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DOULIN MICROSCOPY

Learning About Microscopy

questionAnswer
immersion oil gives the image contrast, brilliance, sharpness, and clarity.
Probably the most important instrument for many biologists is the ? microscope.
The most commonly used instrument for many biologists is a ? a light microscope.
The microscope most commonly in use in the microbiology laboratory is the ? compound microscope.
A compound microscope is one that uses a combination of lens systems: ( Objective lens system & Ocular or eyepiece lens system) . A compound microscope is one that uses a combination of lens systems : ( Objective lens system & Ocular or eyepiece lens system) .
The objective lenses are the lenses nearest the specimen or object The objective lenses are the lenses nearest the specimen or object
the ocular or eyepiece lenses are the ones nearest the eye. the ocular or eyepiece lenses are the ones nearest the eye.
The most common objectives used in a microbiology laboratory are the: 10x low power lens; the 45X high, dry power lens; and the 100x box oil immersion lens. The most common objectives used in a microbiology laboratory are the: 10x low power lens; the 45X high, dry power lens; and the 100x box oil immersion lens.
10x----dry power lens low power lens
45X---dry power lens high power lens
100x --- oil box oil immersion lens.
Care of the Microscope Always use two hands when carrying the microscope. Grasp the arm of the microscope with one hand and place the other hand under the base. Never tilt the microscope so the oculars could fall out. Place the microscope far enough from edge of workbench
Care Of Microscope The microscope should always be kept clean and clear of dust. It is a good idea to clean the lens systems (eyepiece, objectives, and condenser) before using the microscope. It is absolutely mandatory that the lenses be cleaned after each use.
Care Of Microscope Use special lens-paper when cleaning the lenses. Clean the oil immersion lens, last so as not to transfer any oil from the lens paper to another lens. A small amount of xylene on a clean piece of lens paper can be used to remove any excess immersion oil
When finished using the microscope: a) Clean lenses. b) Place the low power objective in the working position and rack down. c) Turn light control to lowest setting, then turn light off and unplug if removing the microscope from the workbench. d )Replace dust cover.
Use of the Microscope: materials are ? Microscope, Immersion oil, Lens-paper, 1/unit Microscope slides, Microscope cover-slips, Plastic pipette, culture , Wright's stained slide
The objective lens is, at its simplest, a very high powered magnifying glass The objective lens is, at its simplest, a very high powered magnifying glass
In most microscopes, the eyepiece is a compound lens, (blank)
Created by: TONYMILLER