Staining
Quiz yourself by thinking what should be in
each of the black spaces below before clicking
on it to display the answer.
Help!
|
|
||||
|---|---|---|---|---|---|
| Artifact pigment | show 🗑
|
||||
| Exogenous pigment | show 🗑
|
||||
| Carbon pigment | show 🗑
|
||||
| show | Birefringent, magnesium silicate,
in body fibers coated w/ iron-containing
protein, known as asbestos bodies,
lose birefringence demo w/ Prussian blue
🗑
|
||||
| Endogenous hematogenous pigments | show 🗑
|
||||
| show | Conjugated protein,
anionic, stains w/ acid dyes (eosin),
hemoglobin breaks into globin protein & heme,
heme splits into iron & biliverdin
🗑
|
||||
| show | Yellow to brown pigment,
stored iron (Fe2+),
usu. in marrow, pathologic elsewhere (liver),
differentiate from other yellow-brown pigments
w/ Prussian blue rxn
🗑
|
||||
| show | Disease caused by excessive absorption of dietary iron,
excessive hemosiderin deposits in liver, pancreas,
damaging to tissue
🗑
|
||||
| Biliverdin | show 🗑
|
||||
| show | Similar to bilirubin, ox. to bilirubin,
formed as result of hemorrhage & red'd O2 tension
🗑
|
||||
| Endogenous nonhematogenous pigments | show 🗑
|
||||
| show | Formed by ox. tyrosine,
histochem. demo melanocytes,
bleach w/ H2O2, potassium permanganate & oxalic acid,
dissolved by strong alkali,
insol. weak acid, base, organic solvents,
argentaffin,
positive Schmorl
🗑
|
||||
| show | "Wear & tear" pigment,
yellow-brown,
in more permanent cells (heart, liver, neurons)
🗑
|
||||
| show | Brownish-yellow,
hepatocytes & macrophages of rats w/ cirrhosis,
rarely seen in humans
🗑
|
||||
| What stains for lipofuscin & ceroid pigments? | show 🗑
|
||||
| show | Urate crytals (gouty tophi),
chronic gout may deposit in soft tissue too,
sol. in water, alcoholic fix.,
birefringent w/ polarizing,
demo w/ argentaffin rxn
🗑
|
||||
| Minerals | show 🗑
|
||||
| Cytoplasmic granules | show 🗑
|
||||
| show | Have high uptake of amine-precursors & ability to decarboxylate
🗑
|
||||
| Best fixative for chromaffin granules? | show 🗑
|
||||
| show | Alcoholic fixatives
🗑
|
||||
| Paneth cell granules are destroyed by? | show 🗑
|
||||
| Prussian blue stain purpose | show 🗑
|
||||
| show | Fixative: alcohol or 10% NBF,
QC: section w/ Fe3+, don't want excess iron,
chemically clean glassware,
iron-free reagents
🗑
|
||||
| show | potassium ferrocyanide 2% (bind Fe3+)
HCl 2% (acidify)
nuclear-fast red
🗑
|
||||
| Prussian blue stain results | show 🗑
|
||||
| show | aluminum sulfate
nuclear-fast red
thymol (preservative)
dH2O
🗑
|
||||
| Fixation for iron demo: | show 🗑
|
||||
| show | Detect ferrous (Fe2+) iron,
ferrous iron v. toxic, not normally stored,
easily absorbed by intestine but converted to ferric,
used w/ Schmorl technique
🗑
|
||||
| show | Fixative: alcohol or 10% NBF
QC: section w/ Fe2+
🗑
|
||||
| Turnball blue stain solutions | show 🗑
|
||||
| Turnball blue stain results | show 🗑
|
||||
| Potassium ferricyanide staining solution | show 🗑
|
||||
| Schmorl technique purpose | show 🗑
|
||||
| Schmorl technique facts | show 🗑
|
||||
| Schmorl technique solutions | show 🗑
|
||||
| show | reducing/argentaffin substances - blue-green
goblet cells, mucin - rose
background - yellow-green (less better)
🗑
|
||||
| show | carmine
alum lake
aluminum hydroxide
anhy. aluminum chloride
50% ethanol
25% ethanol
🗑
|
||||
| Fontana-Masson & microwave Fontana-Masson stains purpose | show 🗑
|
||||
| show | Fixative: 10% NBF, avoid alcohol
QC: skin for melanin,
small intestine or appendix for argentaffin granules,
Melanoma, tumor w/ argentaffin granules,
chemically clean glassware, nonmetallic instruments
🗑
|
||||
| show | Fontana silver nitrate w/ NH4OH
gold chloride
sodium thiosulfate
nuclear-fast red
🗑
|
||||
| show | melanin, argentaffin granules - black
nuclei - pink
overstaining gives gray background & loss of contrast
🗑
|
||||
| Microwave Fontana-Masson stain solutions | show 🗑
|
||||
| show | argentaffin cell granules, chromaffin granules, melanin,
other argentaffin substances - black
nuclei - pink
🗑
|
||||
| show | Demo argyrophil granules in neurosecretory tumors,
also stains argentaffin substances
🗑
|
||||
| show | QC: argyrophil-positive carcinoid tumor preferred,
can use small intestine,
chemically clean glassware, nonmetallic forceps
🗑
|
||||
| show | working silver solution
reducing solution
nuclear-fast red
🗑
|
||||
| Grimelius stain results | show 🗑
|
||||
| Grimelius working silver solution | show 🗑
|
||||
| show | hydroquinone
sodium sulfite
dH2O
🗑
|
||||
| show | Stain w/ both Grimelius (argyophil) & Fontana-Masson (argentaffin) & compare
🗑
|
||||
| Churukian-Schenk & microwave Churukian-Schenk methods purpose | show 🗑
|
||||
| show | QC: argyrophil-positive carcinoid tumor preferred,
can use small intestine,
chemically clean glassware, nonmetallic forceps
🗑
|
||||
| show | acidified water pH 4.0 to 4.2
silver nitrate 0.5%
reducing solution
nuclear-fast red
🗑
|
||||
| Churukian-Schenk method results | show 🗑
|
||||
| show | citric acid-glycine soln.
