Help
Options
Didn't know it?
click below
click below
Knew it?
click below
click below
Don't Know
Remaining cards (0)
Know
retry
shuffle
restart
0:00
Embed Code - If you would like this activity on your web page, copy the script below and paste it into your web page.
Normal Size Small Size show me how
Normal Size Small Size show me how
MicroBiology
Bacterial Growth Requirements and Gram Staining
| Question | Answer |
|---|---|
| What ingredient converts liquid to solid medium? | Agar |
| Selective Media | Inhibits growth of certain organisms and permits growth of others |
| Differential Media | Gives colonies of a particular organism a distinct appearance (makes them all look different) |
| Enrichment Media | Contains complexed materials that certain fastidious organisms require to grow |
| Nutritive Media | Grows a wide number of bacteria |
| Bacterial Colony | Macroscopic growth |
| Streak Plate Method | Isolated Colonies |
| Pure Culture | Single Species or kind |
| Crystal Violet | Primary stain (applied first) |
| Iodine | Mordant |
| Acetone/Alcohol | Decolorizer |
| Safranin | Counter Stain |
| What color is Gram Stain | Purple--resist decolorization and keep the primary stain |
| What color is Gram Negative | Red--Easily decolorized - take up counter stain |
| What do the first and second names of microbes denote | Genus Species |
| How are the first and second names written | Genus - Capital Species - lower |
| What are characteristics that bacterial cells have in common | Rigid cell wall,Flagella, Unicellular, Binary Fission, DNA & RNA |
| Describe - Cell Wall | Varies in composition, accounts for different Gram stain |
| Describe - Plasma Membrane | Regulates passage of material in and out of cell walls |
| Describe - Nucleoid | Genetic mat'l - DNA & RNA |
| Describe - Capsule | Sticky, gummy6 envelope secreted by some bacteria. Protects from phagocytosis |
| Describe - Flagella | Aids in locomotion (movement) |
| Describe Spore/Endospore | Thick wall, oval body resistant to (adverse conditions) killing or being killed |
| Define - Aerobe | (Humans) - will grow in the presence of free oxygen |
| Describe - Anaerobe | (Fish) - cannot grow in the presence of free oxygen (cannot tolerate) |
| Describe Facultative Anaerobe | Basically an aerobe but can grow in the absence of free oxygen (survive a wide range of conditions - hard to kill off) |
| Define - Obligate Anaerobe | Can grow only in the complete absence of free oxygen (very difficult to grow - fussy) |
| What pH is best for bacterial growth | pH 7 - can tolerate a narrow range from 6 - 8 |
| What is the best time to use bacterial cultures for lab studies` | 18 - 24 hours |
| What are exotoxins | Exit - some bacteria make poison called toxins |
| What are endotoxins | Release when the cell is destroyed, because they are bound to the cell wall |
| What is the Gram reaction of bacteria that produce exotoxins? | Gram Positive - Diptheria, Tetnas, Gangreen |
| What is the Gram reaction of bacteria that produce endotoxins? | Gram Negative - Salmonella, Cholera |
| Exotoxins | Gram Positive |
| Endotoxins | Gram Negative |
Created by:
wenchie040
Popular Medical sets