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Micro Practical 6/12
| Question | Answer |
|---|---|
| How do you label a Petri dish? | Name, date, time, site, Drs name |
| How do you incubate a Petri dish? | agar side up |
| Culturette- what is the purpose of transport media? | Prevent swab from drying out |
| How do you label a culturette? | Name, date, time, site, Drs name |
| What temp do you incubate at? | 37 degree Celsius |
| What is the isolation streaking technique? | 3 sections. Circle drawings. 8 swipes. 3 stabs in original quadrant |
| What is aseptic technique? | pinky finger on dominant hand is used to take off the cap of tube |
| What stains are used in Gram Stain? | Crystal violet, iodine, gram stain decolorizer, safrinin |
| What microscope objective is used to report smear? | 97x with drop of oil |
| What is SBAP? | Sheep blood agar plate |
| What is alpha? | Green halo |
| What is Beta? | clear around colony |
| What is Gamma? | no change |
| Where is the bacitracin disk placed? | placed in original area |
| What does the bacitracin disk identify? | Group A Strep |
| What equipment is used to Rapid Strep Testing? | 2 swabs, tongue depressor and test kit |
| What does the rapid strep test, test for? | Group A beta hemolytic streptococcus- strep pyogenes |
| What areas do you swab while swabbing the throat? | Tonsillar area, back of throat, include suspicious areas |
| What technique is used to swab the throat? | Figure 8 motion |
| What does Hemoccult test for? | Blood in stool |
| What areas should you get a sample of for a Hemoccult test? | black slimy areas |
| How do you know if a Hemoccult test is positive? | Turns blue |
| What is the patients prep for Hemoccult? | no red or rare meat 2 days prior or during test |
| When do you develop the control on a Hemoccult test? | After results are developed, interrpreted, and recorded |
| What equipment is used to collect a urine culture? | Uricult |
| What media is used to collect a urine culture? | CLEB, EMB |
| How do you interpret the number of organisms on Uricult? | Check against sheet. <10,000, 10,000-100,000 and >100,000 |
| How do you determine the gram stain of an organism on Uricult? | CLED- gram pos/neg EMB- Only gram neg |
| How is a wet mount slide made? | One drop of saline |
| What microscope objectives are used for Wet Mount? | focus on 10X then go to 45X |
| What organisms are seen for a Wet Mount? | Yeast |
| What is the patient prep for O & P? | No laxatives, antacids, or stool softeners for 7 days |
| How is the sample to be collected for 0 & P? | Urinate first, sample must be collected in container. Can NOT be collected from toilet |
| How much do you fill the vial for O & P? | Till red fill line |
Created by:
lecopple1
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