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Order of Draw NHA

Patient Care Technician

QuestionAnswer
Blood Cultures or Yellow Top Additive: SPS 8-10 inversions, must be drawn 1st Dept: Microbiology
Light Blue Top Additive: Sodium Citrate 3-4 inversions Dept: Coagulation *Must be filled completely
Green Top Additive: Heparin 8-10 inversions Dept: Chemistry
Lavender Top Additive: EDTA 8-10 Inversions Dept: Hematology *Ammonia levels require a lavender top, and must be kept in an ice slurry after collected.
Pink Top Additive: EDTA 8-10 Inversions Dept: Hematology *Pink tops are used for blood bank specimens (blood type and matching for transfusion),
Gray Top Additive: Oxalate or Sodium Fluoride 8-10 Inversions Dept: Chemistry *Glucose tests, lactic acid Alcohol (ETOH) *Lactic acid tests must be kept in an ice slurry
Royal Blue Top Additive: EDTA or no additive Dept: Specialty *Certified to be free of trace elements, so can be used when testing blood for trace elements, toxicology, and nutritional tests.
Red Top Additive: Clot activator (plastic tubes), or no additive (glass tubes) Dept: Chemistry/Serology
Gold Top Additive: Clot activator and gel separator 5-8 inversions Dept: Chemistry, serology *Called serum separator tubes (SSTs), Do not use when testing for therapeutic drug levels
Orange Top Additive: Fastest acting clot activator Dept: chemistry/serology Called Stat Serum Tubes, used for testing that must be done very quickly. Hence “stat”
Created by: user-1910731
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