silver nitrate 0.5%
reducing solution
nuclear-fast red
🗑
|
||||
| show | argyrophil & argentaffin cells - black
nuclei - orange to red
background - light yellow-orange
🗑
|
||||
| Gomori methenamine-silver method purpose | show 🗑
|
||||
| show | Fixative: abs. alcohol
QC: section w/ urates
🗑
|
||||
| show | working methenamine-silver nitrate
sodium thiosulfate
light green w/ acetic acid
🗑
|
||||
| show | urates - black
background - green
🗑
|
||||
| Bile stain purpose | show 🗑
|
||||
| Bile stain solutions | show 🗑
|
||||
| show | bile, bilirubin - emerald green to olive drab
background - yellow
🗑
|
||||
| Fouchet reagent solution | show 🗑
|
||||
| show | acid fuchsin
picric acid
🗑
|
||||
| show | ID calcium in tissue,
detects anions bound to calcium, not the calcium itself
🗑
|
||||
| show | Fixative: alcohol preferred, 10% NBF ok
🗑
|
||||
| von Kossa stain solutions | show 🗑
|
||||
| von Kossa stain results | show 🗑
|
||||
| Alizarin Red S stain purpose | show 🗑
|
||||
| show | Fixative: alcoholic formalin or 10% NBF
🗑
|
||||
| show | Alizarin red S (chelate w/ calcium)
dH2O
NH4OH to pH 4.1 to 4.3 - critical
🗑
|
||||
| show | calcium deposits - orange-red
🗑
|
||||
| Rhodanine & microwave Rhodanine methods purpose | show 🗑
|
||||
| Rhodanine & microwave Rhondanine methods facts | show 🗑
|
||||
| show | working rhodanine soln.
Mayer hematoxylin w/ dH2O
borax (blue Mayer)
🗑
|
||||
| show | copper - bright red to red yellow
nuclei - light blue
If copper conc. low, stain may fade after coverslipping
🗑
|
||||
| Microwave Rhodanine method solutions | show 🗑
|
||||
| show | copper - bright red or rust-red intracytoplasmic granules
nuclei - blue
🗑
|
||||
| Working Rhodanine solution | show 🗑
|
||||
| show | A substance that has enough color to be visible w/o any further staining
🗑
|
||||
| show | Melanomas,
Addison's disease
🗑
|
||||
| show | Prussian - ferrocyanide for ferric ions
Turnball - ferricyanide for ferrous ions
🗑
|
||||
| Fontana Masson w/ melanin bleach | show 🗑
|
||||
| show | Gomori methenamine-silver,
Grocott methenamine-silver,
Fontana Masson,
von Kossa
🗑
|
||||
| Argyrophil stains | show 🗑
|
||||
| show | Is a reducing substance,
may give positive rxn to Schmorl & Fontana-Masson
🗑
|
||||
| show | Detection of neutrophils
🗑
|
||||
| show | A characteristic Maltese cross configuration when polarized,
may be introduced from talcum powder of gloves
🗑
|
||||
| Ferrous ions | show 🗑
|
||||
| Ferric ions | show 🗑
|
Review the information in the table. When you are ready to quiz yourself you can hide individual columns or the entire table. Then you can click on the empty cells to reveal the answer. Try to recall what will be displayed before clicking the empty cell.
To hide a column, click on the column name.
To hide the entire table, click on the "Hide All" button.
You may also shuffle the rows of the table by clicking on the "Shuffle" button.
Or sort by any of the columns using the down arrow next to any column heading.
If you know all the data on any row, you can temporarily remove it by tapping the trash can to the right of the row.
To hide a column, click on the column name.
To hide the entire table, click on the "Hide All" button.
You may also shuffle the rows of the table by clicking on the "Shuffle" button.
Or sort by any of the columns using the down arrow next to any column heading.
If you know all the data on any row, you can temporarily remove it by tapping the trash can to the right of the row.
Embed Code - If you would like this activity on your web page, copy the script below and paste it into your web page.
Normal Size Small Size show me how
Normal Size Small Size show me how
Created by:
CCF
Popular Histology sets