Phlebotomy Order of Draw, Tube colors, & Additives
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| ANTICOAGULANTS (common) | EDTA; Citrate (sodium); Heparin (ammonium, lithium, sodium); Oxalates (potassium)
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| SPECIAL USE ANTICOAGULANTS | Acid Citrate Dextrose (ACD); Citrate-Phosphate-Dextrose (CPD); Sodium Polyanethol Sulfonate (SPS)
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| ANTI-GLYCOLYTIC AGENTS | Sodium Fluoride
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| CLOT ACTIVATORS | Silica (glass); Celite (inert clay); Thrombin; SSTs = Serum separator tubes
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| THIXOTROPIC GEL SEPARATOR | density of gel is between that of cells and serum or plasma
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| TRACE-ELEMENT-FREE TUBES | tubes are made from materials that are as free of trace element contamination as possible; they typically contain EDTA, heparin, or no additive
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| LIGHT BLUE | ADDITIVE=Sodium Citrate//DEPT.=Coagulation
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| RED GLASS | ADDITIVE=none//DEPT.=Chemistry, Blood Bank, Serology, Immunology
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| RED PLASTIC | ADDITIVE=clot activator//DEPT.=Chemistry
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| RED/LIGHT GRAY PLASTIC | ADDITIVE=nonadditive//DEPT.=NA(discard tube only)
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| RED/BLACK(tiger) or GOLD or RED/GOLD | ADDITIVE=clot activator & gel separator//DEPT.=Chemistry
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| GREEN/GRAY or LIGHT GREEN | ADDITIVE=Lithium heparin & gel separator//DEPT.=Chemistry
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| GREEN | ADDITIVE=Lithium Heparin or Sodium Heparin//DEPT.=Chemistry
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| LAVENDER or PINK | ADDITIVE=EDTA//DEPT.=Hematology, Blood Bank
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| GRAY | ADDITIVE=Sodium fluoride or Sodium fluoride & potassium oxalate or Sodium fluoride & EDTA//DEPT.=CHEMISTRY
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| ORANGE or GRAY/YELLOW | ADDITIVE=Thrombin//DEPT.=Chemistry
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| ROYAL BLUE | ADDITIVE=none(red label) or EDTA(lavender label) or sodium heparin(green label)//DEPT.=Chemistry
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| TAN GLASS TUBE | ADDITIVE=Sodium heparin//DEPT.=Chemistry
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| TAN PLASTIC TUBE | ADDITIVE=EDTA//DEPT.=Chemistry(lead levels)
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| YELLOW | ADDITIVE=SPS(sodium polyanethol sulfonate) or ACD(acid citrate dextrose)//DEPT.=Microbiology(SPS) or Blood Bank, Immunohematology(ACD)
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| ORDER OF DRAW (CLSI) | 1.Sterile tube (blood culture) 2.Light-blue top (coagulation tube) 3.Serum tube with or w/out clot activator, with or w/o gel 4.Heparin tube with or w/o gel plasma separator 5.EDTA 6.glycolytic inhibitor
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| ORDER OF DRAW (color tube) | 1.Yellow SPS/Sterile media bottles 2.Lt. blue 3.Red glass 4.Red plastic 5.Gold/red-gray 6.Lt. green/green-gray/tan glass/green 7.Lav./pink/pearl/royal blue/tan plastic 11.Gray
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| PT/PTT | prothrombin time/partial thromboplastin time INVERT 5-8 times-> must draw discard tube if this is first tube drawn!!
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| To get serum, use: | Plain Tubes or Clot Activators (CAT)
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| To get plasma, use: | Anticoagulants
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| BLOOD CULTURES | Yellow SPS, sterile media bottles
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| COAGULATION STUDIES | Light Blue,
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| SERUM STUDIES | Gold, Red Glass, Red Plastic
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| PLASMA STUDIES | HEPARIN -> green,light green,tan glass//EDTA->lavender,pink,royal blue,pearl, tan plastic//O&F->Gray
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| LAVENDER TUBE | Used for CBC
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