen. |
Additives | Must be inverted and mixed immediately after removal. |
Anticoagulants | Prevent clotting (coagulation) sodium or potassium ethylenediaminetetraacetic acid (EDTA) binds calcium, which will inhibit coagulation of th eblood. |
Binding Calcium, other additives. | Sodium Citrate, Potassium Oxalate, Sodium Ployanethol sulfonate (SPS) |
Heparin inhibits clotting | By preventin the conversion of prothrombin to thrombin. |
The phlebotomist must make sure to fill the tube correctly | So that there is the correct ratio of blood to anticoagulant |
Choice of Anticoagulant | Determines by the test done. |
EDTA | Preserves blood cell integrity, prevents platelet clumping, but interferes with coagulation studies. |
Socium citrate: | Used for coagulation studies. |
SPS | Used for blood cultures because it inhibits certain immune system components that could destroy blood-borne bacteria |
Will also neutralize any antibiotic the patient may be taking | SPS |
Heparin | Preffered for plasma chemistry and blood gas determinations |
Pottasium Oxalate | Used for glucose determination may be combined with sodium fluoride. Inhibits glycolysis. |
Clot activators promote coagulation (clotting) | Thrombin increases clotting and is used for STAT serum chemistry or if the patient is on anticoagulants |
Glass or silica promotes clotting by | Providing more surface area for platelet activation. |
Clot activators | maybe adhered to the side of the tube. |
Invert the tube | AT LEAST 5 TIMES...To make sure it becomes in contact with activator. |
Thixotropic gel: | Synthetic Substance with a density in between that of cells and blood serum or plasma. |
As a result, when patient's blood is drawn and centrifuged: | Thixotropic gel turns to liquid and moves between the lower cell layer and the upper serum or plasma layer. Gel hardens, and forms a barrier allowing easy separation. |