Terms from Regulation unit at NW 04-350
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show | some substance in the environment responsible for triggering a change in gene expression. Could be chemical substance, radiation, temperature, pH, or environmental condition
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show | gene that is coding for a metabolic enzyme or protein used to build a structure
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constitutive expression | show 🗑
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show | genes coding for absolutely essential proteins that are constantly being expressed because they are always needed. not regulated
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regulatory product | show 🗑
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show | nucleic acid sequence (DNA or RNA) which the regulatory product attaches, influencing the expression of genes physically connected to the element. cis acting
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show | when a genes expression is stimulated by binding of regulatory product to the regulatory element, but expression is turned off when they do not bind
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negative control | show 🗑
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activator | show 🗑
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repressor | show 🗑
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show | when gene expression is stimulated by the presence of an environmental signal
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repressible | show 🗑
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inducer | show 🗑
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operon | show 🗑
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promoter | show 🗑
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show | common term for a regulatory element adjacent to the promoter. binding site for a regulatory product
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show | cluster of co regulated genes involved in lactose metabolism
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show | gene coding for Beta Galactosidase, the enzyme that breaks lactose into monosaccharides glucose and galactose
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Lac Y | show 🗑
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show | gene coding for trans acetylase
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Lac I | show 🗑
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show | regulatory product involved in positive regulation of lac operon and others as a response to glucose concentration
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cAMP | show 🗑
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show | a small RNA molecule that is the reverse complement of a normal mRNA. the two may hybridize, preventing translation by blocking the ribosomal binding site in prokaryotes
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riboswitch | show 🗑
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show | level of organization of a eukaryotic chromosome, euchromatin vs heterochromatin
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acetylation | show 🗑
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show | removal of an acetyl group
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show | protein produced in female fruit flies that alters the splicing of the tra mRNA, producing a functional protein. males don't express sxl so tra mRNA is spliced in a different way, producing a nonfunctional protein
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gal4 | show 🗑
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show | repressor protein that blocks gal4 from making contact with the mediator, and therefore stops expression of the gal1 gene
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show | repressor of the repressor gal80... therefore an activator. prevents gal80 from blocking contact between gal4 and the mediator. responds to galactose concentration as an environmental signal
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show | name given to the enhancer influencing gal1 expression
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show | technique used to measure the amount of protein present by binding a specific protein with an antibody probe
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show | technique used to measure the amount of RNA present by binding a labelled DNA probe to an RNA
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primary antibody | show 🗑
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show | immunoglobulin that recognizes the shape of a primary antibody. secondary is produced in large volumes to allow conjugation with other substances (enzyme, fluorescent compound, gold particle)
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hybridization | show 🗑
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microarray | show 🗑
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show | enzyme used to convert RNA into its DNA coding sequence, cDNA. done to give a more stable molecule to work with in the lab instead of unstable RNA
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reporter gene | show 🗑
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transgenic organism | show 🗑
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Created by:
jthorns
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