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Phlebotomy Tubes
Basic/specialty tubes, order of draw, additives, specimen testing, department
| Term | Definition |
|---|---|
| Order of Draw | Yellow, blue, red, gold, green, lavender, gray |
| #1 Order of draw | Yellow |
| Yellow additive | SPS |
| Yellow alternate names | blood culture aerobic and anaerobic vials/bottles |
| Yellow mode of action | anticoagulant that does not inhibit bacterial growth |
| SPS | sodium polyanethole sulfonate |
| Yellow specimen testing | blood culture, fever of unknown origin, septicemia, bacteremia |
| BC | blood culture |
| FUO | fever of unknown origin |
| Yellow department | microbiology |
| #2 Order of draw | blue |
| Blue additive | Na citrate (sodium citrate) |
| Blue alternate names | light blue |
| Blue mode of action | anticoagulant that binds/blocks/inhibits calcium, preserves clotting factors |
| Blue department | coagulation |
| Blue specimen testing | coagulation studies, PT/INR, aPTT/PTT, fibrinogen, d-dimer |
| PT/INR | prothrombin time/protime; extrinsic |
| aPTT/PTT | partial thromboplastin time; intrinsic |
| Fibrinogen | circulates in the blood converts to fibrin to stop bleeding |
| D-dimer | test measures the level o d-dimer in the blood to check for blood clotting problems |
| BC test | yellow-SPS |
| FUO test | yellow-SPS |
| septicemia test | yellow-SPS |
| bacteremia test | yellow-SPS |
| PT/INR test | blue-citrate |
| aPTT/PTT test | blue-citrate |
| fibrinogen test | blue-citrate |
| d-dimer test | blue-citrate |
| #3 Order of Draw | red |
| Red additive | plain, no additive |
| Red mode of action | blood takes 30-60 minutes to clot |
| Red alternate names | plain red |
| Red department | chemistry, serology/immunology |
| Red specimen testing (chemistry) | routine chemistry tests for serum BMP, CMP, Lipid panel, Thyroid panel, Hepatic panel, Renal panel |
| Red specimen testing (RED ONLY chemistry) | CEA, CPK/CK, TSH, FSH, LH, Estrogen, PSA, Albumin, Calcium, Cholesterol, Bilirubin TDM (therapeutic drug monitoring) |
| Red specimen testing (serology/immunology) | HIV antibodies, HCG, ANA, Immunogobulins, RF, RPR |
| BMP | basic metabolic panel, red |
| CMP | complete metabolic panel, red |
| ... panel | chemistry testing |
| CEA | carcinoembryonic antigen, red |
| CPK/CK | creatine kinase, red |
| TSH | thyroid-stimulating hormone, red |
| FSH | follicle-stimulating hormone, red |
| LH | luteinizing hormone, red |
| PSA | prostate-specific antigens, red |
| TDM | therapeutic drug monitoring, red |
| HIV | human immunodeficiency virus, red |
| HCG | human chorionic gonadotropin, red |
| ANA | anti-nuclear antibody, red |
| RH | rheumatoid factor, red |
| RPR | rapid plasma reagin, red |
| #4 Order of Draw | Gold |
| Gold alternate names | Tiger top, SST, speckled red |
| SST | serum separator tube |
| Gold additive | clot activator, separator gel/thixotropic gel |
| Gold mode of action | blood clots in 15-30 minutes, the gel forms a barrier between the serum and the clot after centrifugation |
| Gold specimen testing | routine chemistry test for serum *NOT compatible with TDM testing |
| Gold department | chemistry |
| #5 Order of Draw | Green |
| Green additive | Na Heparin (sodium heparin) |
| Green mode of action | anticoagulant that binds/blocks/inhibits thrombin natural coagulant of the body |
| Green specimen testing | STAT chemistry tests for plasma ALT, AST, BUN, Electrolytes |
| Green department | chemistry |
| Green specimen testing (GREEN ONLY chemistry) | Ammonia |
| Green specimen testing, 2nd best choice | lactic acid/lactate, gray is the best choice |
| ALT | Alanine transaminase, green |
| AST | Aspartate transferase, green |
| BUN | blood urea nitrogen, green |
| #6 Order of Draw | Lavender |
| Lavender alternate names | purple |
| Lavender additive | EDTA (ethylenediamine tetraacetic acid) |
| Lavender mode of action | anticoagulant that binds/blocks/inhibits calcium anticoagulant that preserves the morphology of blood cells |
| Lavender department | hematology |
