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AMCA Phlebotomy Exam
Test Tubes, Additives and Tests
| Question | Answer |
|---|---|
| What are YELLOW tubes used for? | Blood culture and sterile specimens |
| What are BLUE tubes used for? | Coagulation tests (PT, PTT, fibrinogen) |
| What are RED tubes used for? | Serum determinations in chemistry testing and blood bank testing. |
| What are GREEN tubes used for? | Routine chemistry testing |
| What are LAVENDER tubes used for? | Hematology testing (CBC, reticulocyte count, erythrocyte sedimentation). |
| What are GRAY tubes used for? | Glucose tolerance and lactic acid measurement. |
| What are ROYAL BLUE and TAN tubes used for? | Used to collect samples for nutritional studies, therapeutic drug monitoring and toxicology. Also used for testing aluminum, copper, arsenic, calcium, etc. Tan topped is used for lead testing. |
| What is the additive in Yellow stopper tubes? | Sodium Polyanetholesulfonate |
| What is the additive in Blue stopper tubes? | Sodium Citrate |
| Which additive prevents coagulation by binding calcium? | Sodium Citrate |
| Which color tube stopper does not require an additive? | Red |
| What additive *can* (not required) be added to RED stopper tubes? | Thixotropic gel |
| What is the additive in GREEN stopper tubes? | Sodium heparin and lithium heparin - natural anticoagulants that inhibits thrombin. |
| What is the additive in LAVENDER stopper tubes? | Ethylenediaminetetraacetic Acid (EDTA) |
| What does the additive Ethylenediaminetetraacetic Acid (EDTA) do? | Binds the calcium needed for clot formation. |
| What is the additive in GRAY stopper tubes? | Sodium fluoride and Potassium Oxalate |
| What is the additive in TAN stopper tubes? | EDTA - Ethylenediaminetetraacetic Acid |
| What is the correct order of draw when performing a venipuncture? | 1. Blood Cultures 2. Light Blue top-tubes 3. Red or Speckled tubes 4. Green top tubes 5. Lavender top tubes 6. Gray top tubes |
| What color tube is used for DNA testing? | Yellow |
| Tests done in the blood bank require what color tube? | Red or Lavender or Pink |
| What is the CLSI Order of Draw? | 1. Yellow - Sterile 2. Light Blue top-Coagulation 3. Red - Serum 4. Red Tiger Top - SST 5. Light Green - Lithium Heparin 6. Dark Green - Sodium Heparin 7. Lavender - EDTA 8. Gray - Glycolytic inhibitor |
| A urine C & S is typically ordered to detect what? | U.T.I. |
| What hormone is detected in a positive urine pregnancy test? | HCG |
| Which test might require 'chain of custody?' | Drug Test |
| Which color tube is used for lead testing? | Tan |