Pigment & mineral Test
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| A. Fixative: alcohol preferred, 10% NBF okB. melanin, argentaffin granules - black
nuclei - pink
overstaining gives gray background & loss of contrastC. Reticulin, Holmes,
Bielschowsky, Warthin-Starry,
Dieterle, Steiner & Steiner,
Grimelius, Churukian-SchenkD. Formed as a result of chemical action -
fixation, processing,
usually on tissue, not w/i cell,
e.g. formalin, mercury, chrome pigmentsE. Fe2+, toxic, not normally stored,
Turnbull blueF. Demo argyrophil granules in neurosecretory tumors,
also stains argentaffin substancesG. Greenish bile pigment,
prod'd from destruction of rbc,
liver red. to bilirubin - remove from circulation,
abnormal accumulation - jaundice, pigment in bile canaliculi, cytoplasm of Kupffer cells & hepatocytesH. working Rhodanine w/ sodium acetate-formalin
Mayer hematoxylin w/ dH2O
boraxI. working silver solution
reducing solution
nuclear-fast redJ. working methenamine-silver nitrate
sodium thiosulfate
light green w/ acetic acidK. acetic acid-acetate buffer pH 5.6
silver nitrate 1%
dH2OL. argyophil granules, argentaffin substances - black
nuclei - red
background - yellow-brown M. Disease caused by excessive absorption of dietary iron,
excessive hemosiderin deposits in liver, pancreas,
damaging to tissueN. Fixative: alcohol or 10% NBF
QC: section w/ Fe2+O. ID calcium in tissue,
reaction product is birefringentP. Demo urates,
may demo large calcium depositsQ. Demo argentaffin substances, e.g. melanin,
argentaffin granules of carcinoid tumors,
some neurosecretory granules,
demos other red'ing substances (formalin pigment)R. Fixative: abs. alcohol
QC: section w/ uratesS. Urate crytals (gouty tophi),
chronic gout may deposit in soft tissue too,
sol. in water, alcoholic fix.,
birefringent w/ polarizing,
demo w/ argentaffin rxnT. Primary chromate fixatives,
e.g. Orth |
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