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Pigment & mineral Test

Enter the letter for the matching Answer
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1.
Hemoglobin
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2.
Prussian blue stain solutions
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3.
Hemosiderin
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4.
Artifact pigment
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5.
Endogenous hematogenous pigments
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6.
van Gieson solution
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7.
Asbestos fibers
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8.
Churukian-Schenk method results
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9.
Fontana-Masson & microwave Fontana-Masson stains facts
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10.
Biliverdin
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11.
Mayer mucicarmine solution
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12.
Working Rhodanine solution
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13.
Lipofuscin pigment
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14.
Gomori methenamine-silver method purpose
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15.
von Kossa stain solutions
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16.
Rhodanine method results
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17.
Alizarin Red S stain purpose
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18.
Microwave Churukian-Schenk method results
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19.
Stained starch granules show:
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20.
Microwave Rhodanine method solutions
A.
argyrophil & argentaffin cells - black nuclei - orange to red background - light yellow-orange
B.
From blood, e.g. hemoglobin, hemosiderin, biliverdin
C.
Fixative: 10% NBF, avoid alcohol QC: skin for melanin, small intestine or appendix for argentaffin granules, Melanoma, tumor w/ argentaffin granules, chemically clean glassware, nonmetallic instruments
D.
A characteristic Maltese cross configuration when polarized, may be introduced from talcum powder of gloves
E.
argyophil granules, argentaffin substances - black nuclei - red background - yellow-brown
F.
copper - bright red to red yellow nuclei - light blue If copper conc. low, stain may fade after coverslipping
G.
working Rhodanine w/ sodium acetate-formalin Mayer hematoxylin w/ dH2O borax
H.
acid fuchsin picric acid
I.
Formed as a result of chemical action - fixation, processing, usually on tissue, not w/i cell, e.g. formalin, mercury, chrome pigments
J.
"Wear & tear" pigment, yellow-brown, in more permanent cells (heart, liver, neurons)
K.
ID calcium in tissue, reaction product is birefringent
L.
carmine alum lake aluminum hydroxide anhy. aluminum chloride 50% ethanol 25% ethanol
M.
Demo urates, may demo large calcium deposits
N.
potassium ferrocyanide 2% (bind Fe3+) HCl 2% (acidify) nuclear-fast red
O.
Birefringent, magnesium silicate, in body fibers coated w/ iron-containing protein, known as asbestos bodies, lose birefringence demo w/ Prussian blue
P.
Yellow to brown pigment, stored iron (Fe2+), usu. in marrow, pathologic elsewhere (liver), differentiate from other yellow-brown pigments w/ Prussian blue rxn
Q.
Greenish bile pigment, prod'd from destruction of rbc, liver red. to bilirubin - remove from circulation, abnormal accumulation - jaundice, pigment in bile canaliculi, cytoplasm of Kupffer cells & hepatocytes
R.
5-(p-dimethylamino-benyzlidine) rhodanine abs. ethanol dH2O filter
S.
silver nitrate sunlight sodium thiosulfate nuclear-fast red
T.
Conjugated protein, anionic, stains w/ acid dyes (eosin), hemoglobin breaks into globin protein & heme, heme splits into iron & biliverdin
Type the Answer that corresponds to the displayed Question.
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21.
Paneth cell granules are destroyed by?
Type the Question that corresponds to the displayed Answer.
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22.
A substance that has enough color to be visible w/o any further staining
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23.
Fouchet reagent van Gieson
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24.
Indicate reducing substances - will red. ferric ions, stains melanin, argentaffin granules, formalin pigment, mostly replaced by IHC
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25.
QC: argyrophil-positive carcinoid tumor preferred, can use small intestine, chemically clean glassware, nonmetallic forceps
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26.
reducing/argentaffin substances - blue-green goblet cells, mucin - rose background - yellow-green (less better)
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27.
Fe3+, normally present in tissue, usu. bound to protein (hemosiderin), in marrow, spleen, decaying, hemorrhagic material, excess - hemachromatosis, Prussian blue
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28.
Fe2+, toxic, not normally stored, Turnbull blue
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29.
Is a reducing substance, may give positive rxn to Schmorl & Fontana-Masson
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30.
Demo argyrophil granules in neurosecretory tumors, also stains argentaffin substances

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