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Phlebotomy Tests
Comments
| Test | Comment |
|---|---|
| Lactate (lactic acid) (Lact) | Analyze in 15 minutes; draw without tourniquet; place in an ice slurry when collecting in an arterial blood gas syringe; however, follow facility protocol |
| Blood culture | Aseptic technique; do not refrigerate |
| Blood group and type (ABO and Rh) | Blood bank ID |
| Antibody identification (ID)/screen | Blood bank ID |
| Type and screen | Blood bank ID |
| Crossmatch | Blood bank ID remains on 72 hours |
| Cryoglobulin | Collect in prewarmed tube and keep at 37°C |
| Calcium | Deliver to the laboratory immediately; refrigerate |
| Ethanol/alcohol (ETOH) | Do not open tube until testing; may require chain of custody; only use nonalcohol solution for skin cleansing |
| Electrolytes (Lytes) Na, K, Cl, CO2 | Do not refrigerate specimen |
| Carbon monoxide (CO) | Do not underfill; refrigerate immediately |
| Aluminum (Al) | Do not use gel |
| Lithium (Li) | Draw 12 hours post dose |
| Fasting blood sugar (FBS) | Ensure patient has fasted for 8 hours before blood collection |
| C-peptide | Ensure patient has fasted; avoid hemolysis |
| Adrenocorticotropic hormone (ACTH) | Freeze plasma |
| Antidiuretic hormone (ADH) | Freeze plasma |
| Antithrombin III | Freeze plasma immediately |
| Viral load | Freeze plasma immediately |
| Plasminogen | Freeze plasma immediately |
| Acid phosphatase | Freeze serum |
| Partial thromboplastin time (PTT); activated partial thromboplastin time (APTT) | Full tube; nonheparinized samples are stable at RT up to 4 hours; heparinized samples must be centrifuged within 1 hour and are stable up to 4 hours |
| Prothrombin time (PT) | Full tube; stable at RT up to 24 hours |
| Differential (Diff) | Make blood smear within 1 hour of collection |
| Cold agglutinins | Must be kept at 37°C; no gel barrier tubes |
| Carbamazepine (Tegretol) | No gel barrier tubes |
| Antibiotic assays (amikacin, gentamicin, theophylline, tobramycin, vancomycin) | No gel barrier tubes |
| Drug screen | No gel barrier tubes |
| Cyclosporine | No gel barrier tubes |
| Salicylate (aspirin) | No gel barrier tubes |
| Valproic acid (Depakote) | No gel barrier tubes |
| Therapeutic drugs (digoxin, theophylline [theo], phenobarbital, phenytoin [Pheny], carbamazepine [Carb], valproic acid [val ac]) | No gel barrier tubes; centrifuge and separate within 1 hour |
| Vitamin A | No gel barrier tubes; ensure patient has fasted |
| Vitamin B6 | No gel barrier tubes; ensure patient has fasted; protect from light |
| Calcitonin | No gel barrier tubes; freeze serum immediately |
| Aldosterone | Patient should be lying down for at least 30 minutes before blood collection |
| Parathyroid hormone (PTH) | Place in an ice slurry |
| Homocysteine (Hcy) | Place in an ice slurry |
| Angiotensin-converting enzyme (ACE) | Place in an ice slurry |
| Vitamin B12 | Protect from light |
| Carotene, beta | Protect from light |
| Bilirubin, total and direct (Bili) | Protect from light |
| Porphyrins | Protect from light |
| Cancer antigen (CA-125) | Refrigerate |
| Antistreptolysin O (ASO) titer | Refrigerate immediately or freeze serum |
| Ammonia (NH3) | Send in an ice slurry |
| pH | Send on ice slurry |
| Chromium (Cr) | Separate and refrigerate immediately |
| Cortisol | Serum only; timed specimen (a.m.) |
| Brain natriuretic peptide (BNP) | Stable for 4 hours |
| MI panel (CK isoenzyme [CK-MB], myoglobin [Myo], troponin [Tn]) | Stable for 4 hours |
| Gastrin | Transport in an ice slurry |
| Erythrocyte sedimentation rate (ESR) | Tube must be filled at least half-full |
| Fibrinogen | Tube must be full |
| International normalized ratio (INR) | Tube must be full |
| Factor assays | Tube must be full |
| Ionized calcium (iCA2+) | Tube must be full |
| D-Dimer (D-DI) | Tube must be full (stable for 4 hours) |
| Fibrin degradation product (FDP) | Tube will only fill to 2 mL and should clot immediately |
Created by:
cvis
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