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BCLT 4-4 hCG Tests

hCG pregnancy hormone, prod by placenta, detectable 1 week after implantation
enzyme immunoassay - new method aka chromatographic immunoassay
qualitative test urine
quantitative test serum
CLIA waived only with urine
chromatographic immunoassay principle anti hCG antibodies >> attach color particles >> coat membrane strip >> complex (color change) or no complex forms
1st morning urine best b/c most concentrated
test asap or fridge >> up to 24-48 hours
read results in 5 min exactly, on time
who reports results RN or doc, not phleb!
qualitative enzyme immunoassay >> followed up by quantitative serum hCG
other conditions cause pos hCG test results tumors: breast, ovarian, testicular; trophoblastic disease
pos hCG urine or serum test >> not to used to diagnose preg unless 1 and 2 1. other clinical findings support 2. other conditions have been ruled out



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