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blood tubes

tube top

ColorAdditiveMode of ActionUses
Red Top None Blood clots, and the serum is seperated by centrifugation Chemistries, Immunology and Serology, Blood Bank (Crossmatch)
Purple Top EDTA Forms calcium salts to remove calcium Hematolgy (CBC) and Blood Bank (crossmatch); requires full-draw -invert 8 times to prevent clotting and platelet clumping
Light Blue Top Sodium Citrate Forms calcium salts to remove calcium Coagulation tests (protime and prothrombin time), full draw required
Green Top Sodium heparin or lithium heparin Inactivates thrombin and thromboplastin For lithium level, use sodium heparin. For ammonia level, use sodium or lithium heparin
Dark Blue Top EDTA Tube is designed to contain no contaminating metals Trace elements testing (zinc, copper, lead, mercury) and toxicology
Black Top Sodium citrate (buffered) Forms calcium salts to remove calcium Westergren Sedimentation Rate; requires full draw
Yellow-Black Broth mixture Preserves viability of microorganisms Microbiology- aerobes, anaerobes, fungi
Pink Top Potassium EDTA forms calcium salts Molecular/viral load testing
Light Green Top Plasma Seperating Tube (PST) with Lithium heparin Anticoagulates with lithium heparin; Plasma is separated with PST gel at the bottom of the tube Chemistries
Orange Top thrombin quickly clots blood STAT serum chemistries
Light Gray Top Sodium flouride and potassium oxalate Antiglycolytic agent preserves glucose up to 5 days Glucoses, requires full draw (may cause hemolysis if short draw)
Yellow ACD (acid-citrate-dextrose) complement inactivation HLA tissue typing, paternity testing, DNA studies
Gold Top None Serum seperator tube (SST) contains a gel at the bottom to seperate blood from serum on centrifugation Chemestries, Immunology and Serology
Light Brown Top Sodium Heparin Inactivates thrombin and thromboplastin; contains virtually no lead Serum lead determination
Created by: andrea646234
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