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Microbio: Stains

USMLE Step 1

StainOrganism(s)
Ziehl Neelsen acid fast bacteria, i.e. Mycobacterium tuberculosis
India Ink Cryptococcus neoformans
Giemsa Borrelia, Plasmodium, Trypanosomes, Chlamydia also blood smears
Periodic Acid-Schiff (PAS) tests for glycogen & mucopolysaccharides, used to dx Whipple's disease from the bacterium Tropheryma whipplei
Prussian blue blue dye test to detect Fe
Congo red stains amyloid red & causes amyloid to take up apple-green birefringence when viewed under polarized light microscope
Osmic acid fixative & stain for EM
Gram stain + for thick peptidoglycan (blue-violet) vs. - for thin peptidoglycan (pink-red)
Created by: jerrica_08
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