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Med Lab #1

Tubes- Phlebotomy

ColorAdditiveMode of ActionUses
Green Top Sodium Heparin or Lithium Heparin Inactivates thrombin and thromboplastin For lithium leverls, use sodium heparin For ammonia level, use sodium or lithium heparin
Light Brown Top Sodium Heparin Inactivates thrombin and thromboplastin; conatins virtually no lead Serum lead determination
Red Top None Blood clots, and the serum is separated by centrifugation Chemistries, immunology and serology, blood bank (cross match)
Light Gray Top Sodium flouride and potassium oxalate Antiglycolytic agents preserves glucose up to 5 days Glucoses, requires full draw (may cause hemolysis if short draw)
Dark Blue Top EDTA Tube is designed to contain no contaminating metals Trace element testing (zinc, copper, lead, mercury) and toxicology
Light Green Top Plasma Separating Tube (PST) with Lithium Heparin Anticoagulates with lithium heparin; plasama is separated with PST gel at the bottom of the tube Chemistries
Yellow Top ACD (acid-citrate-dextrose) Complement inactivation HLA tissue typing, paternity testing, DNA studies
Black Top Sodium Citrate (Buffered) Forms calcium salts to remove calcium Westergren Sedimentation Rate; requires full draw
Purple Top EDTA Forms calcium salts to remove calcium Hematology (CBC) and Blood Bank (Crossmatch); requires a full draw-invert 8 times to prevent clotting and platelet clumping
Orange Top Thrombin Quickly clots blood STAT serum chemistries
Gold Top None Serum separator tube (SST) contains a gel at the bottom to separate blood from serun on centrifugation Chemistries, Immunology, Serology
Yellow/Black Top Broth Mixture Preserves viability of microorganisms Microbiolgy- Aerobes, anaerobes, fungi
Light Blue Top Sodium Citrate Forms calcium salts to remove calcium Coagulation tests (protime and prothrombin time), fulldraw required
Pink Top Potassium EDTA Forms calcium salts molecular/viral load testing
Created by: Livia Alvarez
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