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Pigment & mineral Test

Enter the letter for the matching Answer
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1.
Grimelius stain purpose
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2.
Microwave Rhodanine method solutions
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3.
Gomori methenamine-silver method purpose
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4.
Hemochromatosis
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5.
Alizarin Red S stain purpose
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6.
Grimelius stain solutions
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7.
Fontana-Masson & microwave Fontana-Masson stains purpose
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8.
Ferrous ions
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9.
Turnball blue stain facts
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10.
Fontana-Masson stain results
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11.
Gomori methenamine-silver method solutions
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12.
Artifact pigment
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13.
Churukian-Schenk method results
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14.
Engodenous deposits
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15.
Argyrophil stains
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16.
Gomori methenamine-silver method facts
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17.
Best fixative for chromaffin granules?
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18.
Grimelius working silver solution
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19.
von Kossa stain facts
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20.
Biliverdin
A.
Fixative: alcohol preferred, 10% NBF ok
B.
melanin, argentaffin granules - black nuclei - pink overstaining gives gray background & loss of contrast
C.
Reticulin, Holmes, Bielschowsky, Warthin-Starry, Dieterle, Steiner & Steiner, Grimelius, Churukian-Schenk
D.
Formed as a result of chemical action - fixation, processing, usually on tissue, not w/i cell, e.g. formalin, mercury, chrome pigments
E.
Fe2+, toxic, not normally stored, Turnbull blue
F.
Demo argyrophil granules in neurosecretory tumors, also stains argentaffin substances
G.
Greenish bile pigment, prod'd from destruction of rbc, liver red. to bilirubin - remove from circulation, abnormal accumulation - jaundice, pigment in bile canaliculi, cytoplasm of Kupffer cells & hepatocytes
H.
working Rhodanine w/ sodium acetate-formalin Mayer hematoxylin w/ dH2O borax
I.
working silver solution reducing solution nuclear-fast red
J.
working methenamine-silver nitrate sodium thiosulfate light green w/ acetic acid
K.
acetic acid-acetate buffer pH 5.6 silver nitrate 1% dH2O
L.
argyophil granules, argentaffin substances - black nuclei - red background - yellow-brown
M.
Disease caused by excessive absorption of dietary iron, excessive hemosiderin deposits in liver, pancreas, damaging to tissue
N.
Fixative: alcohol or 10% NBF QC: section w/ Fe2+
O.
ID calcium in tissue, reaction product is birefringent
P.
Demo urates, may demo large calcium deposits
Q.
Demo argentaffin substances, e.g. melanin, argentaffin granules of carcinoid tumors, some neurosecretory granules, demos other red'ing substances (formalin pigment)
R.
Fixative: abs. alcohol QC: section w/ urates
S.
Urate crytals (gouty tophi), chronic gout may deposit in soft tissue too, sol. in water, alcoholic fix., birefringent w/ polarizing, demo w/ argentaffin rxn
T.
Primary chromate fixatives, e.g. Orth
Type the Question that corresponds to the displayed Answer.
incorrect
21.
reducing/argentaffin substances - blue-green goblet cells, mucin - rose background - yellow-green (less better)
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22.
Seen in lung & lymph node, resists bleaching & extration, insol. in conc'd sulfuric acid - use to differentiate
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23.
Fouchet reagent van Gieson
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24.
Is a reducing substance, may give positive rxn to Schmorl & Fontana-Masson
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25.
Gomori methenamine-silver, Grocott methenamine-silver, Fontana Masson, von Kossa
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26.
Indicate reducing substances - will red. ferric ions, stains melanin, argentaffin granules, formalin pigment, mostly replaced by IHC
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27.
Fe3+, normally present in tissue, usu. bound to protein (hemosiderin), in marrow, spleen, decaying, hemorrhagic material, excess - hemachromatosis, Prussian blue
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28.
QC: argyrophil-positive carcinoid tumor preferred, can use small intestine, chemically clean glassware, nonmetallic forceps
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29.
argentaffin & argyrophil granules - dark brown to black nuclei - red background - pale yellow-brown
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30.
Conjugated protein, anionic, stains w/ acid dyes (eosin), hemoglobin breaks into globin protein & heme, heme splits into iron & biliverdin

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