Pigment & mineral Test
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| A. argyrophil & argentaffin cells - black
nuclei - orange to red
background - light yellow-orangeB. From blood, e.g. hemoglobin, hemosiderin, biliverdinC. Fixative: 10% NBF, avoid alcohol
QC: skin for melanin,
small intestine or appendix for argentaffin granules,
Melanoma, tumor w/ argentaffin granules,
chemically clean glassware, nonmetallic instrumentsD. A characteristic Maltese cross configuration when polarized,
may be introduced from talcum powder of glovesE. argyophil granules, argentaffin substances - black
nuclei - red
background - yellow-brown F. copper - bright red to red yellow
nuclei - light blue
If copper conc. low, stain may fade after coverslippingG. working Rhodanine w/ sodium acetate-formalin
Mayer hematoxylin w/ dH2O
boraxH. acid fuchsin
picric acidI. Formed as a result of chemical action -
fixation, processing,
usually on tissue, not w/i cell,
e.g. formalin, mercury, chrome pigmentsJ. "Wear & tear" pigment,
yellow-brown,
in more permanent cells (heart, liver, neurons)K. ID calcium in tissue,
reaction product is birefringentL. carmine
alum lake
aluminum hydroxide
anhy. aluminum chloride
50% ethanol
25% ethanolM. Demo urates,
may demo large calcium depositsN. potassium ferrocyanide 2% (bind Fe3+)
HCl 2% (acidify)
nuclear-fast redO. Birefringent, magnesium silicate,
in body fibers coated w/ iron-containing
protein, known as asbestos bodies,
lose birefringence demo w/ Prussian blueP. Yellow to brown pigment,
stored iron (Fe2+),
usu. in marrow, pathologic elsewhere (liver),
differentiate from other yellow-brown pigments
w/ Prussian blue rxnQ. Greenish bile pigment,
prod'd from destruction of rbc,
liver red. to bilirubin - remove from circulation,
abnormal accumulation - jaundice, pigment in bile canaliculi, cytoplasm of Kupffer cells & hepatocytesR. 5-(p-dimethylamino-benyzlidine) rhodanine
abs. ethanol
dH2O
filterS. silver nitrate
sunlight
sodium thiosulfate
nuclear-fast redT. Conjugated protein,
anionic, stains w/ acid dyes (eosin),
hemoglobin breaks into globin protein & heme,
heme splits into iron & biliverdin |
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