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Phleb 12 + 13

Phlebotomy Essentials 5th Edition Chapters 12 13

Why is specimen handling so important? ALL
Proper specimen handling begins When test is ordered
You are the only phlebotomist on duty. A physician orders a test you’re unfamiliar with. What do you do? Consult the user manual.
Number of inversions depends on Presence or absence of additive
Inadequate mixing of tubes with anticoagulant could lead to Microclots
Tube that needs no mixing Non-additive
Why must you transport tubes with stopper up? ALL
Specimen transported via courier, aircraft, mail, must follow guidelines by (FAA, OSHA, DOT) ALL
Which of the following actions will compromise the quality of a specimen? Partially filling a SST tube
Analyte broken down by light Bilirubin
Some specimens require cooling to Slow down metabolic processes
Chilling can cause erroneous test results for which analyst Potassium
What would be transported in ice slurry? Ammonia
What is the best way to chill a specimen? Ice Slurry
How should a Cryofibrinogen specimen be transported? Warm - 37°C heat block
Why would you wrap a specimen tube in aluminum foil? To protect from light
Specimen must be transported at near normal body temp, which is 37°C
According to CLSI the maximum time limit for separating serum or plasma from cells is 2 hours
Separator gels prevent glycolysis when? After being centrifuged
Glucose in a sodium fluoride tube can be stored at room temp for how long? 24 hours
What type of test takes priority? STAT (STAT-ER)
All of the following are centrifuged, except CBC in lavender top tube
What protective equipment is required when processing specimens? ALL
What would be a reason to reject specimen for analysis? ALL
A reason to reject a specimen would include all of the following, except Icteric bilirubin and lipemic fasting glucose
Aliquot Portion of specimen being tested
Specimen rejected if tube not filled completely until vacuum is exhausted PT (ProTime)
Tests performed on plasma are Collected in anticoagulant tubes
It is important to note the type of heparin in the collection tube because Some types of heparin can affect test results.
Non additive specimen is spun in a centrifuge to obtain Serum
Serum will take longer to clot ALL
Test most affected by contamination of a drop of perspiration falling into it Electrolytes
Gloves that contain powder Source of contamination
Minimum pre-centrifugation time for a specimen drawn in SST tube 15 mins
Repeated centrifugation of a specimen can ALL
Proper centrifuge operation Balance
Special information when labeling a non-blood specimen Type and source
What type of specimen must be handled and analyzed STAT CSF (cerebrospinal fluid)
Most frequently analyzed non-blood specimen Urine
What can happen to urine components if not processed in a timely fashion? ALL
What specimen preferred for most urine tests? First morning urine
Routine urinalysis specimens that cannot be analyzed within 2 hours require Refrigeration
Routine urinalysis typically includes ALL
Most likely reason a Culture + Sensitivity be ordered? To diagnose UTI
Urine cytology studies look for presence of Abnormal cells
Suspected pregnancy can be confirmed by testing urine for this hormone HCG
What type of specimen preferred for pregnancy testing? First morning urine
What type of specimen preferred routine analysis? Random
Urine specimen that is most concentrated First morning urine
Serial urine specimens collected at specific times for C & S GTT (glucose tolerance test)
Which urine spec is obtained by inserting a sterile needle directly into the urinary bladder and aspirating urine? Suprapubic
Procedure for collecting 24 hr urine specimen Void first morning urine into toilet, start timing, collect all following including next am specimen
Which urine test requires midstream catch specimen? C+S (culture and sensitivity)
Test performed on amniotic fluid AFP (alpha fetoprotein)
Amniotic fluid comes from where? Sac surrounding fetus
Spinal fluid analysis useful in diagnosing Meningitis
Test requires IV administration of histamine or pentagastrin Gastric analysis
NP (nasopharyngeal) culture swab sometimes collected to detect Whooping cough
Saliva can be tested to ALL
One reason semen analysis is performed Assess fertility
Which of the following fluids is obtained through lumbar puncture? Spinal
Serous fluid includes ALL
Fluid from joint cavities Synovial
Ascites Excess fluid in peritoneal cavity
Sputum Phlegm
Sputum is collected in the diagnosis and monitoring of TB
Test to diagnose CF (Cystic Fibrosis) Sweat chloride (Iontophoresis)
Synovial fluid collected in what tube ALL
Synovial fluid used to identify ALL
The process of Iontophoresis is used to collect Sweat
Marrow aspirated from Iliac crest (hip)
Marrow studied to identify Blood disorders
Breath specimens can be used to detect H. Pylori
Refrigerated stool specimen would be acceptable for all except O + P (ova & parasites)
Guiac test detects Occult blood
Chronic drug use can be detected in what type of sample? Hair
Type of specimen required for rapid strep test Throat swab
Type of specimen required for biopsy Tissue
Military time Examples: 8:00am= 0800 “0 eight-hundred hours” 10:15am= 1015 “ten fifteen” 1:30pm= 1330 “thirteen thirty”
Bleach dilution 10% dilution of bleach (5.25% sodium hypochlorite) 1:10 ratio of bleach to water 1 part bleach to 9 parts water 10mL bleach to 90mL water
Created by: 708240517



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