| Lavender specimen testing | hematology tests CBC, DIFF. C.T., Hgb, Hct, H&H, ESR, HgbA1c/A1c |
| CBC | complete blood count, lavender |
| DIFF. C.T. | differential, lavender |
| Hgb | hemoglobin, lavender |
| Hct | hematocrit, lavender |
| H&H | hemoglobin and hematocrit, lavender |
| ESR | erythrocyte sedimentation rate (2nd best choice lavender), black is best choice |
| HgbA1c | hemoglobin A1c, lavender |
| #7 Order of Draw | Gray |
| Gray additives | K oxalate (potassium oxalate), Na fluoride (sodium fluoride) |
| Gray mode of action | anticoagulant that binds/blocks/inhibits calcium, antiglycolytic/glycolytic inhibitor |
| Gray department | chemistry-glucose |
| Gray specimen testing | glucose: GTT, FBS, FBG Alcohol/ETOH Lactic acid/lactate (best choice) |
| GTT | glucose tolerance test |
| FBS | fasting blood sugar |
| FBG | fasting blood glucose |
| Specialty Tubes (8) | pink, royal blue, light green, tan, black, orange, pearl white, yellow-ACD |
| Pink additive | EDTA |
| Pink department | immunohematology/blood bank |
| Pink specimen testing | ABO blood typing/ABO group, cross matching/x-matching, type and cross/screen, antibody screen/ABY, Rh Typing |
| Royal blue alternate name | dark blue |
| Royal blue additive | clot activator (serum specimen) OR EDTA (plasma specimen) |
| Royal blue department | special chemistry or toxicology |
| Royal blue specimen testing | trace elements/trace minerals (mercury, aluminum, zinc) toxicology/drug screening heavy metals screening nutrition studies |
| Light green alternate names | PST, green-black |
| Light green additive | Li Heparin (lithium heparin), separator gel |
| Light green mode of action | the gel forms a barrier between the plasma and the blood cells after centrifugation |
| Light green specimen testing | STAT chemistry testing for plasma *NOT compatible with TDM testing |
| Light green department | chemistry |
| Tan additive | EDTA |
| Tan specimen testing | Lead |
| Tan department | special testing |
| Black additive | citrate |
| Black specimen testing | ESR (best choice) |
| Black department | hematology |
| Orange alternate names | RST |
| RST | rapid serum tube |
| Orange additive | Thrombin, separator gel |
| Orange mode of action | blood clots within 5 minutes |
| Orange specimen testing | STAT serum testing |
| Orange department | chemistry |
| Pearl white alternate name | PPT |
| PPT | plasma preparation tube |
| Pearl white additive | EDTA, separator gel |
| Pearl white specimen testing | PCR testing |
| PCR | polymerase chain reaction, pearl white |
| Yellow ACD | acid citrate dextrose |
| Yellow ACD mode of action | anticoagulant |
| Yellow ACD | DNA/genetic testing Paternity testing HLA phenotyping |
| Yellow ACD deparment | genetics |
| 1 hour- GTT | 1 gray tube, 50g glucola, draw after 1 hour |
| GTT instructions | No food, no drinks except sips of water. Patient needs to remain in the facility and needs to be on time at each draw interval. No exercise or strenuous activity. |
| 2 hour- GTT | 3 gray tubes, 75g glucola, draw fasting tube baseline before, draw at 1hr and 2hr after |
| 3 hour- GTT | 4 gray tubes, 100g glucola, draw fasting tube baseline before, draw at 1 hr, 2 hr, and 3 hr after |
| 2 hour- Postprandial (after meal) | 2 gray tubes, draw fasting tube as baseline before, collect postprandial sample 2 hours after completion of a meal |
| BC testing site preparation | cleanse site with alcohol: chlorhexidine, benzalkonium, tincture of iodine |
| BC site draw and timing | all BC are drawn at 2 different sites and 15 minutes apart unless mentioned otherwise |
| 24 hour urine testing | best to start early morning with an empty bladder. keep refrigerated |
| FOB | fecal occult blood test |
| FOB testing | colon cancer screening, 3 days prior avoid eating red meat, vitamin C, aspirin. record 3 separate bowel movements |
| O&P | ova and parasite testing |
| O&P testing | 3 vials, scoop fecal matter into vials to indicated "fill line" |
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