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# ADV.D04, Ch.8 Eqp.

# ADV.D04, Ch.8 Tubes & Equipment

EDTA tubes lavender, royal blue, tan, white, pink
Heparin tubes light green with gel and green without gel. Both are used for STAT chemistry tests. Heparin controls potassium (K+) levels.
Thrombin tubes Orange or Gray/yellow. Blood clots quickly. Reduces "wait time" before testing. Used for serum chemistry tests.
Gray tubes Have calcium oxalate and sodium fluoride. Sodium fluoride preserves the glucose. Used for blood sugar (glucose) tests.
SPS tubes Sodium polyanethanol sulfonate Blood culture transport tube. Keeps most aerobes and anaerobes alive until blood can be put into culture bottles.
Light Blue tube Used for coagulation (blood clotting) tests. Mostly used for PT (prothrombin time) and PTT (partial thromboplastin time).
Nonadditive tubes Gold top is a safety top type. Red/Gray is a gel tube (SST). Red plain tube is for immunology and immunohematology.
clot activator glass / silicone beads sprayed inside the tube.
thixotropic gel separation gel used in red/gray serum tubes. Also called a polymer gel. It has a specific gravity lighter than water. It separates blood cells from the serum
serum the liquid part of clotted blood
plasma the liquid part of unclotted blood
glycolytic inhibitor anything that can stop glycolysis (breaking down sugar). Fluoride preparations (gray tubes) do this.
gauge A number that represents the width of a needle
whole blood Blood with both the liquid and cells together.
See appendix 7 Good reference for what tube to use for a lab test.
anticoagulant chemical additive that prevents blood from clotting.
oxalate, citrate, EDTA, heparin removes calcium from the clotting reactions and blood does not clot
effect of heparin on RBCs Alters the wright's stain for peripheral smears. Also known to distort the shape of RBC.
aseptic without bacterial contamination
anaerobic bottle designed for growing anaerobic bacteria
anaerobic "without oxygen". A type of bacteria that needs CO2 and not oxygen (O2). They are killed by oxygen (O2).
aerobic A type of bacteria that needs oxygen to live.
blue top tube requirements Must be full. Test cannot be done if tube is underfilled.
length of time for blood to completely clot in a plain red top tube. 30 minutes
SST serum separation tube:
plain red top tube used for blood bank (immunohematology) and immunology departments. This has no polymer gel and no clot activator.
Green top tubes Tube may have Sodium heparin (Na), Ammonium heparin (NH4), or Lithium heparin (Li) anticoagulants. Used for STAT chemistry tests. Also controls potassium level. Clotted blood contains a higher level of potassium.
Lavender EDTA tube Used for hematology: Red cell count, White cell count , Platelet count, RBC indices : MCH, MCHC, MCV
How many gentle inversions (mixing) do you do for blood tubes 8 to 10 times for most tubes. 4x for small light blue tubes
Gray top tubes Contains fluoride. fluoride is an antiglycolytic agent. This means it prevents the red cells from using the glucose in the blood.
Royal blue top tubes with or without EDTA. Used for finding trace elements and some nutritional elements that exist in small amounts in the blood.
Tan top tubes Has EDTA. Used for lead determinations
what is the correct order of draw for microtainers 1. edta 2. other additive tubes 3. non additive tubes
what is the correct order of draw for vacuum tubes 1. Yellow (SPS) 2. Light blue (citrate) 3. Serum tubes 4. Green tops 5. EDTA (lavender, pink, black, royal blue) 6. gray top (fluoride)
Yellow top with black band is for... Blood cultures and the tube has SPS.
Yellow top for cell preservation is the ACD tube. Acidified Citrate Dextrose.
light blue coagulation tube has... sodium citrate
Plain red top tube has... no additives
gold safety top tube has gel and clot activator
The tiger top tube is also called the... SST (serum separator tube)
A green top with gel has... sodium heparin and thixotropic gel
light b lue top tube has... sodium citrate. Tube must be filled completely or the tests cannot be done.
Created by: rjmtoss