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Order of draw
Phlebotomy tube order of draw and additives
| Term | Definition |
|---|---|
| What is the standard order of draw? | Blood culture/Yellow (SPS)-Lt blue-red-SST-PST-green-lavender-grey |
| What additive is in lavender/pink tubes? What dept? | Spray coated K2 EDTA (Ethylenediaminetetraacetic acid) Hematology & Blood bank |
| What additive is in light green tubes? dark green? what dept? | Lithium heparin, sodium heparin - Chemistry |
| What tube can be used in place of culture bottles? | Yellow SPS |
| What does SST/PST stand for? | Serum separator tube, Plasma separator tube |
| What additive is in light blue tubes? What dept? | Sodium citrate - Coagulation |
| What is the difference between an SST and a PST? | One separates plasma, one separates serum. PST can be used STAT since clotting is not required. |
| If a small amount of blood is obtained for cultures, which bottle should be filled? | Aerobic |
| What is the ratio for Lt blue tubes? | 9:1, 9 parts blood to 1 part additive |
| What are the additives in the red tube? What dept? | Glass-none, plastic-silica coated (clot activator) - Chemistry, Immunology & Serology, Blood Bank |
| What are the additives in the red tiger top? What dept? | Silica coated (clot activator) and thixotropic gel - Chemistry |
| What tube has the same additives as the red tiger top? | Gold |
| What is the general clotting time for SST tubes? | 30 minutes |
| What color top is the rapid serum separator (RST)? | Orange - thrombin additive |
| The only 2 tubes with thrombin as their additive are? | Orange and grey/yellow tiger |
| What is the yellow top ACD used for? | HLA typing, DNA and paternity testing |
| What additive is in the white top tube? | K2 EDTA and thixotropic gel |
| What is the white/pearl top tube used for? | Molecular/PCR testing and bDNA testing (branched) |
| What additives are in grey top tubes? What dept? | Sodium fluoride and/or potassium oxalate - Chemistry |
| What tests can be drawn in a grey top tube? | Glucose (primarily), blood alcohol (ETOH), Hemoglobin A1C, lactic-acid |
| What is a standard mnemonic for the order of draw? | Stop Light Red Stay Put Green Light Go |
| What tube contains liquid K3 EDTA? | Glass Lavender |
| What tube is used for lead tests only? and what additive is in it? | Tan - K2 EDTA |
| What are royal blue top tubes used for? | Trace-element, toxicology, and nutritional chemistry |
| What additives are in the royal blue top tube? | Silica coated (clot activator) - K2 EDTA - sodium heparin |
| What are blood cultures used for? What dept? | Find bacteria and other germs in the blood - Microbiology |
| What does EDTA, Sodium Citrate, and sodium fluoride do? | Prevents blood from clotting by binding calcium |
| What do lithium/sodium heparin do? | Inactivate thrombin and thromboplastin (platelets) |
| What tube should never be used for hematology or lithium testing? | Green |
| what does ACD do in the yellow top? | Inhibits clotting but maintains RBC viability |
| What does SPS do in the yellow top? | Preserves specimens for cultures |
| What does sodium Fluoride do? | Inhibits glycolic action, preserves sugar from breaking down for up to 3 days |
| What tube is inverted 3-4 times? | Light Blue |
| What tube is inverted 8 times? | Yellow, green, lavender, grey |
| What tube is inverted 5 times? | Red tiger |
| How many times is a plain red inverted? | None |
Created by:
ahopper
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